Host Range Studies of Soybean Mosaic Virus

Nandakishor, H. V.; Kumaraswamy, B.; Mane, S.S.; Veena, G Amrutha

doi:10.20546/ijcmas.2017.607.035

Cited by 3 publications

(1 citation statement)

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“…SMV can be seed transmitted or by aphid vectors (Li et al, 2018). Besides soybean, it can infect other legumes including pea and was found in German faba beans before (Nandakishor et al, 2017). WCCV2, a symptomless cryptic virus, has been previously detected in German in white clover (Trifolium repens) (Lesker et al, 2013) and in many other countries like Japan, Korea, New Zealand and USA (Zhao et al, 2016;Ido et al, 2012;Park et al, 2017;Nakabayashi et al, 2002;Bos et al, 1959).…”

Section: Chapter 3: Pea Viromementioning

confidence: 99%

Plant virus identification and virus-vector-host interactions

Gaafar¹

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BackgroundNucleorhabdoviruses possess bacilliform particles which contain a single-stranded negative-sense RNA genome. They replicate and mature in the nucleus of infected cells. Together with viruses of three other genera of the family Rhabdoviridae, they are known to infect plants and can be transmitted by arthropod vectors, during vegetative propagation, or by mechanical means. In 2010, an alfalfa (Medicago sativa) plant showing virus-like symptoms was collected from Stadl-Paura, Austria and sent to Julius Kühn Institute for analysis. MethodsElectron microscopy (EM) of leaf extracts from infected plants revealed the presence of rhabdovirus-like particles and was further used for ultrastructural analyses of infected plant tissue. Partially-purified preparations of rhabdovirus nucleocapsids were used for raising an antiserum. To determine the virus genome sequence, high throughput sequencing (HTS) was performed. RT-PCR primers were designed to confirm virus infection and to be used as a diagnostic tool. ResultsEM revealed bacilliform virions resembling those of plant-infecting rhabdoviruses. HTS of ribosomal RNA-depleted total RNA extracts revealed a consensus sequence consisting of 13,875 nucleotides (nt) and containing seven open reading frames (ORFs). Homology and phylogenetic analyses suggest that this virus isolate represents a new species of the genus Nucleorhabdovirus (family Rhabdoviridae). Since the virus originated from an alfalfa plant in Austria, the name alfalfa-associated nucleorhabdovirus (AaNV) is proposed. Viroplasms (Vp) and budding virions were observed in the nuclei of infected cells by EM, thus confirming its taxonomic assignment based on sequence data. ConclusionsIn this study, we identified and characterised a new nucleorhabdovirus from alfalfa. It shared only 39.8% nucleotide sequence identity with its closest known relative, black currant-associated rhabdovirus 1. The virus contains an additional open reading frame (accessory gene) with unknown function, located between the matrix protein and the glycoprotein genes. Serological and molecular diagnostic assays were designed for future screening of field samples. Further studies are needed to identify other natural hosts and potential vectors.

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Section: Chapter 3: Pea Viromementioning

confidence: 99%

Plant virus identification and virus-vector-host interactions

Gaafar¹

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An Overview on Soybean Mosaic Virus and Its Management

Srivastava

Bhardwaj

Choudhary

et al. 2022

Plant Stress Mitigators

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Incidence of viral disease mosaic symptom and vector insects’ presence in several soybean varieties in pine agroforestry system

Supyani,

Septariani,

Cahya

2024

IOP Conf. Ser.: Earth Environ. Sci.

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Pine dan soybean agroforestry represents an initiative to enhance land productivity and yield. Nevertheless, various viruses could infect soybeans in agroforestry. Mosaic disease is a predominant viral disease affecting soybeans. This research was conducted at KHDTK (Kawasan Hutan dengan Tujuan Khusus) Forestry Alas Bromo in Karanganyar Regency, Central Java, Indonesia. The study aimed to observe the disease incidence and vector presence in some soybean varieties in the pine agroforestry system. A Randomized Block Design was used for the experiment, encompassing seven treatments of varieties. The varieties were Anjasmoro, Argomulyo, Dena-1, Dena-2, Denasa-1, Denasa-2, and Devon-1. Planted at 2.4 x 3.6 m and replicated three times. The incidence of mosaic disease is observed based on symptoms, especially on the leaves’ surface in the form of chlorosis with mosaic pattern. Results indicated that mosaic symptoms were found in the field and the disease incidence was varied for each variety. Viral vectors visited the soybean varieties: Aphis glycine, Phenacoccus sp., thrips, and Bemisia tabaci.

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Host Range Studies of Soybean Mosaic Virus

Cited by 3 publications

References 0 publications

Plant virus identification and virus-vector-host interactions

Plant virus identification and virus-vector-host interactions

An Overview on Soybean Mosaic Virus and Its Management

Incidence of viral disease mosaic symptom and vector insects’ presence in several soybean varieties in pine agroforestry system

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