HPLC for Separation and Quantification of Deoxyribonucleic Acid Fragments and Measurement of Deoxyribonucleic Acid Degradation

Abstract

© Springer-Verlag Berlin Heidelberg 2014 urement of peak area repeatability. The recovery of approximately 1 ng μL −1 of a specific DNA spiked in a mixed DNA sample was 99.9 ± 3.6 %. The method was able to measure the degradation rate of 600 bp DNA with a variation of approximately 1 %.

Nucleic acid degradation as barrier to gene delivery: a guide to understand and overcome nuclease activity

“…High-performance liquid chromatography (HPLC) has been widely used in the separation, purification and quantification of nucleic acids owing to the high sensitivity, speed, reliability, feasibility and minimal sample size. [378][379][380] The sample in the mobile phase is pushed through a column (the stationary phase) by applying a high pressure. By interacting with the column, each component in the mobile phase will display a different flow rate, thus leading to the separation (Fig.…”