Human platelets differentially concentrate estradiol, estrone and testosterone

Sarabia, Stephen F.; Raya, Joe L.; Hoogeveen, Ron C.; Bray, Paul F.

doi:10.1111/j.1538-7836.2008.02898.x

Cited by 5 publications

(7 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…34 Interestingly, comparison of males and premenopausal females found that the plasma E2 and E1 levels were significantly lower in the males, but the platelet E2 and E1 levels were comparable. 34 This raises the possibility that platelet-derived estrogen may be able to act on ILC2s regardless of the gender. In addition, ILC2s from both the uterus and lungs reportedly expressed ESR1, and in vitro E2 stimulation induced gene expression associated with cellular assembly and organization, cell death and survival, and cell cycle and maintenance; these features were more pronounced in ILC2s from the uterus.…”

Section: Discussionmentioning

confidence: 95%

“…Indeed, human platelets reportedly contain various sex hormones, including estrone (E1), 17β‐estradiol (E2) and testosterone 34 . Interestingly, comparison of males and premenopausal females found that the plasma E2 and E1 levels were significantly lower in the males, but the platelet E2 and E1 levels were comparable 34 . This raises the possibility that platelet‐derived estrogen may be able to act on ILC2s regardless of the gender.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Direct platelet adhesion potentiates group 2 innate lymphoid cell functions

Orimo

Tamari

Takeda

et al. 2021

Allergy

View full text Add to dashboard Cite

Background: Platelets are thought to be involved in the pathophysiology of asthma, presumably through direct adhesion to inflammatory cells, including group 2 innate lymphoid cells (ILC2s). Here, we tried to elucidate the effects of platelet adhesion to ILC2s in vitro and in vivo, as well as the mechanisms involved.Methods: Alternaria-induced ILC2-dependent airway inflammation models using wildtype and c-mpl −/− mice were evaluated. Both purified CD41 + and CD41 − ILC2s were cultured with IL-2 and IL-33 to determine in vitro Type 2 (T2) cytokine production and

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Direct platelet adhesion potentiates group 2 innate lymphoid cell functions

Orimo

Tamari

Takeda

et al. 2021

Allergy

View full text Add to dashboard Cite

show abstract

“…Previous reports profiling intraplatelet mRNA content demonstrated the expression of steroidogenic dehydrogenases involved in the production of androgens [18] . Moreover, prior findings that platelets are capable of producing the androgen dehydroepiandrosterone [13] and that platelets from healthy men possess detectable levels of androgens [19] led us to further assay the intraplatelet T biosynthetic pathway in the context of prostate cancer. The data presented in this report demonstrate ( a ) that the full intact T biosynthetic pathway is present within platelets of healthy men and men with CRPC; ( b ) de novo T synthesis from cholesterol within platelets from men with CRPC exhibiting clinical abiraterone resistance, despite ADT; and ( c ) that platelets from men with prostate cancer contain a pool of T sufficient to induce AR signaling in prostate cancer cells ( Supplementary Figure S3 ).…”

Section: Discussionmentioning

confidence: 99%

Platelet-Synthesized Testosterone in Men with Prostate Cancer Induces Androgen Receptor Signaling

et al. 2015

View full text Add to dashboard Cite

Platelets have been long postulated to play a critical role in the pathogenesis of prostate cancer, although relatively little is known regarding the precise mechanisms involved. Androgen deprivation therapy (ADT) for prostate cancer eventually fails with relapse occurring in the form of castration-resistant prostate cancer (CRPC). CRPC tumors typically overexpress androgen receptor (AR), demonstrating continued dependence upon AR signaling. Platelets have been previously demonstrated to contain androgens, and we sought to explore the contribution of platelet-derived androgens in CRPC. In this study, we examined the role of platelet-derived androgens in vitro using platelets from men with CRPC, men with high-risk prostate cancer, and healthy male donors. A series of in vitro assays was performed to elucidate the impact of platelet-derived androgens on androgen-sensitive prostate tumor cells. By examining platelet-derived androgen effects on AR signaling in prostate tumor cells, we found that platelets, from men with CRPC and on ADT, strongly induce AR target genes and tumor cell proliferation. Moreover, we show a fully intact testosterone (T) biosynthetic pathway within platelets from its precursor cholesterol and demonstrate that platelets of CRPC patients with ADT resistance are able to generate T. Overall, our findings reveal an unknown capacity of platelets to synthesize T at functionally relevant levels in patients with lethal prostate cancer. Importantly, it suggests a novel paracrine mechanism of T production that may act to sustain CRPC state and potentiate therapeutic resistance.

show abstract

“…Probably one of the most challenging assumptions that has emerged in recent decades, regarding the effect of androgens on platelet activity, is that platelets are able to uptake and convert sex steroids to their active compounds, or even to generate them from cholesterol under pathological conditions. In 2008, Sarabia et al noted that platelets from healthy men predominantly deposit testosterone, while platelets from healthy premenopausal women predominantly deposit estradiol [178]. They evidenced a tenfold greater plasma testosterone level, and also a more than threefold greater platelet testosterone level, in males than in premenopausal or postmenopausal women [178].…”

Section: Studies Using In Vitro Platelet Function Assaymentioning

confidence: 99%

“…In 2008, Sarabia et al noted that platelets from healthy men predominantly deposit testosterone, while platelets from healthy premenopausal women predominantly deposit estradiol [178]. They evidenced a tenfold greater plasma testosterone level, and also a more than threefold greater platelet testosterone level, in males than in premenopausal or postmenopausal women [178]. It has been previously proven that human platelets express both androgen and estrogen receptors, as well as the steroidogenic dehydrogenases involved in testosterone production, being able to transform androstenedione into potent androgens [147,[179][180][181][182].…”

Section: Studies Using In Vitro Platelet Function Assaymentioning

confidence: 99%

Effects of Exogenous Androgens on Platelet Activity and Their Thrombogenic Potential in Supraphysiological Administration: A Literature Review

Roşca

Vlădăreanu

Mititelu

et al. 2021

JCM

View full text Add to dashboard Cite

Anabolic androgenic steroids (AAS), simply called “androgens”, represent the most widespread drugs used to enhance performance and appearance in a sporting environment. High-dosage and/or long-term AAS administration has been associated frequently with significant alterations in the cardiovascular system, some of these with severe endpoints. The induction of a prothrombotic state is probably the most life-threatening consequence, suggested by numerous case reports in AAS-abusing athletes, and by a considerable number of human and animal studies assessing the influence of exogenous androgens on hemostasis. Despite over fifty years of research, data regarding the thrombogenic potential of exogenous androgens are still scarce. The main reason is the limited possibility of conducting human prospective studies. However, human observational studies conducted in athletes or patients, in vitro human studies, and animal experiments have pointed out that androgens in supraphysiological doses induce enhanced platelet activity and thrombopoiesis, leading to increased platelet aggregation. If this tendency overlaps previously existing coagulation and/or fibrinolysis dysfunctions, it may lead to a thrombotic diathesis, which could explain the multitude of thromboembolic events reported in the AAS-abusing population. The influence of androgen excess on the platelet activity and fluid–coagulant balance remains a subject of debate, urging for supplementary studies in order to clarify the effects on hemostasis, and to provide new compelling evidence for their claimed thrombogenic potential.

show abstract

Human platelets differentially concentrate estradiol, estrone and testosterone

Cited by 5 publications

References 15 publications

Direct platelet adhesion potentiates group 2 innate lymphoid cell functions

Direct platelet adhesion potentiates group 2 innate lymphoid cell functions

Platelet-Synthesized Testosterone in Men with Prostate Cancer Induces Androgen Receptor Signaling

Effects of Exogenous Androgens on Platelet Activity and Their Thrombogenic Potential in Supraphysiological Administration: A Literature Review

Contact Info

Product

Resources

About