Latar Belakang: Kekurangan nutrisi selama seribu hari pertama kelahiran dapat menyebabkan terjadinya stunting. Kondisi ini dapat mempengaruhi perkembangan fisik terganggu, penurunan kognitif, motorik dan performa kerja anak menurun. Sanitasi lingkungan yang buruk merupakan faktor tidak langsung yang dapat meningkatkan kemungkinan stunting. Kondisi lingkungan yang tidak sehat sebagai pemicu penyakit yang akhirnya dapat menurunkan status gizi balita. Tujuan penelitian untuk menganalisis hubungan antara kesehatan lingkungan dengan kejadian stunting di Puskesmas Kalasan.Metode: Jenis rancangan penelitian desain observasional analitik dengan desain case control. Perbandingan yang digunakan adalah 1:1. Populasi penelitian adalah ibu dengan balita usia 12-59 bulan di Puskesmas Kalasan. Total sampling digunakan untuk mengambil sampel kasus. Sampel kontrol diambil dengan menggunakan simple random sampling. Instrumen penelitian berupa kuesioner dan data dianilisis menggunakan uji chi square.Hasil: Hasil menunjukkan tidak ada hubungan yang signifikan antara sumber air bersih (p value=1,000; OR=1,000 (CI 0,56-17,41), kualitas fisik air bersih (p value=1,000; OR=0,47 (CI 0,04-5,72), kepemilikan jamban (p value=1,000; OR=1,31 (CI 0,31-5,53), dan kebiasaan cuci tangan (p value=1,000; OR=1,000 (CI 0,20-4,88) dengan kejadian stunting.Simpulan: Tidak terdapat hubungan antara kesehatan lingkungan dengan kejadian stunting di Puskesmas Kalasan. ABSTRACT Title: Relationship Between Environmental Health And Stunting in The Kalasan Health Center, Sleman Regency.Background: Lack of nutrition during the first thousand days of birth can cause stunting. This condition can affect impaired physical development, decreased cognitive, motor and work performance of children. Poor environmental sanitation is one of the indirect factors that causes stunting. Unhealthy environmental conditions as a trigger for diseases that can ultimately reduce the nutritional status of toddlers. Aimed of this study was to determine the relationship between environmental health and the incidence of stunting at the Kalasan Health Center.Method: Case control design is used in this study. The comparison used is 1:1. The study population was mothers with toddlers aged 12-59 months at the Kalasan Health Center. Total sampling is used to take case samples. Control samples were taken using simple random sampling. The research instrument was a questionnaire and the data were analyzed using the chi square test..Result: The results showed that there was no significant relationship between clean water sources (p value=1,000; OR=1,000 (95% CI 0,56-17,41), physical quality of clean water (p value=1,000; OR=0,47 (95% CI 0,04-5,72), latrine ownership (p value=1,000; OR=1,31 (95% CI 0,31-5,53), and hand washing habits (p value=1,000; OR=1,000 (95% CI 0,20-4,88) with the incidence of stunting.Conclusion: There is no direct relationship between environmental health and stunting in the Kalasan Public Health Center Sleman Regency.