Hormonal stimulation of starfish oocytes induces partial degradation of the 3′ termini of <i>cyclin B</i> mRNAs with oligo(U) tails, followed by poly(A) elongation

Ochi, Hiroe; Chiba, Kazuyoshi

doi:10.1261/rna.054882.115

Cited by 13 publications

(19 citation statements)

References 52 publications

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“…After fertilization, the rate of de novo protein synthesis is accelerated with a considerable time lag for most animal species [ 24 , 100 – 105 ], as mRNAs get unmasked, polyadenylated, or engaged with active polysomes [ 106 – 108 ]. Other post-transcriptional controls such as polyadenylation or partial degradation of the transcripts during meiotic maturation and fertilization may also contribute to translatability of the mRNAs [ 109 , 110 ]. Hence, quantitative characterization of the transcriptome alone does not provide sufficient information on biochemical activities of the eggs and early embryos.…”

Section: Discussionmentioning

confidence: 99%

De novo assembly of a transcriptome from the eggs and early embryos of Astropecten aranciacus

Musacchia¹,

Vasilev²,

Borra³

et al. 2017

PLoS ONE

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Starfish have been instrumental in many fields of biological and ecological research. Oocytes of Astropecten aranciacus, a common species native to the Mediterranean Sea and the East Atlantic, have long been used as an experimental model to study meiotic maturation, fertilization, intracellular Ca2+ signaling, and cell cycle controls. However, investigation of the underlying molecular mechanisms has often been hampered by the overall lack of DNA or protein sequences for the species. In this study, we have assembled a transcriptome for this species from the oocytes, eggs, zygotes, and early embryos, which are known to have the highest RNA sequence complexity. Annotation of the transcriptome identified over 32,000 transcripts including the ones that encode 13 distinct cyclins and as many cyclin-dependent kinases (CDK), as well as the expected components of intracellular Ca2+ signaling toolkit. Although the mRNAs of cyclin and CDK families did not undergo significant abundance changes through the stages from oocyte to early embryo, as judged by real-time PCR, the transcript encoding Mos, a negative regulator of mitotic cell cycle, was drastically reduced during the period of rapid cleavages. Molecular phylogenetic analysis using the homologous amino acid sequences of cytochrome oxidase subunit I from A. aranciacus and 30 other starfish species indicated that Paxillosida, to which A. aranciacus belongs, is not likely to be the most basal order in Asteroidea. Taken together, the first transcriptome we assembled in this species is expected to enable us to perform comparative studies and to design gene-specific molecular tools with which to tackle long-standing biological questions.

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Section: Discussionmentioning

confidence: 99%

De novo assembly of a transcriptome from the eggs and early embryos of Astropecten aranciacus

Musacchia¹,

Vasilev²,

Borra³

et al. 2017

PLoS ONE

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“… 172 ) Very recently, Kazuyoshi Chiba and colleagues found an interesting regulatory mechanism of poly(A)-tail elongation for protein synthesis during starfish oocyte maturation. 173 ) My own group is now focusing our research on two areas: the identification of the 1-MeAde receptor and characterization of the starfish Asterina pectinifera genome (in collaboration with Takehiko Itoh). Based on the experiences recounted here, we anticipate that the completion of these studies will open new frontiers that will extend well beyond the starfish.…”

Section: Concluding Remarks: Lessons From Starfish Oocytesmentioning

confidence: 99%

MPF-based meiotic cell cycle control: Half a century of lessons from starfish oocytes

Kishimoto

2018

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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In metazoans that undergo sexual reproduction, genomic inheritance is ensured by two distinct types of cell cycle, mitosis and meiosis. Mitosis maintains the genomic ploidy in somatic cells reproducing within a generation, whereas meiosis reduces by half the ploidy in germ cells to prepare for successive generations. The meiotic cell cycle is believed to be a derived form of the mitotic cell cycle; however, the molecular mechanisms underlying both of these processes remain elusive. My laboratory has long studied the meiotic cell cycle in starfish oocytes, particularly the control of meiotic M-phase by maturation- or M phase-promoting factor (MPF) and the kinase cyclin B-associated Cdk1 (cyclin B-Cdk1). Using this system, we have unraveled the molecular principles conserved in metazoans that modify M-phase progression from the mitotic type to the meiotic type needed to produce a haploid genome. Furthermore, we have solved a long-standing enigma concerning the molecular identity of MPF, a universal inducer of M-phase both in mitosis and meiosis of eukaryotic cells.

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“…This is ensured by the RNA exosome assisted by the SKI complex (figure 8). [3,13,31,[76][77][78][79][80][81][82][83]. Tailing oligo(A) tails with a few uridines likely facilitates the binding of LSm1-7 complex, which binds preferentially to short oligo(A) tails and oligo(U) tails [72,[84][85][86].…”

Section: Uridylation Of 5 0 Fragments Of Mrnas Cleaved By Riscmentioning

confidence: 99%

RNA uridylation and decay in plants

Almeida

Scheer

Gobert

et al. 2018

Phil. Trans. R. Soc. B

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RNA uridylation consists of the untemplated addition of uridines at the 3′ extremity of an RNA molecule. RNA uridylation is catalysed by terminal uridylyltransferases (TUTases), which form a subgroup of the terminal nucleotidyltransferase family, to which poly(A) polymerases also belong. The key role of RNA uridylation is to regulate RNA degradation in a variety of eukaryotes, including fission yeast, plants and animals. In plants, RNA uridylation has been mostly studied in two model species, the green algae Chlamydomonas reinhardtii and the flowering plant Arabidopsis thaliana . Plant TUTases target a variety of RNA substrates, differing in size and function. These RNA substrates include microRNAs (miRNAs), small interfering silencing RNAs (siRNAs), ribosomal RNAs (rRNAs), messenger RNAs (mRNAs) and mRNA fragments generated during post-transcriptional gene silencing. Viral RNAs can also get uridylated during plant infection. We describe here the evolutionary history of plant TUTases and we summarize the diverse molecular functions of uridylation during RNA degradation processes in plants. We also outline key points of future research. This article is part of the theme issue ‘5′ and 3′ modifications controlling RNA degradation’.

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Hormonal stimulation of starfish oocytes induces partial degradation of the 3′ termini of cyclin B mRNAs with oligo(U) tails, followed by poly(A) elongation

Cited by 13 publications

References 52 publications

De novo assembly of a transcriptome from the eggs and early embryos of Astropecten aranciacus

De novo assembly of a transcriptome from the eggs and early embryos of Astropecten aranciacus

MPF-based meiotic cell cycle control: Half a century of lessons from starfish oocytes

RNA uridylation and decay in plants

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