2008
DOI: 10.1002/prca.200780048
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Human pathogenic streptococcal proteomics and vaccine development

Abstract: Gram-positive streptococci are non-motile, chain-forming bacteria commonly found in the normal oral and bowel flora of warm-blooded animals. Over the past decade, a proteomic approach combining 2-DE and MS has been used to systematically map the cellular, surface-associated and secreted proteins of human pathogenic streptococcal species. The public availability of complete streptococcal genomic sequences and the amalgamation of proteomic, genomic and bioinformatic technologies have recently facilitated the ide… Show more

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Cited by 40 publications
(44 citation statements)
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“…Although several surface proteins and virulence factors have been previously assessed as vaccine candidates [34], there is no commercial vaccine available to prevent GAS infection. Following a proteomic analysis of an M1T1 cell wall extract, we selected three anchorless proteins; ADI, FBA and TF; for further characterization as putative GAS vaccine candidates.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Although several surface proteins and virulence factors have been previously assessed as vaccine candidates [34], there is no commercial vaccine available to prevent GAS infection. Following a proteomic analysis of an M1T1 cell wall extract, we selected three anchorless proteins; ADI, FBA and TF; for further characterization as putative GAS vaccine candidates.…”
Section: Discussionmentioning
confidence: 99%
“…Several previously characterized GAS vaccine candidates; M protein, SfbI, SOF and FbaA [34] are not conserved between GAS serotypes nor expressed by all serotypes of GAS. The genes encoding each of the anchorless vaccine antigens of this study were ubiquitous amongst sequenced GAS genomes with  99% conservation amongst serotypes ( Table 2).…”
Section: Anchorless Proteins Are Highly Conserved and Are Localized Omentioning
confidence: 94%
“…For MALDI-TOF mass spectrometry, 1 l of the digest was mixed with 2 l of the matrix solution (5 mg ␣-cyano-4-hydroxycinnamic acid in 80% [vol/vol] acetonitrile and 0.1% [wt/vol] trifluoroacetic acid [TFA]), and 1 l of this mixture was deposited onto the MALDI target. The spectrum was obtained in the mass range of 500 to 4,000 Da and was calibrated using a calibration mixture containing angiotensin I, Substance P, adrenocorticotropin (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) [ACTH(1-17)], ACTH , and somatostatin (28).…”
Section: Methodsmentioning
confidence: 99%
“…Despite the availability of sequence information for several GAS genomes and detailed characterization of their virulence factors, a safe and effective commercial GAS vaccine has yet to be developed (3).…”
mentioning
confidence: 99%