Human Lymphocyte Sub-Populations and K Cells

Abstract

Peripheral blood lymphocytes from 19 normal subjects were examined for surface Ig (SIg) and capacity to form rosettes with normal and neuraminidase-treated sheep erythrocytes and with chicken erythrocytes sensitised with IgG antibody. Information on the relationship between the presence of SIg and capacity to form rosettes was obtained by combined tests and depletion experiments. By these means, a population of lymphocytes with Fc receptors, but lacking SIg (mean 14.6%) was defined and shown to correlate closely with cytotoxic activity for antibody-sensitised target cells. Indirect evidence was also obtained that these lymphocytes, which are regarded as the major population of antibody-dependent cytotoxic cells, are capable of forming rosettes with normal and neuraminidase-treated sheep erythrocytes. The nature of these cells is briefly discussed.

Demonstration of Fc Receptors on the Surface of B Lymphocytes

“…This is in agreement with the findings of Brain and Marston [1] and with our previous results [20]. By con trast, Fr0land et al [7], Samarut et al [18], Gergely et al [8] and Van Oers et al [21] have concluded, from tests on suspensions of PBL which had been depleted by various procedures, and from double-marker tests, that most lymphocytes with SIg are largely a separate population from those with Fc re ceptors.…”

“…In partic ular, the relationship of B lymphocytes, identified by readily demonstrable surface immunoglobulin (SIg), to Fc-receptor cells, is controversial. Some workers have con cluded that most Fc-receptor cells are B cells [3], others consider that the two are largely distinct subpopulations [7,8,21], while a third view is that Fc-receptor cells include most of the B cells and also a popu lation of non-B cells [1,20], In this paper we provide evidence in support of this last view and report findings which appear to explain, at least in part, the discrepant con clusions.…”

Decreased Antibody-Dependent Cellular Cytotoxicity in Preleukemic Syndromes

“…A number of effector cells includ ing subpopulations of lymphocytes [Nel son et al, 1976;Sandilans et al, 1976;Evans et al, 1978], monocyte-macrophage cells [Mantovani et al, 1977] and neutro phils [Hafeman and Lucas, 1979] have been shown to mediate ADCC. Recently, several workers described the decreased ADCC potential of the peri pheral blood from patients with different types of leukemia [Faldt and Ankerst, 1979;, Fernandes et al, 1979;Platsoucas et al, 1980;Jakobisiak et al, 1981a], cancer [Stratton et al, 1977] and primary immu nodeficiency disorders [Karen et al, 1978].…”

In vivo Modulation of Human Lymphocyte Fcγ-Receptors in Response to Oral Antigen (Cows’ Milk) Challenge

“…Human B lymphocytes possess Fcy-receptors of relatively low avidity for EA as compared with these non-B lymphocytes with Fcy-receptors [2,10,13]. This latter group with high avidity Fcy-receptors, containing the effector cells in antibody-dependent cell cytotoxicity (K cells) [12] and a proportion of T (suppressor?) cells (Ty-cells) [8], are therefore more likely to bind immune complexes.…”