Human-like collagen promotes the healing of acetic acid-induced gastric ulcers in rats by regulating NOS and growth factors

Xing, Mimi; Fu, Rongzhan; Liu, Yannan; Wang, Pan; Ma, Ping; Zhu, Chenhui; Fan, Daidi

doi:10.1039/d0fo00288g

Cited by 22 publications

(10 citation statements)

References 48 publications

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“…matsutake peptides on gastric injury might be induced by promoting the expression of certain growth factors and inducing tissue reconstruction. In addition, angiogenesis is essential for wound healing, and VEGF accelerates gastric mucosal recovery by stimulating the rebuilding of the mucosal microvascular network and facilitating the delivery of oxygen and nutrients to the site of injury. , Our research confirmed a decrease in VEGF protein expression in gastric tissues after ethanol stimulation, which is consistent with previous research . Our results also suggested that supplementation with SDLKHFPF and SDIKHFPF could upregulate the expression of VEGF protein.…”

Section: Discussionsupporting

confidence: 91%

“…29,30 Our research confirmed a decrease in VEGF protein expression in gastric tissues after ethanol stimulation, which is consistent with previous research. 31 Our results also suggested that supplementation with SDLKHFPF and SDIKHFPF could upregulate the expression of VEGF protein. Thus, we deduced that T. matsutake peptides might ameliorate ethanol-induced gastric injury by promoting the expression of angiogenic factors.…”

Section: ■ Discussionsupporting

confidence: 59%

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Tricholoma matsutake-Derived Peptides Show Gastroprotective Effects against Ethanol-Induced Acute Gastric Injury

et al. 2021

J. Agric. Food Chem.

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Acute gastric injury caused by ethanol is a frequent disorder of the gastrointestinal tract. In this study, we investigated the potential gastroprotective effects of Tricholoma matsutake-derived peptides against ethanol-triggered acute gastric injury and the associated mechanisms. Peptides SDLKHFPF and SDIKHFPF significantly attenuated the ethanol-induced decrease in GES-1 cell survival (82.39 ± 1.93 and 80.10 ± 1.08% vs 56.58 ± 1.86%), inhibited GES-1 cell apoptosis, and alleviated the ethanol-induced gastric mucosal injury (64.76 ± 3.98 and 49.29 ± 3.25%), ulcer index (3.33 ± 0.47 and 4.67 ± 0.47 vs 6.67 ± 0.47), and histopathological changes in mice. Peptide treatment inhibited the phosphorylation and nuclear translocation of nuclear factor kappa B (NF-κB), the secretion of tumor necrosis factor-α, interleukin-6, and endothelin-1. In addition, T. matsutake peptide pretreatment increased growth factor secretion, upregulated B-cell lymphoma-2, downregulated Bcl-2-associated X (Bax), and cleaved cysteinyl aspartate specific proteinase 3, thereby promoting gastric cell survival. These findings strongly suggest that T. matsutake peptides attenuate ethanol-induced inflammatory responses and apoptosis by suppressing NF-κB signaling activation, thereby enhancing gastric epithelial barrier functions.

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Section: Discussionsupporting

confidence: 91%

Section: ■ Discussionsupporting

confidence: 59%

Tricholoma matsutake-Derived Peptides Show Gastroprotective Effects against Ethanol-Induced Acute Gastric Injury

et al. 2021

J. Agric. Food Chem.

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“…The specimens were fixed in 10% neutral formalin then prepared for paraffin blocks. The blocks were cut into 5-mm sections and stained with hematoxylin and eosin for examining the histological structure 19 , periodic acid-Schiff (PAS) for evaluating mucosal glycoprotein production variations in the mucosa of the stomach 20 , and Masson’s Trichrome for detecting collagen fiber deposition 21 .…”

Section: Methodsmentioning

confidence: 99%

Curcumin ameliorated low dose-Bisphenol A induced gastric toxicity in adult albino rats

Ismail

El-Meligy

2022

Sci Rep

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Bisphenol A (BPA) is one of the most common worldwide chemicals involved in the industry of polycarbonate plastics, medical devices, and pharmaceuticals. Forty three-month-old albino rats were randomly classified into four groups. Group Ӏ received a daily corn oil dose (5 mL/kg/ body weight, BW) through a gastric tube for one month, Group ӀӀ received a daily dose of Curcumin (200 mg/kg body weight (B.W.) through a gastric tube for one month, Group ӀӀӀ received a daily dose of BPA (0.5 μg/kg B.W.) through a gastric tube for one month and Group ӀV received concomitant daily doses of Bisphenol A and Curcumin as the regimen described in groups ӀӀ and ӀӀӀ. The rats were sacrificed, and glandular portion of stomach was dissected and processed for light, immunohistochemical and ultrastructural study. BPA induced destructed gastric glands, dilated congested blood vessels, submucosal oedema, decreased PAS-positive reactivity, increased collagen fibres deposition, decrease in the positive BCL2 immunoexpression, increased positive PCNA immunoexpression, reduction in the gastric mucosal height and destructive changes in the enteroendocrine, chief and parietal cells. Curcumin coadministration provoked an obvious improvement in the gastric structure. BPA exposure has toxic effects on the glandular portion of the stomach in rats. Otherwise, Curcumin coadministration has exhibited protective impact on the architecture of the stomach.

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“…Type I collagen (Col I), which promotes cellular proliferation, migration, and differentiation, is the support structure of the skin. [ 37 ] Figure 9 a,b shows that among the diabetic chronic wounds in the different groups, the wounds in the ES therapy‐PHTB(TA‐siRNA) hydrogel groups have the highest levels of Col I, which indicates that the combination therapy promotes collagen production. Significant amounts of Col I are detected in fibroblasts, which were labeled with vimentin.…”

Section: Resultsmentioning

confidence: 99%

A Combination Therapy Using Electrical Stimulation and Adaptive, Conductive Hydrogels Loaded with Self‐Assembled Nanogels Incorporating Short Interfering RNA Promotes the Repair of Diabetic Chronic Wounds

Lei

Fan

2022

Advanced Science

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In addition to oxidative stress and impaired angiogenesis, the overexpression of metalloproteinases (MMPs) and proinflammatory cytokines, which are promoted by hyperglycemia, causes chronic inflammation in diabetic wounds. Herein, TA-siRNA nanogels are prepared for the first time on the basis of the self-assembling interaction between tannic acid (TA) and short interfering RNA (siRNA). The efficient, biodegradable nanogels are cross-linked with poly(vinyl alcohol) (PVA), human-like collagen (HLC), TA, and borax to prepare adaptive, conductive PHTB (TA-siRNA) hydrogels. In response to high levels of reactive oxygen species (ROS), the ROS-responsive borate ester bonds in the hydrogels are oxidized and broken, and TA-siRNA nanogels are released into cells to reduce the expression of the MMP-9. Moreover, the TA and HLC promote collagen expression, reduce inflammation, and ROS level. It is found that electrical stimulation (ES) promotes the in vivo release of TA-siRNA nanogels from PHTB (TA-siRNA) hydrogels and endocytosis of the nanogels. The combination therapy using ES and PHTB (TA-siRNA) hydrogels accelerates the healing of diabetic wounds by reducing the levels of ROS and MMP-9 and promoting the polarization of macrophages, production of collagen, and angiogenesis. This study provides insights on the design of functional gene-delivery and efficient therapeutic strategies to promote the repair of diabetic chronic wounds.

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Human-like collagen promotes the healing of acetic acid-induced gastric ulcers in rats by regulating NOS and growth factors

Abstract: Human-like collagen promotes the healing of acetic acid-induced gastric ulcers in rats: possible mechanisms, promotes the expression of growth factors, regulates NOS activity, promotes collagen deposition, and reduces inflammatory factors.

Cited by 22 publications

References 48 publications

Tricholoma matsutake-Derived Peptides Show Gastroprotective Effects against Ethanol-Induced Acute Gastric Injury

Tricholoma matsutake-Derived Peptides Show Gastroprotective Effects against Ethanol-Induced Acute Gastric Injury

Curcumin ameliorated low dose-Bisphenol A induced gastric toxicity in adult albino rats

A Combination Therapy Using Electrical Stimulation and Adaptive, Conductive Hydrogels Loaded with Self‐Assembled Nanogels Incorporating Short Interfering RNA Promotes the Repair of Diabetic Chronic Wounds

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