Human synovium produces substances that inhibit DNA and stimulate proteoglycan and collagen synthesis by cultured human articular chondrocytes and synovial fibroblasts

Panasyuk, Andrei; Colantuoni, Gladys; Khatib, Abdel‐Majid; Lomri, Abderrahim; Mitrović, D

doi:10.1080/03009740310003758

Cited by 9 publications

(5 citation statements)

References 16 publications

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“…These reports indicate that the synovium in the transitional zone between the articular cartilage and the Synovial cells have been shown to have the ability to differentiate into chondrocytes in vitro [6,8,13]. Nishimura et al [10] described that the synovium harvested from the knees of New Zealand white rabbits was isolated and cultured in agarose suspension with TGF-b, and chondrogenesis was found with type II collagen positive.…”

Section: Discussionmentioning

confidence: 99%

The role of the synovium in repairing cartilage defects

Miyamoto

Deie

Yamasaki

et al. 2007

Knee Surg Sports Traumatol Arthr

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The purpose of this study was to examine the role of the synovium in the transitional zone between the articular cartilage and the synovial membrane in cartilage repair and the relationship between the origin of the repaired cartilage and the grafted synovium. We used 8-week-old Sprague Dawley (SD) rats and green fluorescent protein (GFP) transgenic rats. In study 1, a full-thickness cartilage defect was created at the medial condyle of the femur, and the synovium 5 x 5 mm extending up to the cartilage defect was resected in the left knee (cartilage defect without synovium group) but not resected in the right knee (cartilage defect with intact synovium group). In study 2, after the creation of a full-thickness cartilage defect and resection of the synovium, the synovium of the GFP rats was transplanted into the unilateral knee (cartilage defect with transpl.synovium group). At 2, 4, 6, and 8 weeks after surgery, we evaluated the repaired tissue in cartilage defects histologically and immunohistochemically, and the expression of aggrecan and type II collagen in the repaired tissue was also investigated using reverse transcriptase-polymerase chain reactions (RT-PCR). At 6 and 8 weeks after surgery, the defect was filled with cartilage-like tissue in cartilage defect with intact synovium group and cartilage defect with transpl.synovium group, but not in cartilage defect without synovium group. GFP positive cells were observed in the repaired tissue and the expression of aggrecan and type II collagen was found in cartilage defect with transpl.synovium group. We concluded that the synovium in the transitional zone between the articular cartilage and the grafted synovial membrane invades the cartilage defects where the cells could be detected as GFP-positive cells. Those cells may take part in the repair and may induce chondrogenesis.

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Section: Discussionmentioning

confidence: 99%

The role of the synovium in repairing cartilage defects

Miyamoto

Deie

Yamasaki

et al. 2007

Knee Surg Sports Traumatol Arthr

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“…As we stated in the introduction, in a previous study, we observed that synovium located in the transitional zone between the articular cartilage and the synovial membrane invaded cartilage defects and exhibited chondrogenic potential in vivo [14]. Other studies have shown that synovial cells can differentiate into chondrocytes in vitro [1][2][3]10]. One study harvested synovium from the knees of New Zealand white rabbits, which was then isolated and cultured in agarose suspension with TGF-β, before being identified as type II collagen- Figure 1: Proliferation potential of cultured synovial cells in Group A (in which the synovium was harvested from the transitional zone between the articular cartilage and the synovial membrane at the femoral condyle) and Group B (in which the synovium was harvested from an area 5 mm medial to the transitional zone of the femoral condyle).…”

Section: Discussionmentioning

confidence: 70%

“…They described that the periosteal tissue adjacent to the synoviumcartilage junction has the potential to repair cartilage defects. Thus, recently the role of the synovium in the transitional zone between the articular cartilage and the synovial membrane in cartilage repair has been documented [1,[10][11][12][13]. Those reports indicate that the synovium in the transitional zone between the articular cartilage and the synovial membrane may have the potential to repair cartilage defects.…”

Section: Introduction and Our Previous Researchmentioning

confidence: 99%

Synovium in the Transitional Zone between the Articular Cartilage and the Synovial Membrane Contains Stem Cells and has Greater Chondrogenic Differentiation Potential than Synovium in Other Locations

Adachi¹,

Miyamoto²,

Deie³

et al. 2012

Rheumatology (Sunnyvale)

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Purpose: There were two aims of the present study. (i) to determine differences in chondrogenic potential between synovial cells located in the transitional zone between the articular cartilage and the synovial membrane and those cells in other locations and (ii) to determine the location of mesenchymal stem cells in the synovium. Methods: Synovium was obtained from two different locations in 8-week-old male Sprague-Dawley rats. In Group A, the synovium was harvested from the transitional zone between the articular cartilage and the synovial membrane in the femoral condyle. In Group B, the synovium was harvested from an area 5 mm medial to the transitional zone of the femoral condyle. Synovial cells in both groups were isolated and cultured in monolayer culture system and the proliferation of cells in the first passage was compared between Groups A and B. In addition, passage 3 cells were pellet-cultured to induce chondrogenesis for the evaluation of the chondrogenic potential of the synovial cells. The expression of anti-ATP-binding cassette G-subfamily member 2 (ABCG2), a mesenchymal stem cell marker, was assessed using a real-time polymerase chain reaction in both groups. In a related procedure, both knees of 8-week-old male mice were harvested and cut axially. The synovium in the transitional zone between the cartilage and synovial membrane was examined immunohistochemically by staining with an ABCG2 monoclonal antibody. Results: The synovial cells in Group A demonstrated greater proliferation potential than those in Group B. Synovial cells derived from Groups A showed greater chondrogenic differentiation potential than those in B with respect to pellet size or the intensity of staining with toluidine blue. ABCG-2 expression was significantly higher in Group A than in Group B and ABCG2-stained cells were more prevalent in the synovium of the transitional zone than in the synovium located in other locations. Conclusion: The synovium in the transitional zone between the articular cartilage and the synovial membrane contains mesenchymal stem cells and has a greater chondrogenic differentiation potential than synovium located in other sites.

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“…Substances (glycoproteins, polysaccharides, and lipids) inhibit nucleic acid extraction or amplification in septic joints. 26,27 PCR also may lack sensitivity when a low inoculum of bacteria is present in the sample. This may be exacerbated when only a very small specimen volume (ie, Ͻ20 L) is available.…”

Section: Discussionmentioning

confidence: 99%

Synovial Fluid Changes in Induced Infectious Arthritis in Calves

Francoz

Desrochers

Fecteau

et al. 2005

Journal of Veterinary Internal Medicine

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The objective was to develop an experimental model of septic arthritis in calves and to evaluate the effect of treatment on cytologic and bacteriologic variables of synovial fluid. The right tarsus of 7 healthy Holstein bull calves were inoculated with 10(8) colony-forming units of viable Escherichia coli of a pap-positive strain (day 1). On day 2, joint lavage was performed and antibiotic treatment was instituted. Cytologic examinations, bacterial cultures, and pap factor determinations by polymerase chain reaction (PCR) were performed on synovial fluid samples that were collected daily until day 4, then every 4 days until day 24. Results of physical examination, the severity of lameness, and swelling were recorded. Clinical signs of septic arthritis appeared on day 2 and persisted until day 9 for all calves. Bacterial cultures from all calves were positive for E. coli on day 2, and remained positive until day 3 for 1 calf and until day 4 for 5 calves. In addition, PCR results were positive for all calves, with 6 positive through day 3 and 1 positive through day 4, after which a positive result was again obtained on day 24. Synovial fluid neutrophil counts and white blood cell counts were significantly increased on days 2-4; however, synovial total protein concentrations were increased (P < .05) throughout the experiment in comparison to day 1. Results of all bacterial cultures were negative on day 8, although clinicopathologic signs of inflammation persisted until day 20. This model successfully induced acute septic arthritis in calves. Rapid recovery occurred within 1 week when an appropriate treatment was instituted early in the course of the disease.

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Human synovium produces substances that inhibit DNA and stimulate proteoglycan and collagen synthesis by cultured human articular chondrocytes and synovial fibroblasts

Cited by 9 publications

References 16 publications

The role of the synovium in repairing cartilage defects

The role of the synovium in repairing cartilage defects

Synovium in the Transitional Zone between the Articular Cartilage and the Synovial Membrane Contains Stem Cells and has Greater Chondrogenic Differentiation Potential than Synovium in Other Locations

Synovial Fluid Changes in Induced Infectious Arthritis in Calves

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