HPLC-DAD-ESI-QTOF-MS and HPLC-FLD-MS as valuable tools for the determination of phenolic and other polar compounds in the edible part and by-products of avocado

López-Cobo, Ana; Gómez-Caravaca, Ana María; Pasini, Federica; Caboni, Maria; Segura‐Carretero, Antonio; Fernández‐Gutiérrez, Alberto

doi:10.1016/j.lwt.2016.06.049

Cited by 123 publications

(98 citation statements)

References 31 publications

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“…Compound 1 was found at 2.47 min and m/z 443.1924 with a highly abundant fragment ion at m/z 101.0250. So this compound was tentatively identified as penstemide, the same as previously reported in avocado and Pistacia lentiscus L . leaves and it was also identified for the first time in citrus.…”

Section: Resultssupporting

confidence: 73%

“…leaves and it was also identified for the first time in citrus. Compound 2 ( t R = 2.78 min) with the [M–H] – ion at m/z 325.0934 was tentatively assigned as p ‐coumaric acid glucoside, which produced two characteristic ions at m/z 145.0298 ([M–H–Glc–H 2 O] − ) and 117.0349 ([M–H–Glc–H 2 O–CO] − ) , and this compound was first reported in citrus.…”

Section: Resultsmentioning

confidence: 99%

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Efficient analysis of phytochemical constituents in the peel of Chinese wild citrus Mangshanju (Citrus reticulata Blanco) by ultra high performance liquid chromatography–quadrupole time‐of‐flight‐mass spectrometry

Zhao

Xing

et al. 2018

J of Separation Science

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An efficient ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was developed for separation and profiling of phytochemical constituents of Chinese wild mandarin Mangshanju (Citrus reticulata Blanco). All constituents were well separated within 16 min. Based on retention times, accurate mass, MS fragments, and/or reference standards as well as databases, a total of 81 compounds were unambiguously identified or tentatively assigned including flavonoid glycosides, acylated flavonoid glycosides, flavones, polymethoxylated flavonoids, and limonoids as well as four other compounds. Among them, 22 polymethoxylated flavones and ten polymethoxylated flavanones/chalcones were identified in Mangshanju, more types than other citrus reported before. A basic procedure for identifying flavonoid-O-glycosides and the aglycones including polymethoxylated flavonoids was proposed. In addition, this method was successfully used to analyze another four mandarin germplasms, Cenxi suan ju, Xipi gousi gan, Nanfeng miju, and Or, showing that Mangshanju contained two characteristic compounds distinct from the other four citrus species. This study systematically profiled phytochemical constituents of Mangshanju, which was helpful for further utilization of Mangshanju owing to its abundant bioactive compounds.

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Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Efficient analysis of phytochemical constituents in the peel of Chinese wild citrus Mangshanju (Citrus reticulata Blanco) by ultra high performance liquid chromatography–quadrupole time‐of‐flight‐mass spectrometry

Zhao

Xing

et al. 2018

J of Separation Science

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“…Based on the MS and MS/MS data, the peaks 1 – 11 were identified as vanillic acid‐4‐ O ‐glucopyranoside, 2,5‐di‐hydroxybenzoic acid glucopyranoside, sinapic acid glucopyranoside, 1‐caffeoylquinic acid, kaempferol‐3,4′‐ O ‐di‐glucopyranoside, quercetin‐3,7‐ O ‐di‐glucopyranoside, naringenin‐3‐ O ‐glucopyranosyl‐rhamnoside, daidzein, biochanin A‐7‐ O ‐glucopyranoside, pratensein, and biochanin A, respectively. The data shown in Table are consistent with findings of previous studies .…”

Section: Resultssupporting

confidence: 92%

In vitro screening and isolation of human aromatase inhibitors from Cicer arietinum by a novel continuous online method combining chromatographic techniques

Zhang

Liu

et al. 2017

J of Separation Science

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Ultrafiltration liquid chromatography with mass spectrometry can efficiently and rapidly screen and identify ligands from the seeds of Cicer arietinum for human aromatase. Using this method, we identified 11 major compounds, including organic acids, organic acid glycosides, flavone glycosides, isoflavones, and isoflavone glycosides, as potent human aromatase inhibitors. A continuous online method, including pressurized liquid extraction, countercurrent chromatography, and preparative liquid chromatography, was developed for scaling up the production of these compounds with high purity and efficiency. The bioactivity of the separated compounds was assessed by an in vitro enzyme inhibition assay. This novel approach using a combination of ultrafiltration liquid chromatography with mass spectrometry and pressurized liquid extraction with countercurrent chromatography and preparative liquid chromatography as well as an in vitro enzyme inhibition assay could be applied to efficiently screen and isolate human aromatase inhibitors from complex samples and to the large-scale production of functional food and nutraceutical ingredients.

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“…There are few avocado by-product studies in literature, and the existing articles report works with non-compatible solvents allowed by the food and pharmaceutical authorities (FDA, 2017), such as methanol or hexane (Kosińska et al, 2012;López-Cobo et al, 2016;Rodríguez-Carpena et al, 2011), hindering the use of their final extracts. For this reason, we decided to performed a complete research based on an efficient food grade solvent, working on the hydrophilic and lipophilic antioxidant profile, a deep characterization of the bioactive compounds through HPLC-DAD-ESI/MS was performed, a wider antimicrobial analysis employing more pathogenic strains than those already tested by other authors and adding up the cytotoxic potential assays, to finally compile all this information in order to acquire better understanding of their intrinsic compounds and functions, which would allow to find an adequate use of these by-products as an alternative natural ingredient.…”

Section: Peak Tentative Identificationmentioning

confidence: 99%

Bioactive characterization of Persea americana Mill. by-products: A rich source of inherent antioxidants

Melgar

Días

Ćirić

et al. 2018

Industrial Crops and Products

128

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A B S T R A C TAvocado (Persea americana Mill.) is a worldwide consumed fruit, with great interest for cosmetic and pharmaceutical industries; however, 30% of avocado fruits are bio-wastes (peels and kernels), converting them into a potential source of bioactive compounds, such as phenolic compounds. Therefore, the hydroethanolic extracts of peels and kernels of Persea america Mill. var. Hass were analysed regarding their individual phenolic profile by HPLC-DAD/ESI-MS and correlated with their antioxidant, antimicrobial and cytotoxic activities. Avocado byproducts presented a very distinct phenolic profile, presenting higher concentration in peels (227.9 mg/g of extract for total phenolic content), mainly in (epi)catechin derivatives (175 mg/g of extract), followed by chlorogenic derivatives (42.9 mg/g of extract). In this study hydrophilic and lipophilic antioxidant assays were performed together for the first time in P. americana by-products, and although kernels showed a great antioxidant potential (EC 50 values ranging from 18.1 to 276 μg/mL), peels presented the highest potential (EC 50 ranging from 11.7 to 152 μg/mL), mainly due to the presence of phenolic compounds, and an overall better performance in the antibacterial assays. Further studies needs to be conducted to better understand the correlation between the presence of phenolic compounds and bioactivities, however, the main objective is to implement these biocompounds in different products and industries, due to results obtained, P. americana peels could be a great alternative in the substitution of synthetic antioxidants.

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HPLC-DAD-ESI-QTOF-MS and HPLC-FLD-MS as valuable tools for the determination of phenolic and other polar compounds in the edible part and by-products of avocado

Cited by 123 publications

References 31 publications

Efficient analysis of phytochemical constituents in the peel of Chinese wild citrus Mangshanju (Citrus reticulata Blanco) by ultra high performance liquid chromatography–quadrupole time‐of‐flight‐mass spectrometry

Efficient analysis of phytochemical constituents in the peel of Chinese wild citrus Mangshanju (Citrus reticulata Blanco) by ultra high performance liquid chromatography–quadrupole time‐of‐flight‐mass spectrometry

In vitro screening and isolation of human aromatase inhibitors from Cicer arietinum by a novel continuous online method combining chromatographic techniques

Bioactive characterization of Persea americana Mill. by-products: A rich source of inherent antioxidants

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