HPV Types Distribution in General Female Population and in Women Diagnosed with Cervical Cancer Across Western Kazakhstan

Balmagambetova, Saule; Tinelli, Andrea; Urazayev, Olzhas; Sakieva, Kanshaiym Z; Koyshybaev, Arip; Zholmukhamedova, Dinara; Urazayeva, Saltanat

doi:10.31557/apjcp.2019.20.4.1089

Cited by 5 publications

(6 citation statements)

References 21 publications

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“…One follow-up study on cervical cases classified as ASCUS and negative for high-risk HPV found that, one year later, 23% had progressed to LSIL and 4% to HSIL (Ozturk et al, 2016). Furthermore, Balmagambetova et al, (2019), in a review of the literature, noted the reported presence of low-risk HPV types 6 and 11 in invasive anal cancer cases (Balmagambetova et al, 2019) and Serrano et al, (2015) reported low-risk HPV associated with cancers of the anus, cervix, vagina and vulva (Serrano et al, 2015). Several previous studies have reported p16/Ki-67 positivity is independent of HPV genotypes and achieved a sensitivity equal to HPV testing with significantly higher specificity (Zhang et al, 2019;Hu et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Human Papillomavirus Detection and Abnormal Anal Cytology in HIV-infected Patients Using p16/Ki-67 Dual-Staining

Patarapadungkit

Khonhan

Pisuttimarn

et al. 2020

Asian Pac J Cancer Prev

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Objective: We investigated human papillomavirus (HPV) infection and detected anal squamous intraepithelial lesions by modified liquid-based cytology (LBC) and p16/Ki67 dual-staining. Methods: Anal swabs (n=393) were collected from patients with HIV infection. Anal cells were kept in 95% ethyl alcohol for modified LBC. DNA was extracted from cells for HPV detection and genotyping using real-time PCR and reverse line blot hybridization. Results: Nine samples (2.3%) were unsatisfactory specimens, 74.8% (294/393) were negative for intraepithelial malignancies (NILM) and 22.9% (90/393) exhibited squamous intraepithelial lesions (SIL). In the latter category, 13.7% of samples (54/393) contained atypical squamous cells of undetermined significance (ASCUS), 6.9% (27/393) were classified as low-grade SIL (LSIL) and 2.3% (9/393) as high-grade SIL (HSIL). A total of 331 from 393 swab samples were suitable for detection of HPV infection. Among these, 34.1% (113/331) were positive. HPV 58 (15.9%) was the most common genotype, followed by HPV 18 (14.2%) and HPV 16 (11.5%). The severity of abnormal cells was significantly associated with HPV infection. Dual staining with p16/Ki-67 was performed on 130 samples: in 30.8% (40/130) of samples positive staining was significantly associated with severity of abnormal cells. Agreement between cytology, p16/Ki67 dual-staining and high-risk HPV detection was 100% in HSIL samples. Interestingly, eight apparently NIML cases might have contained abnormal cells, since they were positive by both p16/Ki67 dual-staining and high-risk HPV detection. Conclusion: Anal specimens screened using modified LBC with 95% ethyl alcohol solution as the fixative are suitable for screening anal precancerous lesions by cytology, HPV testing and p16/Ki-67 dual staining.

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Section: Discussionmentioning

confidence: 99%

Human Papillomavirus Detection and Abnormal Anal Cytology in HIV-infected Patients Using p16/Ki-67 Dual-Staining

Patarapadungkit

Khonhan

Pisuttimarn

et al. 2020

Asian Pac J Cancer Prev

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“…Test systems "Quantum-21" (Russian manufacturing) were used for qualitative and quantitative detection of total HPV 21 types (6,11,16,18,26,31,33,35,39,44,45,51,52,53,56,58,59, 66, 68, 73, and 82). Of them, thirteen are highly carcinogenic, five are possibly/probably carcinogenic (26,53,66,73,82), and three are low carcinogenic types (6,11,44). DNA extraction for further L1 gene sequencing was performed with QIAamp DNA Mini Kit (Qiagen Ltd, Crawley, UK).…”

Section: Methodsmentioning

confidence: 99%

“…Their findings were then widely cited in international HPV bulletins [8][9][10]. However, more recent authors reported higher rates of HPV infection in various regions of the country, within 25% [11] and even higher. Among Kazakhstani women attending the gynecologic clinics, high-risk HPV was found in 39%, and 13% were infected with multiple HR-HPV types [12].…”

Section: Introductionmentioning

confidence: 99%

“…Among Kazakhstani women attending the gynecologic clinics, high-risk HPV was found in 39%, and 13% were infected with multiple HR-HPV types [12]. All homeland researchers report the predominance of the most carcinogenic HPV type 16 in the swabs of Kazakhstani women [11][12][13][14]. In the five Central Asian countries that were formerly part of the Soviet Union (Kazakhstan, Kyrgyzstan, Tajikistan, Turkmenistan, and Uzbekistan), cervical cancer incidence and mortality rates are far higher than those in most Western and high-income nations and are increasing [15].…”

Section: Introductionmentioning

confidence: 99%

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High-risk human papillomaviruses l1 gene isolates identified in Western Kazakhstan

Balmagambetova,

Zholdybayeva,

Zavalennaya

et al. 2024

J CLIN MED KAZ

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Kazakhstani researchers reported a significant prevalence of highly carcinogenic human papillomavirus types in the country. The article aimed to present HPV L1 gene sequencing developments in women affected with cervical cancer throughout the western part of Kazakhstan with provided findings on the geographic pathways of obtained isolates. Methods. The HPV L1 gene was amplified using the consensus primers MY09HPV 5’-CGTCCMARRGGAWACTGATC-3’ and MY11HPV 5’ – GCMCAGGGWCATAAYAATGG-3’.  The purified DNA was used as the target for direct nucleotide sequencing. Phylogenetic analyses were conducted using the MegAlign program from the LASERGENE software package (version 6.0; DNA star, Madison, WI) and with MEGA version 5.0 software. A multiple alignment was created through Clustal W software, and the neighbor-joining method was used to construct the phylogenetic tree. Results. Of 70 HPV samples transported to the Astana shared laboratory for gene L1 sequencing, only ten appeared fit to obtain isolates (14.3%). The viral load of the samples ranged from 3.3 to 8.2, and the range of DNA concentration was from 8.16 to 69.6 ng/uL. HPV16 unique Kazakhstani isolate from Aktobe, having its own branch, and not yet registered in the world genebank, was revealed. An isolate of potentially carcinogenic HPV53 forming a remote cluster with KF436822/1, KU951264.1 - Southwest China, and 97% identity with EU056643.1 - Ireland, and acted as a single agent for invasive cervical cancer was identified. In general, the sequencing findings indicate the variety of ways for HPV pervasion into the western region of Kazakhstan: North and South America, Europe, and Asia. The study was recorded in the ISRCTN registry, No. 7154910, 02/01/2018.

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“…Cancer Statistics Working Group, 2020). Some studies on this topic have been carried out in Kazakhstan (Igissinov et al, 2012;Aimagambetova and Azizan, 2018;Balmagambetova et al, 2019). However, the problem remains relevant and interesting from an epidemiological point of view.…”

Section: New Trends Of Cervical Cancer Incidence In Kazakhstanmentioning

confidence: 99%

New Trends of Cervical Cancer Incidence in Kazakhstan

Igissinov

Igissinova

Telmanova

et al. 2021

Asian Pac J Cancer Prev

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Objective: The epidemiological features of the cervical cancer (CC) incidence and its spatial and temporal assessment in Kazakhstan were studied. Methods: The retrospective study was done for the period 2009-2018. Descriptive and analytical methods of oncoepidemiology were used. Results: During the study period, 16,441 new cases of CC were registered. The average annual crude and age-standardized incidence rate were 18.6±0.5 and 17.7±0.4 cases per 100,000 population of female, respectively, and their trends tended to increase (Т up =+2.3%; R 2 =0.708 and Т up =+1.9%; R 2 =0.615, respectively). The analysis of ASIR showed unimodal growth with a peak at 50-54 years -45.3±1.1 cases per 100,000 population of female. Trends of ASIR decreased up to 30 years (T down =−1.8%; R 2 =0.111) and 35-59 years (T down =−0.9%; R 2 =0.103), in other age groups the trends increased, and were most pronounced in 40-44 (Т up =+4.1%; R 2 =0.878) and 65-69 years (Т up =+4.4%; R 2 =0.537). Trends in ASR of СС tended to grow in almost all regions, with higher levels in Mangystau (Т up =+4.1%; R 2 =0.482) and Aktobe (Т up =+6.3%; R 2 =0.846) regions. The cartograms of ASR per 100,000 population of female were allocated according to the following criteria: low -up to 16.3, averagefrom 16.3 to 19.2, high -above 19.2. The results of the spatial analysis showed the regions with a higher levels of CC incidence rate per 100,000 population of female: East Kazakhstan (19.8), Aktobe (20.0), Almaty (20.1), Kostanay region (20.9), Atyrau (21.7) regions and Almaty city (22.0). Conclusion: The study of trends of the cervical cancer incidence has the theoretical and practical value: monitoring and evaluation of screening programmes, which are implemented in the country, and conduction of secondary prevention of cervical pathology. Health authorities should consider the obtained results in the in the organization of anti-cancer activities.

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HPV Types Distribution in General Female Population and in Women Diagnosed with Cervical Cancer Across Western Kazakhstan

Cited by 5 publications

References 21 publications

Human Papillomavirus Detection and Abnormal Anal Cytology in HIV-infected Patients Using p16/Ki-67 Dual-Staining

Human Papillomavirus Detection and Abnormal Anal Cytology in HIV-infected Patients Using p16/Ki-67 Dual-Staining

High-risk human papillomaviruses l1 gene isolates identified in Western Kazakhstan

New Trends of Cervical Cancer Incidence in Kazakhstan

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