Hrs1p/Mcp6p on the Meiotic SPB Organizes Astral Microtubule Arrays for Oscillatory Nuclear Movement

Tanaka, Kayoko; Kohda, Toshiki A.; Yamashita, Akira; Nonaka, Nobuhiro; Yamamoto, Masayuki

doi:10.1016/j.cub.2005.07.058

Cited by 41 publications

(51 citation statements)

References 29 publications

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“…Hrs1 (also called Mcp6) is a meiosisspecific component of the SPB, and hrs1Δ mutant cells show defects in nuclear movement (Fig. 3A, B) as previously reported (Tanaka et al, 2005;Saito et al, 2005). Cells of the dhc1Δ (dynein heavy chain) mutant also show defective nuclear movement (Fig.…”

Section: Spindle Defects In Bqt2δ Zygotes Are Rescued By Stopping Nucsupporting

confidence: 84%

Chromosomes Rein Back the Spindle Pole Body during Horsetail Movement in Fission Yeast Meiosis

Chikashige

Yamane

Okamasa

et al. 2014

Cell Struct. Funct.

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ABSTRACT. In meiosis, pairing and recombination of homologous chromosomes are crucial for the correct segregation of chromosomes, and substantial movements of chromosomes are required to achieve homolog pairing. During this process, it is known that telomeres cluster to form a bouquet arrangement of chromosomes. The fission yeast Schizosaccharomyces pombe provides a striking example of bouquet formation, after which the entire nucleus oscillates between the cell poles (these oscillations are generally called horsetail nuclear movements) while the telomeres remain clustered to the spindle pole body (SPB; a centrosome-equivalent structure in fungi) at the leading edge of the moving nucleus. S. pombe mutants defective in telomere clustering frequently form aberrant spindles, such as monopolar or nonpolar spindles, leading to missegregation of the chromosomes at the subsequent meiotic divisions. Here we demonstrate that such defects in meiotic spindle formation caused by loss of meiotic telomere clustering are rescued when nuclear movement is prevented. On the other hand, stopping nuclear movement does not rescue defects in telomere clustering, nor chromosome missgregation even in cells that have formed a bipolar spindle. These results suggest that movement of the SPB without attachment of telomeres leads to the formation of aberrant spindles, but that recovering bipolar spindles is not sufficient for rescue of chromosome missegregation in mutants lacking telomere clustering.

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Section: Spindle Defects In Bqt2δ Zygotes Are Rescued By Stopping Nucsupporting

confidence: 84%

Chromosomes Rein Back the Spindle Pole Body during Horsetail Movement in Fission Yeast Meiosis

Chikashige

Yamane

Okamasa

et al. 2014

Cell Struct. Funct.

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“…Like Bqt2, Mcp6 is a meiosisspecific SPB protein in S. pombe. In mcp6D mutant meioses telomere clustering appears normal but horsetail nuclear movement is reduced (Saito et al 2005;Tanaka et al 2005). In contrast to our view above, the frequency of ectopic recombinants is increased modestly in mcp6D mutant meioses (Saito et al 2005).…”

Section: Discussioncontrasting

confidence: 98%

The Meiotic Bouquet Promotes Homolog Interactions and Restricts Ectopic Recombination inSchizosaccharomyces pombe

Davis

Smith

2006

Genetics

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Chromosome architecture undergoes extensive, programmed changes as cells enter meiosis. A highly conserved change is the clustering of telomeres at the nuclear periphery to form the ''bouquet'' configuration. In the fission yeast Schizosaccharomyces pombe the bouquet and associated nuclear movement facilitate initial interactions between homologs. We show that Bqt2, a meiosis-specific protein required for bouquet formation, is required for wild-type levels of homolog pairing and meiotic allelic recombination. Both gene conversion and crossing over are reduced and exhibit negative interference in bqt2D mutants, reflecting reduced homolog pairing. While both the bouquet and nuclear movement promote pairing, only the bouquet restricts ectopic recombination (that between dispersed repetitive DNA). We discuss mechanisms by which the bouquet may prevent deleterious translocations by restricting ectopic recombination. M EIOSIS, the specialized form of nuclear division that reduces the diploid number of chromosomes by half, consists of one round of DNA replication followed by two successive nuclear divisions. At the first meiotic division (MI) homologous chromosomes (homologs), each consisting of two sister chromatids, are segregated to opposite poles, reducing the chromosome number by half. The second meiotic division (MII), like mitosis, segregates sister chromosomes to opposite poles, producing four haploid nuclei.

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“…6B), a meiosis-specific protein that is required for astral microtubule positioning to maintain horsetail movement in meiosis I. 38,39 Determination of the phosphorylation sites of Rhp54 and Mcp6 and other Mek1 association partners, and of their physiological significance, is the target of future experiments.…”

Section: Discussionmentioning

confidence: 99%

The Mek1 phosphorylation cascade plays a role in meiotic recombination ofSchizosaccharomyces pombe

et al. 2010

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Hrs1p/Mcp6p on the Meiotic SPB Organizes Astral Microtubule Arrays for Oscillatory Nuclear Movement

Cited by 41 publications

References 29 publications

Chromosomes Rein Back the Spindle Pole Body during Horsetail Movement in Fission Yeast Meiosis

Chromosomes Rein Back the Spindle Pole Body during Horsetail Movement in Fission Yeast Meiosis

The Meiotic Bouquet Promotes Homolog Interactions and Restricts Ectopic Recombination inSchizosaccharomyces pombe

The Mek1 phosphorylation cascade plays a role in meiotic recombination ofSchizosaccharomyces pombe

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