Hybrid multiphoton and optoacoustic microscope

Tserevelakis, George J.; Soliman, Dominik; Omar, Murad; Ntziachristos, Vasilis

doi:10.1364/ol.39.001819

Cited by 53 publications

(46 citation statements)

References 16 publications

(17 reference statements)

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“…One possibility for future systems would be to combine optical and optoacoustic systems so as to benefit from both the high resolution of an optical microscope and the larger imaging depth of an optoacoustic system. It has already been shown that optoacoustic microscopy can be integrated into an confocal or multiphoton microscope and deliver complementary contrast [83][84][85], but the full potential of combining the two techniques has not been reached yet.…”

Section: (A) (B) (C) (D)mentioning

confidence: 99%

Unleashing Optics and Optoacoustics for Developmental Biology

Ripoll

Koberstein-Schwarz

Ntziachristos

2015

Trends in Biotechnology

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Section: (A) (B) (C) (D)mentioning

confidence: 99%

Unleashing Optics and Optoacoustics for Developmental Biology

Ripoll

Koberstein-Schwarz

Ntziachristos

2015

Trends in Biotechnology

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“…The MP microscope used in this study is similar to one previously described [25]. In brief, MPAF and SHG images were obtained using a properly modified inverted microscope (AxioObserver.D1, Zeiss, Jena, Germany) coupled to an Yb-based solid-state femtosecond laser oscillator, emitting near infrared pulsed light at a central wavelength of 1043 nm (YBIX, Time-Bandwidth, Zurich, Switzerland; pulse width: 170 fs, output average power: 2.8 W, repetition rate: 84.4 MHz).…”

Section: Multi-photon Microscopymentioning

confidence: 99%

Optoacoustic detection of tissue glycation

Ghazaryan

Omar

Tserevelakis

et al. 2015

Biomed. Opt. Express

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Abstract:Oxidative-based diseases including diabetes, chronic renal failure, cardiovascular diseases and neurological disorders are accompanied by accumulation of advanced glycation endproducts (AGE). Therefore, AGE-associated changes in tissue optical properties could yield a viable pathological indicator for disease diagnostics and monitoring. We investigated whether skin glycation could be detected based on absorption changes associated with AGE accumulation using spectral optoacoustic measurements and interrogated the optimal spectral band for skin glycation determination. Glycated and non-glycated skin was optoacoustically measured at multiple wavelengths in the visible region. The detected signals were spectrally processed and compared to measurements of skin autofluorescence and to second harmonic generation multiphoton microscopy images. Optoacoustic measurements are shown to be capable of detecting skin glycolysis based on AGE detection. A linear dependence was observed between optoacoustic intensity and the progression of skin glycation. The findings where corroborated by autofluorescence observations. Detection sensitivity is enhanced by observing normalised tissue spectra. This result points to a ratiometric method for skin glycation detection, specifically at 540 nm and 620 nm. We demonstrate that optoacoustic spectroscopy could be employed to detect AGE accumulation, and possibly can be employed as a non-invasive quick method for monitoring tissue glycation. 14416-14429 (2010). 13. J. Sandby-Møller, T. Poulsen, and H. C. Wulf, "Influence of epidermal thickness, pigmentation and redness on skin autofluorescence," Photochem. Photobiol. 77(6), 616-620 (2003). 14. A. A. Ghazaryan, P. S. Hu, S. J. Chen, H. Y. Tan, and C. Y. Dong, "Spatial and temporal analysis of skin glycation by the use of multiphoton microscopy and spectroscopy," J. Dermatol. Sci. 65(3), 189-195 (2012). 15.

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“…While fluorescence provides a scattering contrast, optoacoustic technology provides an absorption contrast. 111,112 Several systems have been developed for optoacoustic imaging. Portable, hand-held imaging probes can reach 1.5 cm in depth at a speed of 10 volumetric frames per second and a spatial resolution of 200 µm.…”

Section: Optoacoustic/photoacoustic Imagingmentioning

confidence: 99%

Pre- and postmortem imaging of transplanted cells

Andrzejewska

Nowakowski

Janowski

et al. 2015

IJN

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Therapeutic interventions based on the transplantation of stem and progenitor cells have garnered increasing interest. This interest is fueled by successful preclinical studies for indications in many diseases, including the cardiovascular, central nervous, and musculoskeletal system. Further progress in this field is contingent upon access to techniques that facilitate an unambiguous identification and characterization of grafted cells. Such methods are invaluable for optimization of cell delivery, improvement of cell survival, and assessment of the functional integration of grafted cells. Following is a focused overview of the currently available cell detection and tracking methodologies that covers the entire spectrum from pre- to postmortem cell identification.

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Hybrid multiphoton and optoacoustic microscope

Cited by 53 publications

References 16 publications

Unleashing Optics and Optoacoustics for Developmental Biology

Unleashing Optics and Optoacoustics for Developmental Biology

Optoacoustic detection of tissue glycation

Pre- and postmortem imaging of transplanted cells

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