Human papillomavirus DNA, HPV L1 capsid protein and p16INK4a protein as markers to predict cervical lesion progression

Hu, Huiyan; Zhao, Jingjing; Wen, Yu; Zhao, Junwei; Wang, Zhewei; Lin, Jia Wei; Yu, Yunyun; Han, Lu; Wang, Lu; Zhu, Honghao; Li, Fang

doi:10.1007/s00404-018-4931-1

Cited by 18 publications

(13 citation statements)

References 28 publications

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“…Furthermore, we measured expression levels of P16, which has been reported to participate in cell cycle regulation and is now used for the clinical detection of HSIL in cervical cancer patients. [30][31][32][33][34] We found that LRP11 expression correlated with cancer differentiation and could be used as a prognostic marker, an advantage over P16 measurement. Consistent with our results, Hoang's study also found that osteosarcoma patients with positive LRP5 expression showed a trend on decreased of event-free survival.…”

Section: Discussionmentioning

confidence: 82%

<p>The role of low density lipoprotein receptor-related protein 11 as a tumor promoter in cervical cancer</p>

Wang

Han

You

et al. 2019

CMAR

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Background It is unclear whether low density lipoprotein receptor-related protein 11 (LRP11), a newly found lipoprotein receptor regulatory protein, has the carcinogenic effects in cervical cancer. Methods Bioinformatics analysis, immunohistochemical (IHC) staining and evaluation, cell proliferation assay, flow cytometry, transwell migration and invasion assays, Western blotting, growth of LRP11-silenced cells in athymic nude mice were performed in this research. Results We found that LRP11 expression was higher in high-grade squamous intraepithelial lesions (HSIL) and cervical cancer tissue than in normal cervix, and high expression of LRP11 was associated with differentiation degree ( P =0.0266), indicating poor prognosis ( P =0.0210). The silencing of LRP11 in SiHa and CaSki cell lines inhibited cell proliferation, reduced migration and invasion and suppressed cell growth in nude mice, which possibly related to cell cycle protein regulation of CDK 2/4, cyclin D1/E1, MMP-2/9, and VEGF. Furthermore, LRP11 showed substantial positive correlation with P16 in vivo and in vitro. Conclusion LRP11 plays important roles in proliferation, migration and invasion, with the potential to be a useful prognostic marker and therapeutic target for patients with HSIL and cervical cancer.

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Section: Discussionmentioning

confidence: 82%

<p>The role of low density lipoprotein receptor-related protein 11 as a tumor promoter in cervical cancer</p>

Wang

Han

You

et al. 2019

CMAR

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“…Similar findings have been reported by Lopez‐Beltran et al 25 who analyzed HPV in 76 cases of UC and found a positivity rate of 32.8% using the IHC for the HPV capsid antigen using a polyclonal antibody, whereas only 15.7% of the cases were positive for HPV 16/18‐DNA using non‐isotopic in situ hybridization. These findings are more likely due to the nonspecific binding of the IHC HPV capsid antibody and its lower sensitivity in advanced HPV‐related neoplasm with integrated viral DNA 14,26 . In fact, a number of HPV IHC tests with antibodies against either E6 or E7 oncoproteins or HPV L1 capsid protein have not yet been validated for clinical use in HPV detection.…”

Section: Discussionmentioning

confidence: 99%

“…These findings are more likely due to the nonspecific binding of the IHC HPV capsid antibody and its lower sensitivity in advanced HPV‐related neoplasm with integrated viral DNA. 14 , 26 In fact, a number of HPV IHC tests with antibodies against either E6 or E7 oncoproteins or HPV L1 capsid protein have not yet been validated for clinical use in HPV detection.…”

Section: Discussionmentioning

confidence: 99%

Detection of HPV infection in urothelial carcinoma using RNAscope: Clinicopathological characterization

et al. 2021

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Background Human papillomavirus (HPV) is a well‐established mucosotropic carcinogen, but its impact on urothelial neoplasm is unclear. We aimed to clarify the clinical and pathological features of HPV‐related urothelial carcinoma (UC). Methods Tissue samples of 228 cases of UC were obtained from the bladder, upper and lower urinary tract, and metastatic sites to construct a tissue microarray. The samples were analyzed for the presence of HPV by a highly sensitive and specific mRNA in situ hybridization (RISH) technique (RNAscope) with a probe that can detect 18 varieties of high‐risk HPV. We also conducted immunohistochemistry (IHC) for a major HPV capsid antibody and DNA‐PCR. Results The HPV detection rates varied among the methods; probably due to low HPV copy numbers in UC tissues and the insufficient specificity and sensitivity of the IHC and PCR assays. The RISH method had the highest accuracy and identified HPV infection in 12 (5.2%) of the cases. The histopathological analysis of the HPV‐positive UC showed six cases of usual type UC, five cases of UC with squamous differentiation (UC_SqD), and one case of micropapillary UC. The HPV detection rate was six‐fold higher in the cases of UC_SqD than in the other variants of UC (odds ratio [OR] =8.9, p = 0.002). In addition, HPV infection showed a significant association with tumor grade (OR =9.8, p = 0.03) and stage (OR =4.7, p = 0.03) of UC. Moreover, the metastatic rate was higher in HPV‐positive than in negative UC (OR =3.4). Conclusion These data indicate that although the incidence of HPV infection in UC is low, it is significantly associated with squamous differentiation and poor prognosis. Furthermore, our observations show that RNAscope is an ideal method for HPV detection in UC compared with the other standard approaches such as IHC and PCR assays.

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“…reported that the HPV16 L1 vaccine induced not only B‐cell responses but also HPV16 L1‐specific T‐cell responses. In addition, HPV infections in cutaneous warts differ from those in cervical lesions in that the HPV type‐specific L1 protein, the target protein for the vaccines, is regularly synthesized in the upper epithelial layers, whereas it is often downregulated in cervical lesions . Furthermore, cross‐protection between HPV types may be involved.…”

Section: Characteristics Of the Study Patientsmentioning

confidence: 99%

Three cases of recalcitrant cutaneous warts treated with quadrivalent human papillomavirus (HPV) vaccine: the HPV type may not determine the outcome

Hayashi¹,

Matsumoto²,

Mitsuishi

2019

Br J Dermatol

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Human papillomavirus DNA, HPV L1 capsid protein and p16INK4a protein as markers to predict cervical lesion progression

Cited by 18 publications

References 28 publications

<p>The role of low density lipoprotein receptor-related protein 11 as a tumor promoter in cervical cancer</p>

<p>The role of low density lipoprotein receptor-related protein 11 as a tumor promoter in cervical cancer</p>

Detection of HPV infection in urothelial carcinoma using RNAscope: Clinicopathological characterization

Three cases of recalcitrant cutaneous warts treated with quadrivalent human papillomavirus (HPV) vaccine: the HPV type may not determine the outcome

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