Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells

Abstract

Human placental extract (HPE) is widely used in Korea to relieve fatigue. However, its effects on human dermal papilla cells (hDPCs) remain unknown. In the present study, in an effort to develop novel therapies to promote hair growth, we screened HPE. We demonstrate that HPE has hair growth‑promoting activities and induces β‑catenin expression through the inhibition of glycogen synthase kinase‑3β (GSK‑3β) by phosphorylation in hDPCs. Treatment with HPE significantly increased the viability of the hDPCs in a concentration‑dependent manner, as shown by bromodeoxyuridine (BrdU) assay. HPE also significantly increased the alkaline phosphatase (ALP) expression levels. The increased β‑catenin levels and the inhibition of GSK‑3β (Ser9) by phosphorylation suggested that HPE promoted the hair-inductive capacity of hDPCs. We compared the effects of treatment with HPE alone and treatment with HPE in conjunction with minoxidil (MXD). We found that HPE plus MXD effectively inhibited GSK‑3β by phosphorylation (Ser9) in the hDPCs. Moreover, we demonstrated that HPE was effective in inducing root hair elongation in rat vibrissa hair follicles, and that treatment with HPE led to a delay in catagen progression. Overall, our findings suggest that HPE promotes hair growth and may thus provide the basis of a novel therapeutic strategy for the clinical treatment of hair loss.

Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth

“…β-Catenin is markedly activated in DPCs during the anagen phase and is important for regeneration of the hair cycle. The inhibition of glycogen synthase kinase 3 (GSK-3) results in the hypo phosphorylation of β-catenin, as well as its increased abundance and nuclear accumulation; this promotes the transcription of growth-promoting gene products cyclin D1 and p21, which are responsible for the cell cycle [ 15 , 50 , 51 ]. Wnt/β-catenin signaling activation by inhibiting GSK-3β maintains the ability of human DPCs to induce hair growth, and β-catenin activity via p-Akt in the DP regulates morphogenesis and the regeneration of hair.…”

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

“…Human placental extract (HPE) possesses Corticotropin‐Releasing Factor (CRF)‐like action, improvement of liver function and anti‐platelet aggregation. PRP and HPE have demonstrated stimulatory effect on the activity of DPCs in vitro .…”

Conditioned medium from primary cytotrophoblasts, primary placenta-derived mesenchymal stem cells, or placental tissue promoted HUVECs angiogenesis in vitro

“…In our study, hPDMSCs-CM contained abundant levels of angiogenic factors and had a proangiogenic effect to a certain extent, and so it would have not only a reduced risk compared to that of cell infusion but also have a certain therapeutic effect. Furthermore, placental tissue-derived CM, similar to placental extract, has also been used in many disease treatments in recent years, such as in ischemic diseases, in scaffolds for engineered tissue [52], to treat osteoarthritis [53], for regenerating sciatic nerves [54], and in burn injuries, chronic ulcers, skin defects [55,56], climacteric symptoms [57], and hair loss [58]. These uses are mainly associated with the biological properties of the placental tissue, such as proangiogenic effects, wound protection [55,56], anti-in ammatory and antioxidative effects, anti-platelet aggregation activity [59,60], anti-aging, and low immunogenicity.…”