How to screen non-viral gene delivery systems in vitro?

Polycationic star polymers with hyperbranched cores for gene delivery

“…DMEM contains inorganic salts, amino acids, vitamins, glucose and, in a 5% CO 2 environment, possesses neutral pH. The viscosity (0.08228 cP) and refractive index (1.4) of DMEM at 25 °C was taken into account for the calculations in the DLS measurements [43].…”

“…A 4784 base pair plasmid DNA coding luciferase (pMetLuc2-Control) was used. An appropriate amount of star solution in DMEM was added to a fixed amount of DNA, which is more efficient than adding in the reverse order [43]. The solutions were incubated for 30 minutes at room temperature.…”

Dual-degradable disulfide-containing PEI–Pluronic/DNA polyplexes: transfection efficiency and balancing protection and DNA release

“…[12][13][14] Unfortunately, transfection efficiency and cytotoxicity appear to be related; PEI with low molecular weight (LMW) (ie, molecular weight = 800 Da, 2,000 Da, or less) exhibits lower cytotoxicity and lower transfection efficiency, whereas PEI with high molecular weight (ie, 25 kDa) exhibits higher transfection efficiency but also higher cytotoxicity. 15,16 Currently, efforts are being undertaken to decrease cytotoxicity and improve the transfection efficiency of gene vectors by cross-linking LMW units via linkages that are potentially degradable.…”

A peptide-mediated targeting gene delivery system for malignant glioma cells

“…[11][12][13] PEI is considered one of the good nonviral vectors, and it shows higher transfection efficiency than PLL and chitosan. [14][15][16] However, the PEI/DNA nanoparticles are still less effective than viral vectors and have no targeting effect. 14,16,17 Certain molecules overexpressed on the surface of some tumor cells, including fibroblast growth factor receptors and epidermal growth factor receptors, have been used to improve the penetration of drugs or genetic products into tumor cells.…”

“…[14][15][16] However, the PEI/DNA nanoparticles are still less effective than viral vectors and have no targeting effect. 14,16,17 Certain molecules overexpressed on the surface of some tumor cells, including fibroblast growth factor receptors and epidermal growth factor receptors, have been used to improve the penetration of drugs or genetic products into tumor cells. [18][19][20] However, these molecules are not specific enough because they are also expressed on some normal cells, although not to the same extent as on tumor cells.…”