HPV Viral Activity by mRNA-HPV Molecular Analysis to Screen the Transforming Infections in Precancer Cervical Lesions

Tinelli, Andrea; Leo, Giuseppe; Pisanò, Maurizio; Storelli, Fabio; Leo, Sara; Vergara, Daniele; Malvasi, Antonio

doi:10.2174/138920109789978739

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…Therefore, evaluation of E6 and E7 mRNA in high risk genotypes may represent a good marker for HPV involvement in malignant transformation. In fact, a strong association between high risk mRNA-HPV presence and risk of neoplastic progression in cervical lesions has been described [34], [35]. In consequence, E6 and E7 mRNA-HPV presence in high risk phenotypes was suggested as a tool for early identification of cervical lesions that would evolve toward neoplastic transformation.…”

Section: Resultsmentioning

confidence: 99%

Presence of Human Papilloma Virus in a Series of Breast Carcinoma from Argentina

et al. 2013

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BackgroundThe etiology and the molecular mechanisms related to breast carcinogenesis remain poorly understood. Some recent reports have examined the role of Human Papillomavirus (HPV) in this disease. The purpose of this study was to determine the prevalence of HPV in breast cancer.MethodsSixty one fresh frozen breast cancers samples were analyzed. Samples were tested for HPV by PCR, and products were automatically sequenced. Findings were correlated with clinical and pathological characteristics.ResultsThe HPV DNA prevalence in the breast cancer samples was 26% (16/61). Clinical parameters were not statistically associated with HPV presence (p>0.05 χ2 test). Sequence analysis in a subgroup of cases indicates the prevalence of low risk HPV11, followed by high risk HPV16. We found no HPV transcriptional activity.ConclusionThe present study demonstrated for the first time in Argentina the presence of HPV in a proportion of the malignant breast tissues. This finding suggests that HPV may have a biological significance in breast carcinogenesis.

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Section: Resultsmentioning

confidence: 99%

Presence of Human Papilloma Virus in a Series of Breast Carcinoma from Argentina

et al. 2013

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“…Negative control reactions, consisting of all reagents except RNA, were performed at each run. mRNA testing was defined as positive if at least one of the five HPV genotypes detected by the test has been found [11]. …”

Section: Methodsmentioning

confidence: 99%

Chromogenic In Situ Hybridization and p16/Ki67 Dual Staining on Formalin-Fixed Paraffin-Embedded Cervical Specimens: Correlation with HPV-DNA Test, E6/E7 mRNA Test, and Potential Clinical Applications

Zappacosta

Colasante

Viola

et al. 2013

BioMed Research International

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Although HPV-DNA test and E6/E7 mRNA analyses remain the current standard for the confirmation of human papillomavirus (HPV) infections in cytological specimens, no universally adopted techniques exist for the detection of HPV in formalin-fixed paraffin-embedded samples. Particularly, in routine laboratories, molecular assays are still time-consuming and would require a high level of expertise. In this study, we investigated the possible use of a novel HPV tyramide-based chromogenic in situ hybridization (CISH) technology to locate HPV on tissue specimens. Then, we evaluate the potential usefulness of p16INK4a/Ki-67 double stain on histological samples, to identify cervical cells expressing HPV E6/E7 oncogenes. In our series, CISH showed a clear signal in 95.2% of the specimens and reached a sensitivity of 86.5%. CISH positivity always matched with HPV-DNA positivity, while 100% of cases with punctated signal joined with cervical intraepithelial neoplasia grade 2 or worse (CIN2+). p16/Ki67 immunohistochemistry gave an interpretable result in 100% of the cases. The use of dual stain significantly increased the agreement between pathologists, which reached 100%. Concordance between dual stain and E6/E7 mRNA test was 89%. In our series, both CISH and p16INK4a/Ki67 dual stain demonstrated high grade of performances. In particular, CISH would help to distinguish episomal from integrated HPV, in order to allow conclusions regarding the prognosis of the lesion, while p16INK4a/Ki67 dual stain approach would confer a high level of standardization to the diagnostic procedure.

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“…Infectiousness may depend on HPV viral load and this, in turn, may depend on the stage of infection. It has been suggested that the detection of mRNA may be a better indicator of a productive viral infection, and several studies have been conducted to investigate the prognostic value of this test [33][34][35][36][37][38][39] but as yet, no large-scale study has been conducted to establish a correlation between the presence of mRNA transcripts or viral load with transmissibility.…”

Section: How Do We Interpret Dna Positivity?mentioning

confidence: 99%

Unresolved questions concerning human papillomavirus infection and transmission: a modelling perspective

2010

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Abstract. Mathematical transmission models are widely used to forecast the potential impact of interventions such as vaccination and to inform the development of health policy. Effective vaccines are now available for the prevention of cervical cancer and other diseases attributable to human papillomavirus (HPV). Considerable uncertainties remain regarding the characterisation of HPV infection and its sequelae, infectivity, and both vaccine-conferred and naturallyacquired immunity. In this review, we discuss the key knowledge gaps that impact on our ability to develop accurate models of HPV transmission and vaccination.

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HPV Viral Activity by mRNA-HPV Molecular Analysis to Screen the Transforming Infections in Precancer Cervical Lesions

Abstract: the results suggest the potential of E6/E7 detection to target the presence of a transforming HPV infection.

Cited by 6 publications

References 16 publications

Presence of Human Papilloma Virus in a Series of Breast Carcinoma from Argentina

Presence of Human Papilloma Virus in a Series of Breast Carcinoma from Argentina

Chromogenic In Situ Hybridization and p16/Ki67 Dual Staining on Formalin-Fixed Paraffin-Embedded Cervical Specimens: Correlation with HPV-DNA Test, E6/E7 mRNA Test, and Potential Clinical Applications

Unresolved questions concerning human papillomavirus infection and transmission: a modelling perspective

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