Hormonal regulation of apoptosis in the endometrium of common marmosets (Callithrix jacchus)

Narkar, Madhura; Kholkute, S. D.; Nandedkar, Tarala D.

doi:10.1016/j.theriogenology.2006.03.032

Cited by 10 publications

(6 citation statements)

References 56 publications

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“…Cells undergoing apoptosis were more dominant in mid‐luteal phase when compared with early and late follicular phase. Assessment of apoptosis in ovariectomized marmoset monkeys treated with either estradiol alone or progesterone alone or estradiol followed by progesterone revealed induction of apoptosis in endometrial cells only in the latter group (Narkar et al,2006), indicating a causal relation of this event and cyclic ovarian changes, such as follicular development followed by ovulation and luteal development, as found in the canine oviduct in our study.…”

Section: Discussionsupporting

confidence: 70%

Apoptosis in the Uterotubal Junction and Oviductal Isthmus during the Estrous Cycle of the Bitch

Urhausen

Beineke

Marion

et al. 2010

The Anatomical Record

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Apoptotic cells were evaluated in the lamina epithelialis (LE) and lamina propria (LP) of the canine uterotubal junction and oviductal isthmus by means of the TUNEL assay and Caspase-3 detection regarding cyclic changes. Oviducts of bitches in anestrus, early and late follicular phase, 2 and 4 days after ovulation, mid-luteal phase, and endometrial reparation were available. The rate of apoptosis was generally low (min ¼ 0; max ¼ 33 apoptotic cells in 500 cells). Regardless of the stage of the estrous cycle, the number of apoptotic cells was significantly higher in the LE compared to the LP (P < 0.05). In anoestrus, early and late follicular phase as well as during endometrial reparation, the number of apoptotic cells was on a constantly low level. A significant increase was found 4 days after ovulation by caspase-3 detection coinciding with early luteal development and in mid-luteal phase. The results demonstrate that the number of apoptotic cells changes depending on the stage of the ovarian cycle. The coincidence of the increase in the number of apoptotic cells and the time of achievement of oocyte competence, which is known to occur in the bitch 3-4 days after ovulation, suggests a causal relationship between the postovulatory maturation stage of oocytes and the rate of apoptosis in the caudal oviduct. Furthermore, a specific relevance of apoptosis may exist in the process of fertilization regarding sperm storage and detachment of spermatozoa bound to the epithelium of the caudal oviduct, which has been identified as a functional sperm reservoir in other species. Anat Rec, 294:342-348, 2011. V V C 2010 Wiley-Liss, Inc.Key words: apoptosis; oviduct; uterotubal junction; isthmus; sperm reservoirThe oviduct plays an important role in reproduction by providing a suitable environment for oocyte pick up and maturation, sperm transport and storage, capacitation of spermatozoa, fertilization, and finally early embryonic development (Verhage et al., 1973a;Steinhauer et al., 2004;Rodriguez-Martinez, 2007). The canine estrous cycle differs considerably from that of other species, in that the follicular phase is prolonged and ovulation is followed by a prolonged luteal phase with an average duration of about 54-70 days in the nonpreg-*Correspondence to:

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Section: Discussionsupporting

confidence: 70%

Apoptosis in the Uterotubal Junction and Oviductal Isthmus during the Estrous Cycle of the Bitch

Urhausen

Beineke

Marion

et al. 2010

The Anatomical Record

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“…One of the basic events of uterine cellular change is physiological cell turnover, which involves cell death by apoptosis and cell renewal by proliferation (Spencer et al 1996;Toki et al 1998). Previous studies in several mammalian species have demonstrated an inverse correlation between cell apoptosis and cell proliferation in the uterus (Kokawa et al 1996;Sato et al 1997;Tao et al 1998;Pampfer and Donnay 1999;Dharma et al 2000;Vaskivuo et al 2000;Chu et al 2002Chu et al , 2006Joswig et al 2003;Van Cruchten et al 2003;Harada et al 2004;Wei et al 2005;Narkar et al 2006;Zhang and Paria 2006;Okano et al 2007; Roberto da Costa et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Expression of Survivin, Bcl‐2 and Bax Proteins in the Domestic Cat (Felis catus) Endometrium During the Oestrus Cycle

Lïman¹,

Alan²,

Bayram³

et al. 2012

Reprod Domestic Animals

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Contents Apoptosis has been shown to be an important regulator of endometrium function. To clarify the regulation of apoptosis in the cat endometrium during the normal oestrus cycle, the expressions of the apoptosis‐related proteins (Bcl‐2 and Bax) and their correlation to the inhibitor of apoptosis protein Survivin were analysed using immunohistochemistry. The TUNEL technique (TdT‐mediated dUTP nick end labelling) was also used to detect DNA fragmentation characteristic of apoptotic cells. The results demonstrated that TUNEL labelling is not effective for the detection of apoptosis in cat endometrium. Survivin was expressed in the luminal and glandular epithelial cells of cat endometrium during all phases of the oestrus cycle. Survivin was localized in both the cytoplasm and nuclei of superficial and deep uterine gland cells during the luteal phase, while only cytoplasmic staining was observed during the follicular and anoestrus phases. Bax immunoreactivity in the cytoplasm of luminal and glandular epithelial cells as well as the smooth muscle cells of blood vessels was weak in the anoestrus phase. Compared with anoestrus, the intensity of Bax immunostaining was moderate in the follicular phase and increased dramatically in the luteal phase. Bcl‐2 immunostaining in the cytoplasm of luminal and glandular epithelial cells was moderate in the anoestrus phase. During the early follicular phase, cytoplasmic Bcl‐2 immunostaining was detected mostly in glandular epithelial cells. In the mid‐follicular phase, in glands, the amount of Bcl‐2 protein increased progressively from the superficial to the deep layer. In contrast, the expression of Bcl‐2 decreased in the secretory phase, being very low or absent in the mid‐ and late luteal phases. The overall results suggest that Survivin, Bax and Bcl‐2 proteins may cooperatively contribute to cell apoptosis and cell proliferation in the cat uterus during the oestrus cycle.

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“…The activation of endonucleases, and subsequent cleavage of DNA, is a definitive step toward apoptosis, but there are many factors that can play a role in earlier stages to inhibit the entire apoptotic process, such as variation in hormones levels, modifications of the cytoskeleton or loss of cell anchorage (Frisch and Francis 1994;Janz et al 1997;Kulms et al 2002). In mammalian ovaries, many studies indicate that 17b estradiol can act as an anti-apoptotic factor for granulosa cells (Guthrie et al 1995;Chun et al 1996;Narkar et al 2006). The relationship between follicular apoptosis and plasma 17b estradiol levels in the reproductive dynamics of teleosts has been investigated in a few species, for example Oncorhyncus mykiss (Janz et al 1997) and Chalcalburnus tarichi (Kaptaner and Ural 2006).…”

Section: Introductionmentioning

confidence: 99%

Distribution of laminin β2, collagen type IV, fibronectin and MMP-9 in ovaries of the teleost fish

Thomé

Santos

Sato³

et al. 2010

J Mol Hist

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Extracellular matrix in the ovarian follicle has been characterised for several mammalian species but there are no reports that describe the immunolocalisation of the extracellular matrix elements, matrix metalloproteinases, and its relation to plasma 17β estradiol levels and follicular apoptosis during the teleost's reproductive cycle. The present study used immunohistochemistry to characterise the distribution of laminin β2, collagen type IV, fibronectin and matrix metalloproteinases-9 (MMP-9). The TUNEL in situ technique was used to quantify apoptosis and indirect immunofluorimetric to determine plasma 17β estradiol levels. The TUNEL-positive reaction associated with morphological features exhibited follicular apoptosis. During postovulatory follicle involution, the drop in plasma 17β estradiol levels after spawning contributed to the intense apoptosis observed. By immunohistochemical analysis, laminin β2 and collagen type IV were identified as the major constituents of the basement membrane. The loss of integrity of the basement membrane occurred due to lyses of the major constituents, and coincides with increased follicular apoptosis. The integrity of the basement membrane is important for the survival of follicular cells. Furthermore, the MMP-9 results suggest that this enzyme is involved in final oocyte maturation and regression of postovulatory follicles. Fibronectin was observed on the surface of follicular cells of the postovulatory follicle in P. argenteus, this being important for maintaining normal cell adhesion to extracellular matrix. In conclusion, our results suggest that the structure and composition of the extracellular matrix, and plasma 17β estradiol levels related to apoptosis, play an important role during the follicular development and post-spawning involution in teleost fishes.

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Hormonal regulation of apoptosis in the endometrium of common marmosets (Callithrix jacchus)

Cited by 10 publications

References 56 publications

Apoptosis in the Uterotubal Junction and Oviductal Isthmus during the Estrous Cycle of the Bitch

Apoptosis in the Uterotubal Junction and Oviductal Isthmus during the Estrous Cycle of the Bitch

Expression of Survivin, Bcl‐2 and Bax Proteins in the Domestic Cat (Felis catus) Endometrium During the Oestrus Cycle

Distribution of laminin β2, collagen type IV, fibronectin and MMP-9 in ovaries of the teleost fish

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