Hsa_circ_0076305 induces migration-proliferation dichotomy in gastric cancer

Abstract

Recent studies have demonstrated that circular RNAs (circRNAs) play an important role in the development of gastric cancer (GC). The present study aimed to investigate the role of hsa_circ_0076305 (circPGC) in GC. The levels of circRNAs and mRNAs in AGS cell lines were detected via reverse transcription-quantitative PCR, and western blotting was performed to detect protein expression levels. Functional studies were explored by CCK8 assay and cell migration assay. Functional studies have indicated that circPGC orchestrates two cellular processes; it inhibits proliferation, and promotes migration and invasion in the GC AGS cell line, a phenomenon called ‘migration-proliferation dichotomy’, as well as epithelial-to-mesenchymal transition in AGS cells. In addition, circPGC degrades the extracellular matrix and basement membrane through matrix metallopeptidase (MMP)9 and MMP14, providing a microenvironment that facilitates cell migration. The results also demonstrated that circPGC expression is lower in clinical patients with later stages of GC, which is associated with poor prognosis. Taken together, these results suggest that circPGC exhibits migration-proliferation dichotomy during GC development, invasion and migration.

Circ_0076305 facilitates prostate cancer development via sponging miR‐411‐5p and regulating PGK1

“…As for circ_0076305, it was upregulated in lung cancer and elevated the cisplatin resistance of lung cancer cells (Dong et al, 2019). In addition, circ_0076305 was low‐expressed in gastric cancer and induced migration‐proliferation dichotomy in gastric cancer (Zhang, Hong, et al, 2021). More importantly, an apparent upregulation of circ_0076305 expression was detected in PCa tissue specimens (Ge et al, 2020).…”

“…Moreover, miR‐411‐5p decreased GRB2 to limit breast cancer cell metastasis and proliferation (Zhang et al, 2016). By contrast, miR‐411‐5p is able to serve as a tumour accelerator in neck squamous cell carcinoma (Zhang et al, 2021). In PCa, miR‐411‐5p was downregulated miRNA and miR‐411‐5p inhibited PCa progression (Pan et al, 2021).…”