Host microRNA analysis in cyprinid Herpesvirus-3 (CyHV-3) infected common carp

Reichert, M.; Łukasik, Anna; Zielenkiewicz, Piotr; Matras, Marek; Maj‐Paluch, Joanna; Stachnik, Magdalena; Borzym, Ewa

doi:10.1186/s12864-018-5266-9

Cited by 9 publications

(9 citation statements)

References 56 publications

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“…Forty-five healthy juvenile carp was infected by CyHV-3, and the carps showed three different phases after infection: control, clinical and latent. 27 and fivee miRNAs were differentially expressed in the clinical or latent phase compared to the control phase (Reichert et al 2019). The miRNAs regarding on another Alloherpesviridae family member Cyprinid herpesvirus 2 (CyHV-2) have also been studied.…”

Section: Herpesviridaementioning

confidence: 99%

MicroRNA regulation of viral replication in teleost fish: a review

Zhang

2021

Reviews in Aquaculture

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MicroRNAs (miRNAs) are single-stranded non-coding RNA molecules. In addition to participating in various biological processes of eukaryotes, such as cell proliferation, development, differentiation, metabolism, tumorigenesis, and apoptosis, miRNAs also play an important role in the regulation of virus replication. Here, we review the current knowledge on high-throughput miRNA sequencing of teleost fish or fish cells in response to virus infection, the role of teleost fish miRNAs in regulating virus replication via targeting either fish or virus genes and the function of teleost fish virus-encoded miRNAs during virus infection. Understanding the interaction between the miRNAs and the host or virus genes will lead to the identification of novel strategy to prevent virus infection in teleost fish.

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Section: Herpesviridaementioning

confidence: 99%

MicroRNA regulation of viral replication in teleost fish: a review

Zhang

2021

Reviews in Aquaculture

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“…The expression level of ORF121 protein was measured by Western blot. GiCF cells were transfected with CyHV-2- KT-635 mimics, mimics-NC, inhibitor, and inhibitor NC at 24 hr and 48 hr et al, 2011;Michal et al, 2019;Wai-Ming et al, 2009;Yates et al, 2013). The 8 known miRNAs abundance were much lower, comparing to the 2 novel viral miRNAs (CyHV-2-KT-635 and CyHV-2-KT-698) in CyHV-2-infected GiCF cells.…”

Section: F I G U R Ementioning

confidence: 99%

“…Reichert (Michal et al, 2019) analysed common carp microRNAs engaged during CyHV-3 infection. Owen H. Donohoe predicted and identified CyHV-3 miRNA MR5057-miR-3p, which targets the 3' UTR of CyHV-3 orf123 gene encoding the viral dUTPase (Donohoe et al, 2015).…”

mentioning

confidence: 99%

Integrated analysis of viral miRNAs, mRNA and protein in the caudal fin cells of C. auratus gibelio with cyprinid herpesvirus 2 infection

Luo

Feng

et al. 2021

Journal of Fish Diseases

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Cyprinid herpesvirus 2(CyHV-2), also called goldfish haematopoietic necrosis virus (G FHNV), was first identified from goldfish in Japan (Jung & Miyazaki, 1995). The virus propagated with extreme rapidity and caused the outbreak of severe disease breaks out in the east of China, resulting in serious economic losses (Wang et al., 2012). CyHV-2 is the double-stranded DNA virus (175-220 nm) consisting of a hexagonal nuclear crust, cystic membrane, and a cyst (Groff et al., 1998). CyHV-2 is the smallest fish herpesvirus with a genome

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“…Carp that survive a primary infection with CyHV-3 can be resistant to future infection with this virus. Since latency and persistent carrying of CyHV-3 exist in carp (2–4), genetic backgrounds are crucial in developing an understanding resistance against the virus. Experimental infections of carp from pure lines or crosses have indicated the existence of genetic background of resistance by divergent survival rates (2).…”

Section: Introductionmentioning

confidence: 99%

Integrative Transcriptomic Analysis Reveals the Immune Mechanism for A CyHV-3-Resistant Common Carp Strain

Jia

Jiang

et al. 2020

Preprint

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Anti-disease breeding is becoming the most promising solution to cyprinid herpesvirus-3 (CyHV-3) infection, the major threat to common carp aquaculture. Mortality studies suggested that a breeding strain of common carp is resistant to this disease. This study illustrates the immune mechanisms involved in anti-CyHV-3 ability. An integrative analysis of the protein-coding genes and long non-coding RNAs (lncRNAs) using transcriptomic data was also performed. Tissues from the head kidney of common carp were extracted at day 0 (the healthy control) and day 7 after CyHV-3 infection (the survivors), and used to analyze the transcriptome through both Illumina and Pac-bio sequencing. Following analysis of the Kyoto Encyclopedia of Genes and Genomes pathways and Gene Ontology terms involved, the immune-related genes were merged. These genes were filtered using the current common carp immune gene library, and information on the immune process was detailed. Immune gene categories and their corresponding genes in different comparison groups were revealed. Also, the immunological Gene Ontology terms for lncRNA modulation were retained. The weighted gene co-expression network analysis was used for the regulation of immune genes lncRNA. The results demonstrated that the breeding carp strain develops marked resistance to CyHV-3 through a specific innate immune mechanism. The featured biological processes were autophagy, phagocytosis, cytotoxicity, and virus blockage by lectins and mucin 3 (MUC3). Moreover, the immune suppressive signals, such as suppression of interleukin 21 receptor (IL21R) on STAT3, PI3K mediated the inhibition of inflammation by dopamine upon infection, as well as the inhibition of NLR family CARD domain containing 3 (NLRC3) on STING during a steady state. Possible susceptible factors for CyHV-3, such as integrin beta 1 (ITGB1), toll-like receptor 18 (TLR18), and C-C motif chemokine ligand 4 (CCL4), were also revealed from the common strain. The results of this study suggested that the regulation of galectin 3 (LGALS3) and T cell leukemia homeobox 3 (TLX3) by lncRNA may play a role in the resistance mechanism. Therefore, immune factors that are immunogenetically insensitive or susceptible to CyHV-3 infection have been revealed.

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Host microRNA analysis in cyprinid Herpesvirus-3 (CyHV-3) infected common carp

Cited by 9 publications

References 56 publications

MicroRNA regulation of viral replication in teleost fish: a review

MicroRNA regulation of viral replication in teleost fish: a review

Integrated analysis of viral miRNAs, mRNA and protein in the caudal fin cells of C. auratus gibelio with cyprinid herpesvirus 2 infection

Integrative Transcriptomic Analysis Reveals the Immune Mechanism for A CyHV-3-Resistant Common Carp Strain

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