Human neutrophil alloantigen‐1a, ‐1b, ‐2, ‐3a and ‐4a frequencies in Brazilians

Norcia, Angela Maria Matico Ikeda; Sugano, E. Y. K.; Chiba, Akemi Kuroda; Moritz, Elyse; Guirao, Fabio P.; Yamamoto, Masahiro; Bordin, José Orlando

doi:10.1111/j.1399-0039.2009.01357.x

Cited by 11 publications

(24 citation statements)

References 22 publications

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“…They can lead to transfusion‐related acute lung injury (TRALI), febrile transfusion reactions, refractoriness to granulocyte transfusion, and neutropenia after stem cell transplantation (7). HNA alloantibodies can also be responsible for neonatal neutropenia (7, 8). The probability for developing immune response depends among other factors on the existing antigens of the individual.…”

Section: Introductionmentioning

confidence: 99%

Human neutrophil alloantigen genotype frequencies among blood donors with Turkish and German descent

et al. 2011

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Antibodies against the human neutrophil antigens (HNA) are able to stimulate transfusion reactions, autoimmune and neonatal neutropenia. The aim of this study was to determine the HNA allele frequencies in the largest ethnic minority group in Germany in comparison with the German population for predicting the risk of alloimmunization and associated transfusion reactions, as well as the risk of developing neonatal neutropenia for the newborn of racial mixed couples. However, there exists no data about HNA genotype distribution in Turkish population. DNA was isolated from blood samples of 119 German and 118 Turkish blood donors and typed them for HNA-1, -3, -4, and -5 by using a commercial polymerase chain reaction kit with sequence-specific primers (SSP-PCR) and compared the HNA genotype distribution of both groups. In German blood donors, the gene frequencies for HNA-1a and HNA-1b were 0.391 and 0.601, for HNA-3a and -3b, 0.744 and 0.256, for HNA-4a and -4b, 0.908 and 0.092, and for HNA-5a and -5bw, 0.731 and 0.269. In Turkish blood donors, we observed 0.420/0.564, 0.737/0.263, 0.881/0.119, and 0.754/0.246 for HNA-1a/1b, -3a/3b, -4a/4b, and -5a/5bw. No statistic significant difference between genotypes in these populations was observed. This study is the first to report HNA gene frequencies in a Turkish population. It showed that there is no difference of HNA genotype in blood donors with Turkish descent in comparison with German blood donors. The alternating transfusion of blood and blood components is no increased risk for developing alloantibodies against HNA antigens. In pregnancy of mixed couples no special screening programs for HNA are necessary.

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Section: Introductionmentioning

confidence: 99%

Human neutrophil alloantigen genotype frequencies among blood donors with Turkish and German descent

et al. 2011

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“…The SLC44A2*2 allele frequencies vary among populations and range in the Han population from 38.2% (Nanning) to 26.2% (Guangzhou) . Although TRALI is much less frequently reported in China than in the United States, the susceptible allele SLC44A2*2 is more prevalent in any East Asian population compared to 17% in Caucasians and 7% in African Americans …”

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confidence: 99%

“…HNA-3a (SLC44A2*1 allele) and HNA-3b (SLC44A2*2 allele) are defined by a single-nucleotide polymorphism (SNP) at Position 461G>A (rs2288904). 7,8 The SLC44A2*2 allele frequencies vary among populations [9][10][11][12][13][14][15][16][17][18][19][20][21] and range in the Han population from 38.2% (Nanning) 18 to 26.2% (Guangzhou). 22 Although TRALI is much less frequently reported in China 22,23 than in the United States, 24 the susceptible allele SLC44A2*2 is more prevalent in any East Asian population compared to 17% in Caucasians 9 and 7% in African Americans.…”

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Genotype frequency of human neutrophil antigen‐3 polymorphisms in the Yi, Han, and Tibetan populations of China

et al. 2015

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Background Human neutrophil antigen-3 (HNA-3) alloantibodies can cause fatal transfusion-related acute lung injury (TRALI). Most frequencies of SLC44A2 alleles encoding the HNA-3a/b antigens have been established in Han individuals by PCR-SSP. We sequenced a SLC44A2 gene fragment and determined its allele frequencies in 3 ethnicities of China. Study design and methods Genomic DNA was extracted from 448 samples of 100 blood donors of Yi ethnicity in Xichang, Liangshan; 248 Han in Nanjing, Jiangsu; and 100 Tibetan in Lhasa, Tibet. A PCR-SSP was applied to determine the phase of 2 SNPs; SLC44A2 haplotypes were constructed. Results In the 567 nucleotides of the SLC44A2 gene covered by our sequencing approach in Han individuals, we detected the known 331-44G>A (rs12972963) and 461G>A (rs2288904) polymorphisms. In the 243 nucleotides sequenced in Yi and Tibetan populations, we detected the known 461G>A and 503-15T>C (rs1560711) polymorphisms. A PCR-SSP for the common HNA-3a/b SNP was 100% concordant. The frequencies of the HNA-3a allele were 0.58, 0.66 and 0.69 in Yi, Han (Nanjing) and Tibetan, respectively (0.42, 0.34 and 0.31 for HNA-3b). Conclusions The Yi population of China had the highest frequency of blood donors at risk of harboring anti-HNA-3a compared to any population studied so far. We confirmed that the underlying SLC44A2*2 allele is more common in China than in any European or African populations.

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“…The frequency of the SNP indicative of the SLC44A2*1 and SLC44A2*2 alleles is known for Caucasian, African American, Han Chinese, Japanese, Tunisian, Brazilian, Thai, and other populations . Chu and colleagues applied an algorithmic approach to predict HNA antigens from whole genome sequencing (WGS) data.…”

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confidence: 99%

“…10,15,16 The Leu153Phe (457C>T; rs147820753) has been reported to yield false-negative genotyping results for HNA-3a if the specific primer encompasses this variation. 17 The frequency of the SNP indicative of the SLC44A2*1 and SLC44A2*2 alleles is known for Caucasian, 18,19 African American, 18 Han Chinese, 20 Japanese, 21 Tunisian, 22 Brazilian, 23 Thai, 24,25 and other populations. [26][27][28][29][30] Chu and colleagues 31 applied an algorithmic approach to predict HNA antigens from whole genome sequencing (WGS) data.…”

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confidence: 99%

Full‐length nucleotide sequences of 30 common SLC44A2 alleles encoding human neutrophil antigen‐3

et al. 2015

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Background HNA-3a alloantibodies can cause severe transfusion-related acute lung injury (TRALI). The frequency of the single nucleotide polymorphisms (SNPs) indicative of the two clinically relevant HNA-3a/b antigens are known in many populations. In the present study, we determined the full length nucleotide sequence of common SLC44A2 alleles encoding the choline transporter-like protein-2 (CTL2) that harbors HNA-3a/b antigens. Study design and methods A method was devised to determine the full length coding sequence and adjacent intron sequences from genomic DNA by 8 polymerase chain reaction (PCR) amplifications covering all 22 SLC44A2 exons. Samples from 200 African American, 96 Caucasian, 2 Hispanic and 4 Asian blood donors were analyzed. We developed a decision tree to determine alleles (confirmed haplotypes) from the genotype data. Results A total of 10 SNPs were detected in the SLC44A2 coding sequence. The non-coding sequences harbored an additional 28 SNPs (1 in the 5’-untranslated region (UTR); 23 in the introns; and 4 in the 3’-UTR). No SNP indicative of a non-functional allele was detected. The nucleotide sequences for 30 SLC44A2 alleles (haplotypes) were confirmed. There may be 66 haplotypes among the 604 chromosomes screened. Conclusions We found 38 SNPs, including 1 novel SNP, in 8192 nucleotides covering the coding sequence of the SLC44A2 gene among 302 blood donors. Population frequencies of these SNPs were established for African Americans and Caucasians. Because alleles encoding HNA-3b are more common than non-functional SLC44A2 alleles, we confirmed our previous postulate that African American donors are less likely to form HNA-3a antibodies compared to Caucasians.

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Human neutrophil alloantigen‐1a, ‐1b, ‐2, ‐3a and ‐4a frequencies in Brazilians

Cited by 11 publications

References 22 publications

Human neutrophil alloantigen genotype frequencies among blood donors with Turkish and German descent

Human neutrophil alloantigen genotype frequencies among blood donors with Turkish and German descent

Genotype frequency of human neutrophil antigen‐3 polymorphisms in the Yi, Han, and Tibetan populations of China

Full‐length nucleotide sequences of 30 common SLC44A2 alleles encoding human neutrophil antigen‐3

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