Humic acid induces apoptosis in human premyelocytic leukemia HL-60 cells

Yang, Hsin‐Ling; Hseu, You‐Cheng; Hseu, Yi-Ting; Lu, Fung‐Jou; Lin, Eugene; Lai, Jin-Shin

doi:10.1016/j.lfs.2004.02.033

Cited by 34 publications

(19 citation statements)

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“…It has been reported that BMP-2 and -6 gene expression can activate breast cancer and prostate cancer (Zhang et al 2007;Yamamoto et al 2002;Alarmo et al 2008). Generally, FA and humic acid has been known as material that has antioxidative and anti-cancer effect (Tachibana et al 2004;Wang et al 1996;Yang et al 2004). In our previous study, SS-FA had anti-oxidative activity as determined by the results of the DPPH radical scavenging assay.…”

Section: Gene Expression Profile Of Ss-fa Treated Cellsmentioning

confidence: 99%

Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid

et al. 2011

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Fulvic acid (FA) is class of compounds of humic substances formed through the degradation of organic substances by chemical and biological processes. FA has been utilized in traditional Chinese medicine and possesses various pharmacological properties. Previously, we reported that FA extracted from solubilized excess sludge (SS-FA) had an inhibitory effect on b-hexosaminidase release in human leukemia basophilic (KU812) cells. In this study, we investigated the effects of SS-FA on the immediate-type allergic reaction and studied its possible mechanisms of action in KU812 cells following activation with phorbol myristate acetate (20 nmol L -1 ) plus calcium ionophore A23187 (1 lmol L -1 ) (PMACI). The inhibitory effect of SS-FA on degranulation in PMACI-stimulated KU812 cells was examined using histamine release assay. SS-FA significantly decreased the histamine release in KU812 cells at concentrations of 0.1-10.0 lg mL -1 . To gain more information regarding the mechanism of the suppression of degranulation following SS-FA treatment, microarray was conducted to determine which genes were differentially expressed in response to SS-FA in PMACI-activated KU812 cells. From a total of 201 genes in the DNA chip, 28 genes were up-regulated and 173 genes were down-regulated in cells pretreated with SS-FA for 15 min and stimulated with PMACI. From the 71 genes that showed more than two fold change in expression, 16 genes were significantly down-regulated that were subjected to hierarchical clustering. SS-FA affected the expression of genes that were involved in the following pathways: signal transduction, cytokine-cytokine receptor interaction, immune response, cell adhesion molecules and IgE receptor b subunit response.

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Section: Gene Expression Profile Of Ss-fa Treated Cellsmentioning

confidence: 99%

Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid

et al. 2011

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“…S-HA1 and S-HA2 were synthesized from reactive protocatechuic and gallic acid monomers by oxidative polymerization. 11 The raw materials of Y-HA in solid form and W-HA in liquid form were imported from Canada to be used as plant growth auxiliaries. All bulk solid samples were air-dried, powdered, and passed through a 0.2-mm sieve.…”

Section: Chemicals and Hs Samplesmentioning

confidence: 99%

“…Many studies on its cytotoxicity induced by HS, especially HA, have been carried out. [10][11][12] The types and effects of various degraded molecules from HS on pathogenic mechanisms, however, have still not been determined.…”

Section: Introductionmentioning

confidence: 99%

“…HS samples from well waters and manufacturing fertilizers were determined by the optimized CZE following CuO degradation and without further derivatization. Two synthetic HAs, which were composed of gallic and protocatechuic acids, respectively, were previously utilized for cytotoxicity studies, [10][11][12] and were also tested for sample comparison. The quantitative results and derivative parameters of initial tests gave preliminary data in reasonable ranges based on analogous reports.…”

Section: Introductionmentioning

confidence: 99%

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Capillary Electrophoretic Determination of Selected Phenolic Compounds in Humic Substances of Well Waters and Fertilizers

Chen

Chang

et al. 2010

ANAL. SCI.

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In this study, nine oxidized phenolics, which were often found in HS and provide useful information on the taxonomic source and diagenetic state of HS, gallic acid and protocatechuic acid, were employed standard solution to set up the CZE system 2010 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. Humic substances (HS) from well waters, fertilizers, and synthetic phenolic polymers were characterized by elemental and UV-VIS spectroscopic analyses. Capillary zone electrophoresis (CZE) with UV absorption detection was used to analyze the lignin-derived phenolic distribution in the degradation residues after alkaline CuO oxidation of HS samples. Eleven phenols with p-acetyl, vanillyl and syringyl substituents were selected to optimize the CZE parameters. For well waters and fertilizers, the content of phenolic fragments was in agreement with the findings of the elemental and spectroscopic measurements.Additionally, parameters derived from the vanillic acid/vanilline, syringyl acid/syringaldehyde, p-hydroxyl/vanillyl and syringyl/vanillyl ratios matched analogous studies on dissolved organic matter from natural waters and on humic acids from terrestrial substances. The amount of phenolic monomer bonded within two synthetic HS polymers was found to be 25.9% protocatechuic acid and 71.3% gallic acid.

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“…Non-specific effects are physical and chemical membrane irritation, induction and modulation of biotransformation enzymes, induction of stress defense proteins (chaperons), or oxidative stress defense. Also programmed cell death reactions may be induced as observed with human cells (Cheng et al 2003, Hseu et al 2002, Yang et al 2004. Furthermore, (artificial) HS have antiviral, anticoagulant, profibrinolytic, and anti-inflammatory properties (Meerbach et al 2003, Helbig et al 1997, Mahr et al 2003.…”

Section: Introductionmentioning

confidence: 99%

Humic substances

Steinberg

Meinelt

Timofeyev

et al. 2007

Environ Sci Pollut Res

113

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(stress proteins), induction and modulation of biotransformation and anti-oxidant enzymes. Furthermore, they produce an oxidative stress with lipidperoxidation as one clear symptom or even stress defense strategy. Stronger chemical stresses by HS may even lead to teratogenic effects as shown with fish embryos; all physiological responses to HS-mediated stress require energy, which were compensated on the expense of yolk as shown with zebra fish embryos. One Finnish field survey supports the view of a strong chemical stress, as the weight yield in fish species decreases with increasing HS content in the lakes. Discussion. HS exert a variety of stress symptoms in aquatic and compost organisms. According to current paradigms of ecotoxicology, these symptoms have to be considered adverse, because their compensation consumes energy which is deducted from the main metabolism. However, the nematode C. elegans looks actively for such stressful environments, and this behavior is only understandable in the light of new paradigms of aging mechanisms, particularly the Green Theory of Aging. In this respect, we discuss the mild HS-mediated stress to aquatic and compost organisms. New empirical findings with HS themselves and HS building blocks appear to be consistent with this emerging paradigm and show that the individual lifespan may be expanded. At present the ecological consequences of these findings remain obscure. However, a multiple-stress resistance may be acquired which improves the individual fitness in a fluctuating environment.Conclusions. It appears that dissolved HS have to be considered abiotic ecological driving forces, somewhat less obvious than temperature, nutrients, or light.Perspectives. The understanding of the ecological control by dissolved humic substances is still fragmentary and needs to be studied in more details.Keywords: Chemical stress defense; dissolved humic substances; feminization; longevity; membrane irritation; multiple stress resistance; natural herbicides; natural organic matter (NOM); teratogenic effects AbstractGoal, Scope and Background. Freshwater bodies which chemistry is dominated by dissolved humic substances (HS) seem to be the major type on Earth, due to huge non-calcareous geological formations in the Northern Hemisphere and in the tropics. Based on the paradigm of the inertness of being organic, direct interactions of dissolved HS with freshwater organisms are mostly neglected. However, dissolved organic carbon, the majority of which being HS, are natural environmental chemicals and should therefore directly interact with organisms. Major results that widened our perspective on humic substance ecology come from experiments with the compost nematode, Caenorhabditis elegans, which behaved contradictorily to textbook knowledge and provoked an in-depth re-consideration of some paradigms. Approach. To overcome old paradigms on HS and their potential interactions with organisms, we reviewed recent international literature, as well as 'grey' literature. We also include results from...

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Humic acid induces apoptosis in human premyelocytic leukemia HL-60 cells

Cited by 34 publications

References 49 publications

Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid

Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid

Capillary Electrophoretic Determination of Selected Phenolic Compounds in Humic Substances of Well Waters and Fertilizers

Humic substances

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