Hybridization-based karyotyping of mouse chromosomes: hybridization-bands

Abstract

We have developed a method, which we have named hybridization-banding, to identify simultaneously all chromosomes in a mouse metaphase spread. The method uses a combination of hybridization probes labeled with a single fluor to yield a simple, unique, readily identifiable hybridization pattern on each chromosome. The method is superior to Giemsa- or fluorescence-based banding methods for chromosome identification because the hybridization patterns are simpler and easier to identify, and unique patterns can be designed at will for each chromosome. Analysis can be performed with a standard fluorescence microscope, and images can be recorded on film with an ordinary 35-mm camera, making the method useful to many investigators. The method can also be applied to any species for which chromosomes and probes can be prepared.

Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes

“…The problem, that mouse chromosomes are hard to characterize by GTGbanding and SKY was recognized and tackled previously; i.e. at least two groups developed some kind of YAC/BAC-based chromosome bar code for mouse chromosomes (21)(22)(23)(24). However, no further applications of these approaches were published subsequently.…”

Multicolor fluorescence in situ hybridization (FISH) applied to FISH-banding

“…It produces a unique one-color FISH pattern for each chromosome (Liechty et al, 1999). As a second tactic, the same group published a set of 149 mouse linkage cytogenetics (L-C) probes at 10-cM intervals.…”