Human salivary gland-specific daily variations in histatin concentrations determined by a novel quantitation technique

Gusman, Heloísa; Leone, Cataldo; Helmerhorst, Eva J.; Nunn, Martha E.; Flora, Bianca; Troxler, Robert F.; Oppenheim, Frank G.

doi:10.1016/s0003-9969(03)00182-1

Cited by 41 publications

(42 citation statements)

References 29 publications

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“…The adsorption of histatin 5 was only concentration dependent at low concentrations (≤1 g ml −1 on hydrophilic silica and ≤3 g ml −1 on hydrophobized substrata), and we therefore chose to only investigate concentrations ≤10 g ml −1 , although histatin 5 is known to be present in saliva at concentrations of up to 45 g ml −1 [19]. The amount of histatin 5 on the surfaces corresponded to a monolayer of completely stretched molecules (Tables 1 and 2).…”

Section: Adsorbed Amountsmentioning

confidence: 99%

“…Due to availability reasons we chose to work with lactoferrin from human milk, lactoperoxidase from bovine milk, lysozyme from hen egg and synthetic histatin 5. The experiments in the present study were carried out to investigate and compare the adsorption behaviour of these four proteins, under identical conditions, within the concentration ranges present in saliva (for lactoferrin and lactoperoxidase ≤10 g ml −1 [6,7], lysozyme ≤200 g ml −1 [6], and histatin 5 ≤45 g ml −1 [19]). Previously, studies have been performed on the adsorption of lactoferrin [20,21], lactoperoxidase [22] and lysozyme [20,[23][24][25][26][27], however these publications were carried out using different techniques to some extent, and additionally they were not performed using the same concentration ranges, buffer solutions (or, to some extent, substrates) as the present investigation.…”

Section: Introductionmentioning

confidence: 99%

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Adsorption behaviour and surfactant elution of cationic salivary proteins at solid/liquid interfaces, studied by in situ ellipsometry

Svendsen

Lindh

Arnebrant

2006

Colloids and Surfaces B: Biointerfaces

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Adsorption of the cationic salivary proteins lactoferrin, lactoperoxidase, lysozyme and histatin 5 to pure (hydrophilic) and methylated (hydrophobized) silica surfaces was investigated by in situ ellipsometry. Effects of concentration (≤10 g ml −1 , for lysozyme ≤200 g ml −1 ) and dependence of surface wettability, as well as adsorption kinetics and elutability of adsorbed films by buffer and sodium dodecyl sulphate (SDS) solutions were investigated. Results showed that the amounts adsorbed decreased in the order lactoferrin ≥ lactoperoxidase > lysozyme ≥ histatin 5. On hydrophilic silica, the adsorption was most likely driven by electrostatic interactions, which resulted in adsorbed amounts of lactoferrin that indicated the formation of a monolayer with both side-on and end-on adsorbed molecules. For lactoperoxidase the adsorbed amounts were somewhat higher than an end-on monolayer, lysozyme adsorption showed amounts corresponding to a side-on monolayer, and histatin 5 displayed adsorbed amounts in the range of a side-on monolayer. On hydrophobized substrata, the adsorption was also mediated by hydrophobic interactions, which resulted in lower adsorbed amounts of lactoferrin and lactoperoxidase; closer to side-on monolayer coverage. For both lysozyme and histatin 5 the adsorbed amounts were the same as on the hydrophilic silica. The investigated proteins exhibited fast adsorption kinetics, and the initial kinetics indicated mass transport controlled behaviour at low concentrations on both types of substrates. Buffer rinsing and SDS elution indicated that the proteins in general were more tightly bound to the hydrophobized surface compared to hydrophilic silica. Overall, the surface activity of the investigated proteins implicates their importance in the salivary film formation.

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Section: Adsorbed Amountsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adsorption behaviour and surfactant elution of cationic salivary proteins at solid/liquid interfaces, studied by in situ ellipsometry

Svendsen

Lindh

Arnebrant

2006

Colloids and Surfaces B: Biointerfaces

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“…It has recently been reported that mean histatin (1, 3 and 5) concentrations are almost 3 times higher in SM-SL saliva than in parotid stimulated saliva. Daily variations of salivary histatin concentrations are also detected using a histatin-zinc precipitation method (47). Lysozyme, lactoferrin, and lactoperoxidase show cytotoxic properties against bacteria and fungi, while histatin 5 additionally shows antiviral activity.…”

Section: Histatinsmentioning

confidence: 99%

Antimicrobial Peptides in Saliva and Salivary Glands: Their Roles in the Oral Defense System

et al. 2006

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“…The present study focused on the adsorption behaviour of these two proteins at 10 µg ml −1 , which is within the concentration ranges of these proteins in saliva [10,13]. The adsorption was monitored using in situ ellipsometry which has been used quite extensively for protein adsorption studies (for a review see [14]).…”

Section: Introductionmentioning

confidence: 99%

Lactoperoxidase and Histatin 5 – their Adsorption Behaviour on Silica and Hydrophobized Silica Surfaces, and Implications on their Role in the Initial Salivary Film Formation

Svendsen¹,

Lindh²,

Arnebrant³

2007

Zeitschrift Für Physikalische Chemie

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Lactoperoxidase / Histatin 5 / Ellipsometry / PellicleThe acquired pellicle is the thin salivary film that covers all oral surfaces, formed by selective adsorption of primarily salivary proteins. Several cationic proteins, such as lactoferrin, lactoperoxidase, lysozyme and histatin 5 have been identified in the pellicle. This study focused on the adsorption of lactoperoxidase and histatin 5, to investigate their possible importance in the initial salivary film formation. The adsorption was investigated by means of in situ ellipsometry, to both pure (hydrophilic) and methylated (hydrophobized) silica substrates, at concentrations relevant in saliva. The adsorption was investigated in terms of surface area per molecule and influence of surface wettability. Mass transport controlled adsorption in relation to the initial adsorption from human whole saliva and glandular saliva was also investigated. Results showed that lactoperoxidase adsorbed in larger amounts on pure silica compared to methylated surfaces. Histatin 5 adsorbed to the same extent on the two types of surfaces, but to a lesser extent compared to lactoperoxidase. The mass transport calculated adsorption rates of lactoperoxidase and histatin 5 showed that histatin 5 might potentially have a significant role in the initial adsorption from saliva, whereas lactoperoxidase may also adsorb but is not a dominating component.

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Human salivary gland-specific daily variations in histatin concentrations determined by a novel quantitation technique

Cited by 41 publications

References 29 publications

Adsorption behaviour and surfactant elution of cationic salivary proteins at solid/liquid interfaces, studied by in situ ellipsometry

Adsorption behaviour and surfactant elution of cationic salivary proteins at solid/liquid interfaces, studied by in situ ellipsometry

Antimicrobial Peptides in Saliva and Salivary Glands: Their Roles in the Oral Defense System

Lactoperoxidase and Histatin 5 – their Adsorption Behaviour on Silica and Hydrophobized Silica Surfaces, and Implications on their Role in the Initial Salivary Film Formation

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