HPLC screening of anti-cancer sulforaphane from important European Brassica species

Sivakumar, G.; Aliboni, Andrea; Bacchetta, Loretta

doi:10.1016/j.foodchem.2006.11.040

Cited by 53 publications

(38 citation statements)

References 17 publications

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“…The results were expressed in mg per kg DM (dry matter). The sulforaphane content was determined by high liquid pressure chromatography (HPLC) method according to Sivakumar et al (2007) and presented as mg per kg DM. Total polyphenols were determined photometrically by the method of Lachman et al (2003) and expressed as mg of gallic acid equivalent (GAE) per kg DM.…”

Section: Methodsmentioning

confidence: 99%

Effect of zinc fertilisation on yield and selected qualitative parameters of broccoli

Šlosár¹,

Mezeyová²,

Hegedüsová³

et al. 2017

PLANT SOIL ENVIRON

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Three treatments were used in two-year (2014–2015) field experiments with broccoli cv. Bejo 2914 F1: (1) untreated control; (2) Zn0.75 – Zinkuran SC as foliar fertiliser at the rate of 0.75 L/ha (375 g Zn/ha); (3) Zn1.50 – Zinkuran SC as foliar fertiliser at the rate of 1.50 L/ha (750 g Zn/ha). The statistically significant differences of individual broccoli parameters were found after zinc applications. In both experimental years the yield of broccoli with additional zinc fertilisation was significantly higher by about 8.2–14.4% (Zn0.75) and 12.5–17.5% (Zn1.50), respectively, than in the control. Foliar zinc application significantly increased the sulforaphane content in broccoli florets by about 19.8–32.9% (Zn0.75) and 37.2–49.3% (Zn1.50), respectively, compared to the control. By contrast, the content of total polyphenolics (of about 9.0–12.5% (Zn0.75) and 33.9–35.2% (Zn1.50)) and antioxidant activity (Zn0.75 (3.7–4.2%) and Zn1.50 (5.3–7.0)) decreased as a result of zinc fertilisation. The investigations pointed to zinc as a very important micronutrient with strong influence on the yield and chosen qualitative, health promoting parameters of broccoli.

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Section: Methodsmentioning

confidence: 99%

Effect of zinc fertilisation on yield and selected qualitative parameters of broccoli

Šlosár¹,

Mezeyová²,

Hegedüsová³

et al. 2017

PLANT SOIL ENVIRON

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“…It is very interesting that there were significant differences, 1.5-300 times, among the broccoli tissues that can be comparable with the other results. [15][16][17] The highest content of sulforaphane was found in broccoli 7-day-seedling, whereas leaves recorded the lowest. However, differences in sulforaphane contents between the 7-day-old seedling and seed were relatively slight.…”

Section: Linearity Of Standard Precision and Recoverymentioning

confidence: 99%

“…Some authors, previously, were investigated amounts of sulforaphane in broccoli seedling, 16 seed, 15 stalk and leave 17 and package production. 19 While, this study investigate broccoli sulforaphane from seed until mature plant.…”

Section: Subheadingmentioning

confidence: 99%

“…HPLC A large number of previous studies for separation of sulforaphane in real samples have used C 18 analytical column and linear gradient elution by mixture of water-acetonitrile 15,17 or some special isocratic methods by different mobile phases comprising mixture of water-acetonitrile 16 or water/tetrahydrofuran; 14 while in this study a C 8 analytical column and mixture of water/acetonitrile 65/35 (v/v) as mobile phase was used. Although, newly introduced HPLC-ELSD 19 method to determine broccoli sulforaphane with high selectivity and sensitivity.…”

Section: Subheadingmentioning

confidence: 99%

“…This was also noted by other workers. 9,16 This change may be related to several different myrosinase isoenzymes have been characterized in seeds, seedlings, and vegetative tissues. 23 Thus, the source of myrosinase activity should be carefully considered when studying the products derived from glucosinolate hydrolysis.…”

Section: Linearity Of Standard Precision and Recoverymentioning

confidence: 99%

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The Isolation and Determination of Sulforaphane from Broccoli Tissues by Reverse Phase‐High Performance Liquid Chromatography

Azizi

Amiri-Besheli

Sharifi-Mehr

2011

J Chinese Chemical Soc

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Sulforaphane is a cognate isothiocyanate of glucoraphanin, released by degradation of glucosinolate through myrosinase enzyme, when Brassica tissues are crushed or chewed. C 8 analytical column and mixture of water/acetonitrile 65/35 (v/v) as mobile phase were used to separate sulforaphane in broccoli tissues (e.g., 7-day-seedling, seed, floret and leave). Confirmation of sulforaphane in crude extract of broccoli tissues was monitored using gas chromatography-mass spectroscopy (GC-MS). The highest amount of sulforaphane (1216 µg/g dry weight) found in 7-day-seedling. This method is suitable for routine screening of plant materials and proposes a low cost and robust technique for the analysis of sulforaphane.

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HPLC method validation for measurement of sulforaphane level in broccoli by‐products

Campas‐Baypoli

Sánchez-Machado

Bueno-Solano

et al. 2009

Biomedical Chromatography

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A simple and specific analytical method was developed and tested for the determination of sulforaphane in broccoli by-products. The method includes the optimization of the conversion of glucoraphanin to sulforaphane, followed by purification of extracts using solid-phase extraction and high-performance liquid chromatography (HPLC) analysis. The response surface methodology was used to find optimum conditions for the preparation and purification procedure. Chromatographic conditions for reversed-phase HPLC with UV photodiode array detection were as follows: column, Exil ODS C(18), 25 x 0.46 cm, 5 microm; column temperature, 36 degrees C; mobile phase, a 30 : 70 (v/v) mixture of acetonitrile:water; flow rate, 0.6 mL/min. The detection wavelength was UV 202 nm. Under these conditions, excellent linearity was obtained (r(2) = 1), and the overall recovery was 97.5 and 98.1% for fresh florets and lyophilized florets, respectively. The precision results showed that the relative standard deviation of the repeatability for florets fresh and lyophilized was 3.0 and 4.0%, respectively. Sulforaphane contents were determined in the edible portion of fresh broccoli, and broccoli crop remains.

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HPLC screening of anti-cancer sulforaphane from important European Brassica species

Cited by 53 publications

References 17 publications

Effect of zinc fertilisation on yield and selected qualitative parameters of broccoli

Effect of zinc fertilisation on yield and selected qualitative parameters of broccoli

The Isolation and Determination of Sulforaphane from Broccoli Tissues by Reverse Phase‐High Performance Liquid Chromatography

HPLC method validation for measurement of sulforaphane level in broccoli by‐products

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