Human papillomavirus type‐16‐related DNA in genital Bowen's disease and in bowenoid papulosis

Abstract

32P-labelled DNA of HPV 16 which has been isolated and molecularly cloned from a cervical carcinoma (Dürst et al., 1983) was used to screen the cellular DNAs obtained from 20 different biopsies of Morbus Bowen or Bowenoid papulosis, respectively, by Southern blot analysis. Under conditions of differing stringency for the hybridization, HPV 16 DNA or related sequences were identified in 6 out of 10 cases of Morbus Bowen (4 out of 5 from a genital localization) and in 8 out of 10 biopsies from Bowenoid papulosis. One additional case of the latter disease contained DNA sequences of an HPV type not yet classified. There is evidence for the presence of another HPV DNA in two of the HPV-16-positive tumors. A large number of normal genital tissue samples were negative for HPV DNA.

Papillomavirus genomes in human cervical tumors: analysis of their transcriptional activity.

“…HPV-16 DNA was detected in a small number of condylomata acuminata (8) and Buschke-Lowenstein tumors (unpublished data), in 80% of bowenoid lesions (9), and in atypical flat condylomata of the cervix displaying abnormal mitotic figures (6). The latter are regarded as possible precursers of cervical carcinomas (6,10,11).…”

The Expression of Human Papillomavirus Type 18 E6 Protein in Bacteria and the Production of Anti-E6 Antibodies

“…These include HPV-6, HPV-11, HPV-16 and HPV-18 which have been associated with tumours of the genital tract. HPV-6 and HPV-11 DNAs have been identified in benign lesions whereas HPV-16 and HPV-18 DNAs have been found in malignant lesions (Gissmann et al, 1983;Ikenberg et al, 1983;Boshart et al, 1984;Crum et al, 1984). DNAs are also present in a number of cell lines derived from human cervical carcinomas, and in addition these DNAs are transcribed (Schwarz et al, 1985 ;Yee et al, 1985).…”

BritishAssociation ofDermatologists' guidelines for the management of squamous cell carcinomain situ(Bowen's disease) 2014

“…117,[125][126][127][128][129][130][131][132][133][134][135][136][137][138] HPV-associated digital SCC suggests the possibility of genital digital spread as a mechanism of tumour induction, 115 and patients and partners should be followed up for digital and genital HPV-associated lesions. 138 Management of periungual SCC is usually local excision, Mohs micrographic surgery or distal phalanx amputation.…”