Hybrid Small Animal Imaging System Combining Magnetic Resonance Imaging With Fluorescence Tomography Using Single Photon Avalanche Diode Detectors

Stuker, Florian; Baltes, Christof; Dikaiou, Katerina; Vats, Divya; Carrara, Lucio; Charbon, Edoardo; Ripoll, Jorge; Rudin, Markus

doi:10.1109/tmi.2011.2112669

Cited by 69 publications

(46 citation statements)

References 27 publications

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“…As an alternative, visualization with fluorescence is essentially a surface imaging technique, with limited depth penetration (13,16). Therefore, recent efforts have been made to integrate intraoperative MR imaging with optical imaging in one surgical setting, combining the advantages of two imaging modalities: high soft-tissue contrast and spatial resolution with MR imaging and high sensitivity and real-time data acquisition with optical imaging (17). …”

Section: Methodsmentioning

confidence: 99%

Gliomas: Motexafin Gadolinium-enhanced Molecular MR Imaging and Optical Imaging for Potential Intraoperative Delineation of Tumor Margins

Qiu¹,

zhang²,

Shi³

et al. 2016

Radiology

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Section: Methodsmentioning

confidence: 99%

Gliomas: Motexafin Gadolinium-enhanced Molecular MR Imaging and Optical Imaging for Potential Intraoperative Delineation of Tumor Margins

Qiu¹,

zhang²,

Shi³

et al. 2016

Radiology

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“…In contrast, simultaneous image scanning by both modalities will benefit from the inherent registration due to fixed instrument geometry. 30 According to our previous investigation, 31 the spatial resolution achieved by hybrid FMT-CT was at least 1.2 mm. In our future work, we are planning to simultaneously implement FMT and MR imaging on a hybrid FMT-MRI system, which enables high accuracy FMT reconstruction 21 and offers high soft-tissue contrast, high resolution, and high sensitivity.…”

mentioning

confidence: 89%

“…In our future work, we are planning to simultaneously implement FMT and MR imaging on a hybrid FMT-MRI system, which enables high accuracy FMT reconstruction 21 and offers high soft-tissue contrast, high resolution, and high sensitivity. 30 In comparison with other dual-labeled NPs, IO-anti-EGFR-Cy5.5 NPs show excellent biocompatibility, satisfying colloidal stability, and enhanced MR contrast. 24 Furthermore, Cy5.5, as one representative NIRF dye, possesses the minimum absorbance spectra in the NIR region, 32 and enables characterization of deep diseased tissues.…”

mentioning

confidence: 99%

In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles

Zhang¹,

Zhang²,

Liu³

et al. 2013

IJN

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Dual-modality imaging combines the complementary advantages of different modalities, and offers the prospect of improved preclinical research. The combination of fluorescence imaging and magnetic resonance imaging (MRI) provides cross-validated information and direct comparison between these modalities. Here, we report on the application of a novel tumor-targeted, dual-labeled nanoparticle (NP), utilizing iron oxide as the MRI contrast agent and near infrared (NIR) dye Cy5.5 as the fluorescent agent. Results of in vitro experiments verified the specificity of the NP to tumor cells. In vivo tumor targeting and uptake of the NPs in a mouse model were visualized by fluorescence and MR imaging collected at different time points. Quantitative analysis was carried out to evaluate the efficacy of MRI contrast enhancement. Furthermore, tomographic images were also acquired using both imaging modalities and cross-validated information of tumor location and size between these two modalities was revealed. The results demonstrate that the use of dual-labeled NPs can facilitate the dual-modal detection of tumors, information cross-validation, and direct comparison by combing fluorescence molecular tomography (FMT) and MRI.

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“…FMT and X-ray computed tomography, 7,8,29,32 Stuker et al reported combined MRI and FMT system using single photon avalanche diode detectors. 33 Recently, microscopic positron emission tomography (microPET), with a spatial resolution up to 1 mm, has been used to guide FMT imaging. 30,34 More recently, tri-modality 35 and even pentamodality, tomographic imaging systems were also investigated.…”

Section: Introductionmentioning

confidence: 99%

Anatomical image-guided fluorescence molecular tomography reconstruction using kernel method

et al. 2017

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Abstract. Fluorescence molecular tomography (FMT) is an important in vivo imaging modality to visualize physiological and pathological processes in small animals. However, FMT reconstruction is ill-posed and illconditioned due to strong optical scattering in deep tissues, which results in poor spatial resolution. It is well known that FMT image quality can be improved substantially by applying the structural guidance in the FMT reconstruction. In this paper, a new approach to introducing anatomical information into the FMT reconstruction is presented using the kernel method. In contrast to conventional methods that incorporate anatomical information with a Laplacian-type regularization matrix, the proposed method introduces the anatomical guidance into the projection model of FMT. The primary advantage of the proposed method is that it does not require segmentation of targets in the anatomical images. Numerical simulations and phantom experiments have been performed to demonstrate the proposed approach's feasibility. Numerical simulation results indicate that the proposed kernel method can separate two FMT targets with an edge-to-edge distance of 1 mm and is robust to false positive guidance and inhomogeneity in the anatomical image. For the phantom experiments with two FMT targets, the kernel method has reconstructed both targets successfully, which further validates the proposed kernel method. We have compared the proposed kernel method with the soft prior method thoroughly and found that the kernel method without target segmentation is able to achieve similar anatomical guided results as the soft prior method.

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Hybrid Small Animal Imaging System Combining Magnetic Resonance Imaging With Fluorescence Tomography Using Single Photon Avalanche Diode Detectors

Cited by 69 publications

References 27 publications

Gliomas: Motexafin Gadolinium-enhanced Molecular MR Imaging and Optical Imaging for Potential Intraoperative Delineation of Tumor Margins

Gliomas: Motexafin Gadolinium-enhanced Molecular MR Imaging and Optical Imaging for Potential Intraoperative Delineation of Tumor Margins

In vivo tomographic imaging with fluorescence and MRI using tumor-targeted dual-labeled nanoparticles

Anatomical image-guided fluorescence molecular tomography reconstruction using kernel method

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