Hopanoid lipids: from membranes to plant–bacteria interactions

Belin, Brittany J; Busset, Nicolas; Giraud, Éric; Molinaro, Antonio; Silipo, Alba; Newman, Dianne K.

doi:10.1038/nrmicro.2017.173

Cited by 160 publications

(146 citation statements)

References 141 publications

(116 reference statements)

Supporting

Mentioning

137

Contrasting

Unclassified

Order By: Relevance

“…Biophysical studies, using artificial membranes, have demonstrated that hopanoids decrease the membrane permeability and increase the membrane order without compromising their mechanical properties (Sáenz et al, 2012, 2015; Mangiarotti et al, 2019a). In addition, and in accordance with in vitro studies, hopanoids promote resistance to antibiotics, detergents, extreme pHs, high temperature, oxidation, and high osmolarity in bacteria (Sáenz, 2010; Belin et al, 2018; Welander et al, 2010; Malott et al, 2014; Schmerk et al, 2011; Silipo et al, 2014; Kulkarni et al, 2013).…”

Section: Introductionsupporting

confidence: 70%

Regulation of the activity of an antimicrobial peptide by sterols or hopanoids: possible role in cell recognition

Alvares

Monti

Neto

et al. 2020

Preprint

View full text Add to dashboard Cite

It is now accepted that hopanoids act as sterol-surrogates in membranes of some sterol-lacking bacteria. Here 10 we inquiry whether the hopanoid diplopterol (DP) could attenuate the activity of the antimicrobial peptide Polybia-MP1 11 (MP1) similarly to cholesterol (CHO). Survival of P. aeruginosa exposed to MP1 was lower for cells incubated with DP than 12 those incubated with CHO, and the affinity and subsequent effect of the peptide on lipid bilayers were different in the 13 presence of DP than in the presence of CHO. Membrane properties showed a non-monotonic behavior as the peptide 14 adsorbed, penetrated, and translocated bilayers with DP suggesting a reorganization of MP1 during these processes. We 15 conclude that MP1 selectivity is finely tuned by lipid composition, and propose the differential interaction and consequent 16 effect promoted by the peptide in membranes with diplopterol as a promising starting point for targeting antimicrobial 17 peptides to hopanoid-containing bacterial membranes. 18Introduction 19 Cell membranes are simultaneously compact and fluid. These properties, which are conserved in different species, have 20 been proposed to be those that are compatible with life (Heimburg, 2007), and it is accepted that sterols are inducers 21 of these particular mechanical conditions in different kingdoms. It has been shown in pioneer works and recent publi-22 cations that hopanoids can be considered sterol-surrogates in sterol-lacking bacteria. Biophysical studies, using artificial 23 membranes, have demonstrated that hopanoids decrease the membrane permeability and increase the membrane or-24 der without compromising their mechanical properties (Sáenz et al., 2012(Sáenz et al., , 2015 Mangiarotti et al., 2019a). In addition, 25 and in accordance with in vitro studies, hopanoids promote resistance to antibiotics, detergents, extreme pHs, high tem-26 perature, oxidation, and high osmolarity in bacteria (Sáenz, 2010; Belin et al., 2018; Welander et al., 2010; Malott et al., 27 2014; Schmerk et al., 2011; Silipo et al., 2014; Kulkarni et al., 2013). 28 Given the similarities between membranes with hopanoids and those with sterols, here we compared the activity of a 29 known antimicrobial peptide (AMP), Polybia-MP1 (MP1, IDWKKLLDAAKQIL-NH 2 ), against membranes with diplopterol (DP, 30 a major hopanoid) or cholesterol (CHO). This peptide, extracted from the venom of the Brazilian wasp Polybia paulista, 31 exhibits bactericidal activity against Gram-positive and Gram-negative bacteria being non-hemolytic and non-cytotoxic 32 (Souza et al., 2005). The aim of this study is to get an insight into the reasons for this peptide behavior, deepening the 33 understanding of the dependence of membrane composition on the MP1-membrane interaction. We inquired whether 34 diplopterol protects membranes from peptide action as good as cholesterol, which hinders membrane disruption by 35 MP1 (Dos Santos Cabrera et al., 2008, 2012. We showed using in vivo assays that the presence of DP prevents the a...

show abstract

Section: Introductionsupporting

confidence: 70%

Regulation of the activity of an antimicrobial peptide by sterols or hopanoids: possible role in cell recognition

Alvares

Monti

Neto

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Through the MEP/DOXP pathway, geranyl–geranyl pyrophosphate and farnesyl pyrophosphate are produced and act as respective precursors for squalene and lycopene synthesis. Squalene is a triterpene precursor for the biosynthesis of bacterial hopanoids (Belin et al ., ), which are pentacyclic lipids involved in bacterial membrane fluidity and permeability (Rohmer et al ., ), with growing evidence suggesting roles in plant–bacteria rhizosphere interactions (Belin et al ., ). Lycopene is a precursor for carotenoids, a large family of photoreceptive C 40 polyene pigments produced by photosynthetic bacteria, algae, plants and fungi (Cuttriss et al ., ) which aid in photosynthesis and protection from photooxidation.…”

Section: Resultsmentioning

confidence: 99%

Metabolic relationships of uncultured bacteria associated with the microalgae Gambierdiscus

Rambo

Dombrowski

Constant

et al. 2019

Environmental Microbiology

View full text Add to dashboard Cite

Microbial communities inhabit algae cell surfaces and produce a variety of compounds that can impact the fitness of the host. These interactions have been studied via culturing, single-gene diversity and metagenomic read survey methods that are limited by culturing biases and fragmented genetic characterizations. Higher-resolution frameworks are needed to resolve the physiological interactions within these algalbacterial communities. Here, we infer the encoded metabolic capabilities of four uncultured bacterial genomes (reconstructed using metagenomic assembly and binning) associated with the marine dinoflagellates Gambierdiscus carolinianus and G. caribaeus. Phylogenetic analyses revealed that two of the genomes belong to the commonly algae-associated families Rhodobacteraceae and Flavobacteriaceae. The other two genomes belong to the Phycisphaeraceae and include the first algae-associated representative within the uncultured SM1A02 group. Analyses of all four genomes suggest these bacteria are facultative aerobes, with some capable of metabolizing phytoplanktonic organosulfur compounds including dimethylsulfoniopropionate and sulfated polysaccharides. These communities may biosynthesize compounds beneficial to both the algal host and other bacteria, including iron chelators, B vitamins, methionine, lycopene, squalene and polyketides. These findings have implications for marine carbon and nutrient cycling and provide a greater depth of understanding regarding the genetic potential for complex physiological interactions between microalgae and their associated bacteria.

show abstract

“…As Monk et al remark, the coverage of metabolic reactomes has stagnated, since little effort is spent on comprehensively uncovering the metabolic space of an organism, especially with regards to the secondary metabolism . A thorough analysis of these organisms may lead to interesting discoveries similar to, for instance, that of hopanoid production in M. capsulatus and Alicyclobacillus acidocaldarius …”

Section: Resultsmentioning

confidence: 99%

Microbial Methylotrophic Metabolism: Recent Metabolic Modeling Efforts and Their Applications In Industrial Biotechnology

Lieven

Sonnenschein

2018

Biotechnology Journal

View full text Add to dashboard Cite

Developing methylotrophic bacteria into cell factories that meet the chemical demand of the future could be both economical and environmentally friendly. Methane is not only an abundant, low-cost resource but also a potent greenhouse gas, the capture of which could help to reduce greenhouse gas emissions. Rational strain design workflows rely on the availability of carefully combined knowledge often in the form of genome-scale metabolic models to construct high-producer organisms. In this review, the authors present the most recent genome-scale metabolic models in aerobic methylotrophy and their applications. Further, the authors present models for the study of anaerobic methanotrophy through reverse methanogenesis and suggest organisms that may be of interest for expanding one-carbon industrial biotechnology. Metabolic models of methylotrophs are scarce, yet they are important first steps toward rational strain-design in these organisms.

show abstract

Hopanoid lipids: from membranes to plant–bacteria interactions

Cited by 160 publications

References 141 publications

Regulation of the activity of an antimicrobial peptide by sterols or hopanoids: possible role in cell recognition

Regulation of the activity of an antimicrobial peptide by sterols or hopanoids: possible role in cell recognition

Metabolic relationships of uncultured bacteria associated with the microalgae Gambierdiscus

Microbial Methylotrophic Metabolism: Recent Metabolic Modeling Efforts and Their Applications In Industrial Biotechnology

Contact Info

Product

Resources

About