Human amniotic fluid-derived c-kit+ and c-kit− stem cells: growth characteristics and some differentiation potential capacities comparison

Bai, Jing; Wang, Yiru; Liu, Lifeng; Chen, Jie; Yang, Wen-Lan; Lian-ru, Gao; Wang, Yu

doi:10.1007/s10616-012-9441-6

Cited by 21 publications

(18 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A possible explanation is that CD117 + cells represent a very small percentage of the total huAFSC population and if not selected at the very early stages of cell culturing, they can be obscured to flow cytometric detection by the more abundant CD117 − cell population, which becomes more and more predominant passage after passage in culture. Noteworthy, regardless of being CD117 − , in our hands and in agreement with previous findings [52–54], huAFSC showed a good differentiation potential, even after a number of passages in culture. They were able to undergo, when grown in appropriate medium, osteogenic or adipogenic commitment, as documented by the upregulation of early molecular markers and the occurrence of phenotypic changes (Figs.…”

Section: Discussionsupporting

confidence: 93%

Wnt Signaling Behaves as a “Master Regulator” in the Osteogenic and Adipogenic Commitment of Human Amniotic Fluid Mesenchymal Stem Cells

D’Alimonte

Lannutti

Pipino

et al. 2013

Stem Cell Rev and Rep

View full text Add to dashboard Cite

Human amniotic fluid mesenchymal stem cells (huAFMSCs) are emerging as a promising therapeutic option in regenerative medicine. Here, we characterized huAFMSC phenotype and multipotentiality. When cultured in osteogenic medium, huAFMSC displayed a significant increase in: Alkaline Phosphatase (ALP) activity and mRNA expression, Alizarin Red S staining and Runx2 mRNA expression; whereas maintaining these cells in an adipogenic culture medium gave a time-dependent increase in PPARγ and FABP4 mRNA expression, glycerol-3-phosphate dehydrogenase (GPDH) activity and positivity to Oil Red Oil staining. These results confirm that huAFMSCs can differentiate toward osteogenic and adipogenic phenotypes. The canonical Wnt/ßcatenin signaling pathway appears to trigger huAFMSC osteoblastogenesis, since during early phases of osteogenic differentiation, the expression of Dishevelled-2 (Dvl-2), of the non-phosphorylated form of ß-catenin, and the phosphorylation of glycogen synthase kinase-3ß (GSK3ß) at serine 9 were upregulated. On the contrary, during adipogenic differentiation Dvl-2 expression decreased, whereas that of ß-catenin remained unchanged. This was associated with a late increase in GSK3ß phosphorylation. Consistent with this scenario, huAFMSCs exposure to Dickkopf-1, a selective inhibitor of the Wnt signaling, abolished Runx2 and ALP mRNA upregulation during huAFMSC osteogenic differentiation, whereas it enhanced FABP4 expression in adipocyte-differentiating cells. Taken together, these results unravel novel molecular determinants of huAFMSC commitment towards osteoblastogenesis, which may represent potential targets for directing the differentiation of these cells and improving their use in regenerative medicine.FigureSchematic representation of Wnt pathway involved in the osteogenic and adipogenic differentiation of huAFMSCs. Our paper demonstrates that osteogenic commitment of these cells is linked to the stimulation of Wnt signal leading to the final transcriptional activation of early osteogenic markers such as RUNX-2 and ALP, mediated by β-catenin. DKK1 is a secreted Wnt antagonist that may be used as a drug to inhibit Wnt signal. In contrast, adipogenic commitment involves early inhibition of Wnt pathway leading to ubiquitination/degradation of β-catenin. This results in the transcription of PPARγ and FABP4, considered as the main initiators of adipogenesis. APC, adenomatous polyposis coli; βcat, β-catenin; CK1, casein kinase 1; DKK1, dickkopf 1; Dvl, Dishevelled; GSK3β, glycogen synthase kinase 3β; LRP5/6, low density lipoprotein receptor-related protein 5/6

show abstract

Section: Discussionsupporting

confidence: 93%

Wnt Signaling Behaves as a “Master Regulator” in the Osteogenic and Adipogenic Commitment of Human Amniotic Fluid Mesenchymal Stem Cells

D’Alimonte

Lannutti

Pipino

et al. 2013

Stem Cell Rev and Rep

View full text Add to dashboard Cite

show abstract

“…In the present work, stem cell factor receptor c‐kit was expressed by only 1% of the af‐MSCs. Although most previous studies were performed with c‐kit‐positive‐sorted af‐MSCs, recent work showed that c‐kit‐positive and c‐kit‐negative af‐MSCs mostly share the same differentiation potential, except that for myocardial differentiation [25]. Consequently, we selected af‐MSCs by direct adhesion because this method is suitable for clinical application and avoids cell sorting, which is expensive and time consuming.…”

Section: Discussionmentioning

confidence: 99%

Amniotic Fluid-Derived Mesenchymal Stem Cells Prevent Fibrosis and Preserve Renal Function in a Preclinical Porcine Model of Kidney Transplantation

Baulier

Favreau

Corf

et al. 2014

Stem Cells Translational Medicine

View full text Add to dashboard Cite

It is well known that ischemia/reperfusion injuries strongly affect the success of human organ transplantation. Development of interstitial fibrosis and tubular atrophy is the main deleterious phenomenon involved. Stem cells are a promising therapeutic tool already validated in various ischemic diseases. Amniotic fluid-derived mesenchymal stem cells (af-MSCs), a subpopulation of multipotent cells identified in amniotic fluid, are known to secrete growth factors and anti-inflammatory cytokines. In addition, these cells are easy to collect, present higher proliferation and self-renewal rates compared with other adult stem cells (ASCs), and are suitable for banking. Consequently, af-MSCs represent a promising source of stem cells for regenerative therapies in humans. To determine the efficiency and the safety of af-MSC infusion in a preclinical porcine model of renal autotransplantation, we injected autologous af-MSCs in the renal artery 6 days after transplantation. The af-MSC injection improved glomerular and tubular functions, leading to full renal function recovery and abrogated fibrosis development at 3 months. The strong proof of concept generated by this translational porcine model is a first step toward evaluation of af-MSC-based therapies in human kidney transplantation.

show abstract

“…Using a similar method, we have subjected the rat full-term AF cells to immuno-selection against c-kit, anticipating the isolation of stem cells with a wider differentiation spectrum. C-kit, a cell surface receptor specific for stem cell factor (SCF), has been used to serve as a valuable marker to identify undifferentiated ESCs that have higher potential to expand and differentiate (Bai et al, 2012;Chen et al, 2009;Deberry et al, 1997). The c-kit sorting procedure has been shown to be able to enrich pluripotent stem cells using AFSC and ESC cultures (Bollini et al, 2011;Gekas et al, 2010).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Rat full term amniotic fluid harbors highly potent stem cells

Mun-Fun

Ferdaos

Hamzah

et al. 2015

Research in Veterinary Science

View full text Add to dashboard Cite

Human amniotic fluid-derived c-kit+ and c-kit− stem cells: growth characteristics and some differentiation potential capacities comparison

Cited by 21 publications

References 26 publications

Wnt Signaling Behaves as a “Master Regulator” in the Osteogenic and Adipogenic Commitment of Human Amniotic Fluid Mesenchymal Stem Cells

Wnt Signaling Behaves as a “Master Regulator” in the Osteogenic and Adipogenic Commitment of Human Amniotic Fluid Mesenchymal Stem Cells

Amniotic Fluid-Derived Mesenchymal Stem Cells Prevent Fibrosis and Preserve Renal Function in a Preclinical Porcine Model of Kidney Transplantation

Rat full term amniotic fluid harbors highly potent stem cells

Contact Info

Product

Resources

About