Hot-Water Treatment before Tissue Culture Reduces Initial Contamination in Lilium and Acer

Langens-Gerrits, Merel; Albers, M.R.J.; Klerk, G.J.M. de

doi:10.1007/978-94-015-8951-2_27

Cited by 16 publications

(8 citation statements)

References 9 publications

(10 reference statements)

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“…Our study showed that the use of immature embryos in in vitro multiplication studies of M. azureum resulted in a high frequency of bulblet production. Furthermore, the use of underground storage organs as a source causes fungal contamination of in vitro culture (Langens-Gerrits et al, 1998;Ziv and Lilien-Kipnis 2000;Mirici et al, 2005). No contamination was observed in the immature embryo explants of M. azureum in our study.…”

Section: Discussionmentioning

confidence: 50%

In vitro bulblet regeneration from immature embryos of endangered and endemic Muscari azureum

Uranbey

2011

ARCH BIOL SCI BELGRA

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A high frequency of bulblet regeneration was achieved for the endemic and endangered ornamental plant Muscari azureum using immature embryos. Immature embryos of M. azureum were cultured on a callus induction medium consisting of N6 mineral salts and vitamins, 400 gL-1 casein + 40 gL-1 sucrose + 2 mgL-1 L-proline, 2 mgL-1 2,4-D and 2 gL-1 Gelrite. Then the embryogenic callus clusters were transferred to a bulblet induction medium consisting of MS mineral salts and vitamins containing different concentrations and combinations of BAP, KIN, TDZ, Zeatin, IAA, NAA, 30 gL-1 sucrose and 7 gL-1 agar. Prolific bulblet multiplication (over 13 bulblets/embryo) was achieved from immature embryos after 5-6 months of culture initiation. Well-developed bulblets were excised and individually rooted on Ѕ strength MS medium supplemented with 1 mgL-1 IBA, 0.5 gL-1activated charcoal, 20 gL-1sucrose and 6 gL-1agar and acclimatized

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Section: Discussionmentioning

confidence: 50%

In vitro bulblet regeneration from immature embryos of endangered and endemic Muscari azureum

Uranbey

2011

ARCH BIOL SCI BELGRA

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“…The use of bulbs as a source of explant is often associated with heavy bacterial and fungal contamination (Langens-Gerrits et al, 1998;Ziv and Lillien-Kipnis, 2000). However, contamination was not observed on cultured immature embryos, which can be excellent sources for contaminationfree explants.…”

Section: Discussionmentioning

confidence: 99%

Efficient in vitro bulblet regeneration from immature embryos of endangered Sternbergia fischeriana

Mirici

Parmaksız

Özcan

et al. 2005

Plant Cell Tiss Organ Cult

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Sternbergia fischeriana is an endangered geophyte and therefore in vitro micropropagation of this plant will have great importance for germplasm conservation and commercial production. Bulb scale and immature embryo explants of S. fischeriana were cultured on different nutrient media supplemented with various concentrations of plant growth regulators. Immature embryos produced higher number of bulblets than bulb scales. Large numbers of bulblets were regenerated (over 80 bulblets/explants) from immature embryos on Murashige and Skoog (MS) medium supplemented with 4 mg l )1 6-benzylaminopurine (BA) and 0.25 mg l )1 a-naphthaleneacetic (NAA) or 2 mg l )1 2,4-dichlorophenoxyacetic acid (2,4-D D ) after 14 months of culture initiation. Regenerated bulblets were kept at 5°C for 5 weeks and then transplanted to a potting mixture.

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“…However, the use of bulbs as starting material is often associated with heavy bacterial or fungal contamination (Langens-Gerrits et al, 1998;Ziv and Liliens-Kipnis, 2000;Karagüzel et al, 2012;Liu et al, 2012). Moreover, collection of bulbs from wild flora and their use for propagation purposes could have negative impacts on endangered plant species.…”

Section: Discussionmentioning

confidence: 99%

In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

Uzun¹,

Parmaksız²,

Uranbey³

et al. 2014

Turk J Biol

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An efficient in vitro bulblet production procedure from immature zygotic embryos of endemic and endangered Muscari muscarimi Medik. was described in the current study. Zygotic embryos were first isolated from immature seeds and cultured on different nutrient media compositions supplemented with various combinations of α-naphthalene acetic acid (NAA), picloram, dicamba, 6-benzylaminopurine (BAP), and thidiazuron (TDZ). The best bulblet regeneration (59 bulblets per explant) was achieved in Murashige and Skoog (MS) medium containing 4 mg/L BAP and 0.5 mg/L NAA after 1 year of culture initiation. Regenerated bulblets were then transferred into MS medium without plant growth regulators for rooting. Bulblets produced well-developed root systems and increased their size on this medium after 2 months. All rooted bulblets were successfully transplanted into a potting mixture and acclimatized to ambient conditions.

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Hot-Water Treatment before Tissue Culture Reduces Initial Contamination in Lilium and Acer

Cited by 16 publications

References 9 publications

In vitro bulblet regeneration from immature embryos of endangered and endemic Muscari azureum

In vitro bulblet regeneration from immature embryos of endangered and endemic Muscari azureum

Efficient in vitro bulblet regeneration from immature embryos of endangered Sternbergia fischeriana

In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

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