Urinary tract infections (UTIs) are a common health problem. They are caused by bacteria from the environment getting into the urinary tract. A total of 118 urine samples were collected from 102 patients (Men and Women) with UTIs admitted to a general hospital in AL-Najaf City, Iraq, between October to 2022 and February 2023. Standard laboratory methods were used to culture urine and to diagnose all bacterial isolates that were grown on laboratory media. The Kirby-Bauer disc diffusion method was used to test antibiotic susceptibility. Out of a total of 118 urine samples there were 106 urine samples with positive bacterial growth and 12 urine samples without bacterial growth. E. coli was the most predominant bacteria with 50 isolates, K. pneumoniae 18, P. mirabilis 13, S. saprophyticus 10, S. aureus 9, E. faecalis 6. Gram-negative bacterial isolates were good resistance to amoxicillin 25 μg and Amoxicillin+clavulanic acid 25/10μg. Gram-positive bacterial isolates were good resistance to penicillin 6 μg and ampicillin 10 μg. The most effective antibiotic was imipenem 10μg, which inhibited all bacterial growth.