Hybridization of Sinapis alba L. and Brassica napus L. via Embryo Rescue

Abstract

Embryo reseue teelmiques were used to otitam hybrids between Sinapis alba L. (white mustard) and Brassiea napus F. (oilseed rape) with the goal ot improving the disease toleranee of oilseed rape. Hybrid plants with 31 or 43 ehromosomes were only recovered, when .S. alba was used as the female parent. One hybrid was obtained from the eross S. alba L. ev. 'Kirby' X B. napus F. ev. 'Topas', while 26 hybrids were obtained, when various .S. alba F. eultivars were pollinated with the rapid cycling B. napus line GrGG 5006. All F| hybrid plants were male sterile; however, ttie first generation baekeross to B. napus L., also obtained hy embryo reseue, produeed plants witti 50 ehromosomes and 61-84 "/o potlen viability. Seeond baekeross generation seed was produeed by normal se.xual erossing. Preliminary eytologieal anatyses of potlen mother cells of hybrid plants suggests the possibility ot genetie e.xchange between the two speeies.

Biology and control of Rhizoctonia solani on rapeseed : A Review

“…SuccessfuI transfer of beet cyst nematode résistance from S. alba to B. napus (Van Lelivelt étal. 1989), and interspecific hybridization between B. juncea and S. alba (Mohapatra and Bajaj 1987), and between B. napus and S. alba (Ripley and Arnison 1990) by means of embryo rescue strengthen this possibility.…”

Behaviour of Sinapis alba chromosomes in a Brassica napus background revealed by genomic in-situ hybridization

“…napus, AACC, 2n = 38), therefore the interspecific transfer of these positive traits to B. napus is desirable to expand the genetic variability for these traits in the more important crop species. Successful sexual hybridization between B. napus and S. alba has been reported through embryo rescue or ovary culture (Ripley & Arnison 1990, Mathias 1991, Lelivelt et al 1993, Chevre et al 1994, Brown et al 1997) and by protoplast fusion (Primard et al 1988, Lelivelt et al 1993, Wang et al 2005b. In our previous work, somatic hybridization was performed between B. napus and S. alba via electrofusion, and seven somatic hybrids were produced (Wang et al 2005b) with the aim of enriching sources of disease resistance of B. napus and introduction of the yellow seed trait from S. alba.…”

Intergeneric Somatic Hybridization Between Brassica napus L. and Sinapis alba L.

“…To date, successful sexual hybridization between B. napus and S. alba has been reported through embryo rescue or ovary culture (Ripley and Arnison 1990;Mathias 1991;Lelivelt et al 1993;Chevre et al 1994;Brown et al 1997) and by protoplast fusion (Primard et al 1988;Lelivelt et al 1993). These somatic hybrids aimed to improve B. napus with resistance to black spot and beet cyst nematode.…”