Human Oocyte Abnormalities: Basic Analyses and Clinical Applications

Rawe, V.Y.; Combelles, Catherine M.H.

doi:10.1007/978-1-60327-392-3_13

Cited by 4 publications

(8 citation statements)

References 182 publications

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“…In mammals, there is less species alteration in ZP3composition; this may illustrate why penetration of the zona pellucida by spermatozoa of nearly related mammalian species is sometimes feasible, whilst it is rare amongst lower beasts ( Carlson, 2018 ). The normal maturation of follicles and oocytes rely on presence of the ZP ( Rawe & Combelles, 2009 ). The zona pellucida plays an essential role in the sperm-oocyte interaction, acrosomal reaction, sufficient block to polyspermy, preimplantation embryos and following troubles in developmental progression ( Ebner et al ., 2008 ).…”

Section: Oocyte Characteristics In Agingmentioning

confidence: 99%

“…PVS is one of the most significant parts of extracytoplasmic maturation. There are three kinds of PVS classifications that are possible: immediately opposed to the plasma membrane, apart and recognizable, exaggerated ( Rawe & Combelles, 2009 ; Talbot & Dandekar, 2003 ). Despite these differences, there is a clear disagreement that still remains over PVS arrangement regarding what may be typical or deviant from healthy.…”

Section: Oocyte Characteristics In Agingmentioning

confidence: 99%

“…Because of the lack of knowledge, we can understand that a large PVS may break the connection between the cumulus cells and the oocyte, specifically by means of transzonal projections and gap junctions. On the other hand, it may be useful to disconnect some ways of cell communication at the suitable time, and there may be a mechanism by which PVS controls oocyte competence ( Rawe & Combelles, 2009 ). Overall, morphological assessments of the three extracellular parts of the oocyte yields a definite measure of oocyte evaluation while not freely rendering predictors of oocyte quality.…”

Section: Oocyte Characteristics In Agingmentioning

confidence: 99%

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Oocyte quality and aging

Moghadam¹,

Moghadam²,

Hemadi³

et al. 2021

JBRA

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It is well known that female reproduction ability decreases during the forth decade of life due to age-related changes in oocyte quality and quantity; although the number of women trying to conceive has today increased remarkably between the ages of 36 to 44. The causes of reproductive aging and physiological aspects of this phenomenon are still elusive. With increase in the women's age, during Assisted Reproductive Technologies (ART) we have perceived a significant decline in the number and quality of retrieved oocytes, as well as in ovarian follicle reserves. This is because of increased aneuploidy due to factors such as spindle apparatus disruption; oxidative stress and mitochondrial damage. The aim of this review paper is to study data on the potential role of the aging process impacting oocyte quality and female reproductive ability. We present the current evidence that show the decreased oocyte quality with age, related to reductions in female reproductive outcome. The aging process is complicated and it is caused by many factors that control cellular and organism life span. Although the factors responsible for reduced oocyte quality remain unknown, the present review focuses on the potential role of ovarian follicle environment, oocyte structure and its organelles. To find a way to optimize oocyte quality and ameliorate clinical outcomes for women with aging-related causes of infertility.

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Section: Oocyte Characteristics In Agingmentioning

confidence: 99%

Section: Oocyte Characteristics In Agingmentioning

confidence: 99%

Section: Oocyte Characteristics In Agingmentioning

confidence: 99%

See 1 more Smart Citation

Oocyte quality and aging

Moghadam¹,

Moghadam²,

Hemadi³

et al. 2021

JBRA

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“…There is considerable interest in identifying biomarkers of developmental competency. While biomarkers within the oocyte are an obvious choice, testing for developmental competency of oocytes in a non-invasive manner, without compromising its chances for fertilisation, embryo development and implantation, is an area of ongoing research (Sinclair et al, 2008;Rawe & Combelles, 2009). Therefore, development of competency biomarkers for materials that are routinely discarded during ART prior to fertilisation serve as attractive alternative to the oocyte.…”

Section: Ovulation and Developmental Competencymentioning

confidence: 99%

“…Due to the bidirectional communication that occurs throughout development between the oocyte and CC, and their juxtaposed positioning, the CCs are included in the group of materials discarded during ART procedures and have been utilised to investigate for biomarkers of oocyte developmental competency. There are clear benefits of profiling maternally-derived mRNA and proteins as they are key during early embryonic development and their expression levels within the COC, making them ideal biomarkers with potential prognostic value in terms of oocyte quality (Rawe & Combelles, 2009).…”

Section: Ovulation and Developmental Competencymentioning

confidence: 99%

Effects of BMP15 and GDF9 on Ovine Oocytes in an In Vitro Maturation System

Singh¹

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<p>Oocyte developmental competency is the intrinsic measure of oocyte quality and the capacity for a mature oocyte to support the early stages of embryo development and implantation. Oocyte-secreted factors (OSFs), such as growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15), play a pivotal role in regulating the synchrony of various complex maturation events within the cumulus-oocyte complex (COC) through the induction of paracrine and endocrine signalling. These proteins act synergistically to influence the proliferation and differentiation of granulosa cells (GCs), cumulus cell (CC) expansion, promote survival, ovulation, the attainment of developmental competency and fertility. Species-specific ratios suggest that poly-ovulatory mammals have increased fecundity due to high ratios of GDF9:BMP15, which is directly reflected in their large litter size. Interestingly, it has also been found that higher ratios of GDF9:BMP15 also increased blastocyst rate in sheep implying that these embryos develop from oocytes that are more developmentally competent. In this study, I investigated the hypothesis that supplementing a commercial in vitro maturation (IVM) system with a high ratio of GDF9:BMP15 would increase the developmental competency sheep oocytes; a species with low-moderate litter size. To test this hypothesis, ovine oocytes were matured in a biphasic IVM system containing GDF9 and BMP15 at three divergent ratios (1:6, 1:1, 6:1). The results herein show that the 6:1 ratio resulted in higher levels of reagent transfer to the ovine oocyte through gap junctions (GJs) after 24 hours of incubation. Similarly, it was also observed that at the higher ratio, glutathione (GSH) levels were higher at 7.5 hours of incubation. The high GDF9:BMP15 ratio also facilitated the increased consumption of pyruvate by the COC consistently throughout the culture period. Importantly, the high GDF9:BMP15 ratio showed higher expression of the gene that encodes GJ (CX43) at 24 hours relative to the control. It was also demonstrated through decreased apoptotic factor (BAX:BCL2) ratios, that the addition of OSFs, regardless of ratio, protected against cell death. In summary, this study provides novel results that support the notion that a high GDF9:BMP15 ratio improves oocyte quality by delaying the timing of meiotic resumption. This subsequently improves the transport of key metabolites and antioxidants to protect against oxidative stress and cell death and aid in the completion of maturation, ultimately resulting in the increased developmental competency observed in high fecundity poly-ovulatory species.</p>

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Determinants of Oocyte Quality: Impact on In Vitro Fertilization Failures

Combelles

Rawe

2012

Oogenesis

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Human Oocyte Abnormalities: Basic Analyses and Clinical Applications

Cited by 4 publications

References 182 publications

Oocyte quality and aging

Oocyte quality and aging

Effects of BMP15 and GDF9 on Ovine Oocytes in an In Vitro Maturation System

Determinants of Oocyte Quality: Impact on In Vitro Fertilization Failures

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