Human cleavage-stage embryo vitrification is comparable to slow-rate cryopreservation in cycles of assisted reproduction

Wilding, Martin; Capobianco, Clemente; Montanaro, N.; Kabili, Genc; Matteo, Loredana Di; Fusco, Enrico; Dale, Brian

doi:10.1007/s10815-010-9452-1

Cited by 33 publications

(18 citation statements)

References 24 publications

(37 reference statements)

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“…In the current study, we found that slow [9] and may have higher potential cell toxicity, which may be one of the major factors affecting development of embryos. Several studies have shown similar pregnancy and implantation rates between slow freezing and vitrification [13,27]. Vitrified embryo transfer is associated with lower hCG concentrations on day 12 post-embryo transfer compared with slow freezing embryo transfer [18].…”

Section: Discussionmentioning

confidence: 99%

“…The decision to switch the cryopreservation protocol was based on the reported high embryo survival rate, low risk of losing the chance of embryo transfer [10,11], and higher blastulation rates [12] by vitrification. However, some previous studies have found no difference in the pregnancy rate between vitrification and slow freezing [13,14]. Therefore, there is an obvious need to analyze the results of two cryopreservation protocols before totally abandoning slow freezing.…”

Section: Introductionmentioning

confidence: 99%

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Slow freezing should not be totally substituted by vitrification when applied to day 3 embryo cryopreservation: an analysis of 5613 frozen cycles

Zhu

Xue

Yang

et al. 2015

J Assist Reprod Genet

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Purpose This study aimed to compare slow freezing (SF) and vitrification (VT) techniques for day 3 embryo cryopreservation in infertile couples. Methods This retrospective cohort study enrolled 5613 infertile patients, with 7862 frozen-thawed day 3 embryos and 3845 vitrified-warmed day 3 embryos, from 2010 to 2014, at a single center. The rates of embryo survival, pregnancy, implantation, miscarriage, live birth, and live birth weight were compared between the two groups. Results A total of 5613 cycles with 5520 transfers were analyzed. Using SF, the rates of overall embryo survival and fully intact blastomeres were lower than those in VT (91.5 vs. 97.4 % and 68.7 vs. 92.3 %, respectively). The rate of good quality embryos after thawing/warming was lower in SF than in VT. In single frozen embryo transfer cycles (FETs), the pregnancy and implantation rates were similar between the two groups (35.0 vs. 40.8 % and 34.6 vs. 35.9 %, respectively). In double FETs, the pregnancy rate per cycle was also similar between the groups (58.8 vs. 58.4 %). The implantation rate per embryo transfer was significantly higher with SF than with VT (38.8 vs. 34.6 %). With adjustment for maternal age and the number of good quality embryos, differences in implantation rate remained significant (adjusted P value, SF vs. VT P<0.05). No independent effect was found for the method of cryopreservation on the pregnancy rate. No significant differences in the rates of miscarriage, live birth, and live birth weight were observed between the two techniques. Conclusions Despite the significantly low embryo survival rate, fully intact blastomere rate, and good quality embryo rate in SF, the pregnancy and implantation rates were not adversely affected in single and double FETs. SF yielded an equivalent miscarriage rate, live birth rate, and live birth weight compared with VT. The SF protocol to cryopreserve day 3 embryos still should be considered.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Slow freezing should not be totally substituted by vitrification when applied to day 3 embryo cryopreservation: an analysis of 5613 frozen cycles

Zhu

Xue

Yang

et al. 2015

J Assist Reprod Genet

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“…Technically, the method is able to decrease damaged embryo cells due to freezing. In addition, vitrification method is able to reduce damaged embryos due to freezing as critical temperature can be exceeded quickly (Wilding et al, 2010;Turathum et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of Insulin Transferrin Selenium Administration on Rat's Cultured In Vitro Embryo Post Warming After being Frozen using Vitrification Method

Widjiati¹,

Luqman²,

Hendrawan³

et al. 2018

Proceedings of the 1st International Conference in One Health (ICOH 2017)

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Success of embryo transfer is determined by availability of a plenty of amount of embryo stock. To make embryo stock remain in good quality, it is necessary to have an easy, cheap, simple and effective storage method. One of the storage methods is vitrification.Success of vitrification method is still disrupted due to decreased embryo quality post warming. Decreased embryo viability influences high implantation rate and gestation. Low implantation rate will result in low gestation rate. Therefore, study is needed to optimize vitrification medium so that it will be able to optimize cryoprotectant role to protect embryo due to temperature stressor in vitrification method.Administering Insulin Transferrin Selenium on vitrification medium is able to bind free radicals caused by temperature stressor due to frozen Insulin Transferrin Selenium which is a complex protein that is able to stimulate cell growth, prevent cell damage due to anti oxidant role in it so that it is able to maintain embryo viability post thowing. Insulin Transferrin Selenium is able to increase quality and viability of blastocyst resulted from in vitro culture.The study aims to prove Insulin Transferrin Selenium supplementation on vitrification medium is able to increase viability of rat embryo post warming. Steps of the research cover oocyte collection, embryo vitrification, warming, and in vitro culture. In vitro culture yields morula embryo with excellent quality and fits to be frozen using vitrification method.Insulin Transferin Selenium administration of 5,10 and 15 g/ml is able to increase embryo viability up to 100 %. Frozen embryo post warming and recultured for 5 hours , viability of morula embryo ofthe treatment group administered by Insulin Transferrin Selenium reached 73.4 % -83.4% whereas control group without being administered by InsulinTransferrin Selenium reached only 64.7 %. The research concludes that Insulin Transferrin Selenium.administration is able to increase viability of rat embryo at morula stage.

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“…In such cases, excess embryos are available for cryopreservation. Cryopreservation of surplus embryos increases the chances of conception, improves the cumulative pregnancy rates (AbdelHafez et al, 2010;Liebermann, 2009) and reduces cost of treatment (Wilding et al, 2010;Vajta and Nagy, 2006. Another benefit of cryopreservation is minimization of adverse effects of repeated ovarian stimulation (AbdelHafez et al, 2010;Shamonki and Oktay, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Among the different cryopreservation methods known, vitrification leads to higher survival rate of embryos (Wilding et al, 2010) because it prevents the formation of ice crystals inside and outside the cells (Rama Raju et al, 2005). The main disadvantage of this method is exposure of embryos to high concentrations of cryoprotectants in the vitrification solution, which may have a detrimental effect on them (Rezazadeh Valojerdi et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

H19 and MEST gene expression and histone modification in blastocysts cultured from vitrified and fresh two-cell mouse embryos

Jahangiri

Shahhoseini

Movaghar

2014

Reproductive BioMedicine Online

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Human cleavage-stage embryo vitrification is comparable to slow-rate cryopreservation in cycles of assisted reproduction

Abstract: These data suggest that vitrification of human embryos during assisted reproduction cycles achieves comparable success rates to fresh cycles and therefore can be applied in the laboratory of assisted reproduction.

Cited by 33 publications

References 24 publications

Slow freezing should not be totally substituted by vitrification when applied to day 3 embryo cryopreservation: an analysis of 5613 frozen cycles

Slow freezing should not be totally substituted by vitrification when applied to day 3 embryo cryopreservation: an analysis of 5613 frozen cycles

Effect of Insulin Transferrin Selenium Administration on Rat's Cultured In Vitro Embryo Post Warming After being Frozen using Vitrification Method

H19 and MEST gene expression and histone modification in blastocysts cultured from vitrified and fresh two-cell mouse embryos

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