Honeycomb-like architecture produced by living bacteria, Gluconacetobacter xylinus

Uraki, Yasumitsu; Nemoto, Junji; Otsuka, Hiroyuki; Tamai, Yasukatsu; Sugiyama, Junji; Kishimoto, Takao; Ubukata, Makoto; Yabu, Hiroshi; Tanaka, Masaru; Shimomura, Masatsugu

doi:10.1016/j.carbpol.2006.08.021

Cited by 65 publications

(45 citation statements)

References 20 publications

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“…2a and as reported previously by numerous authors. 27,33,34,41,43,46,52 Under high electrical fields, cellulose production ceases. However, there are experimental conditions in which the motion of the bacteria can be controlled while simultaneously producing cellulose.…”

Section: Micro-growth Chamber Cellulose Productionmentioning

confidence: 98%

“…They have also found that bacterial cellulose tubes grown on silicone tubing showed nanofiber orientation along the longitudinal axis. 33 Uraki et al 43 were able to align cellulose nanofibers along honeycomb-patterned microgrooves in an agarose film scaffold. Kondo et al 27 found that bacterial cellulose grown on a molecular substrate of aligned glucan chains resulted in epitaxial-aligned bacterial cellulose nanofibers.…”

Section: Introductionmentioning

confidence: 98%

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Electromagnetically Controlled Biological Assembly of Aligned Bacterial Cellulose Nanofibers

et al. 2010

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We have developed a new biofabrication process in which the precise control of bacterial motion is used to fabricate customizable networks of cellulose nanofibrils. This article describes how the motion of Acetobacter xylinum can be controlled by electric fields while the bacteria simultaneously produce nanocellulose, resulting in networks with aligned fibers. Since the electrolysis of water due to the application of electric fields produces the oxygen in the culture media far from the liquid-air boundary, aerobic cellulose production in 3D structures is readily achievable. Five separate sets of experiments were conducted to demonstrate the assembly of nanocellulose by A. xylinum in the presence of electric fields in micro- and macro-environments. This study demonstrates a new concept of bottom up material synthesis by the control of a biological assembly process.

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Section: Micro-growth Chamber Cellulose Productionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Electromagnetically Controlled Biological Assembly of Aligned Bacterial Cellulose Nanofibers

et al. 2010

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“…Reproduced with permission from ref. [123,127,128,[133][134][135] Sano et al [124] used electric fields to produce oxygen in culture medium. Since G. xylinus is an aerobic bacterium, oxygen induced their ordered motions, which made them biosynthesize ordered cellulose fibers.…”

Section: Microbial Cell Factories: Manufacturing Functional Metabolitesmentioning

confidence: 99%

“…Uniaxially oriented BC fibrils can also be obtained by culturing G. xylinus on poly-di-methyl-siloxane (PDMS) with a ridged topology [126]. When bacteria were cultured on an agarose film scaffold with honeycomb-patterned grooves, a honeycombpatterned BC was obtained [127]. In our research, we utilized ordered agarose or PDMS template as templates to produce micropatterns on BC surface during fermentation.…”

Section: Microbial Cell Factories: Manufacturing Functional Metabolitesmentioning

confidence: 99%

Fabrication of nanocomposites and hybrid materials using microbial biotemplates

Shi

Ullah

et al. 2017

Adv Compos Hybrid Mater

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Microbes are important part of life that vary in sizes and shapes, diverse surface chemistry and biology, and porous nature of their cell walls. Besides their importance in industrial processes such as fermentation, these serve as biotemplates and provide a biomimetic approach for fabrication of multifarious complex constructs with predefined features, ordered composites and hybrid nanomaterials, microdevices, and micro/nanorobots through various strategies. The template or building blocks for such approaches can be bacterial, algal, and fungal cells or virus particles. Here, we have summarized recent advancements in biofabrication based on live microbes. Using engineering approaches and suitable methods, live microbes can be manipulated as functional Bmicro/nanodevices and -robots^to further perform biological functions such as replication, distribution, motility, formation of colonies, and secretion of metabolites at will. Biofabrication based on microbes provides effective methods to control and manipulate microbes as functional live building blocks to create micro/nanodevices and -robots for biomedical and energy applications.

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“…Moreover, the material is characterized by good biocompatibility and biodegradability, so it could be widely applied to the medical, biological, and chemical industries (15).…”

Section: Introductionmentioning

confidence: 99%