HPLC Determination of Etoposide in Injectable Dosage Forms

Abstract

1.The present study describes a direct and an alternative HPLC method for the determination of etoposide in injectable dosage forms. Etoposide, an antineoplastic drug, was eluted through C 18 ODS Hypersil column of 250 × 4.6 mm id with 5 µm particle size using simple mobile phase of methanol: water (1:1, v/v) at a flow rate of 1.0 mL min -1 and detected at 204 nm. The limit of detection of etoposide was 0.03 µg mL -1. The proposed method was found linear in the range of 0.25 µg mL -1 to 62.5 µg mL -1 and CV was 1.3% at 0.25 µg mL -1 , 0.48% at 3.90 µg mL -1 and 0.13% at 62.5 µg mL -1 . The accuracy of the developed method for two injectable dosage forms was 99.4 and 99.7% with RSD of 0.3%. The validation results and statistical data demonstrate that the method is simple, cost effective, reproducible and suitable in quality analysis of the active ingredient and dosage form.