Hydroalcoholic extract from Baccharis dracunculifolia recovers the gastric ulcerated tissue, and p‐coumaric acid is a pivotal bioactive compound to this action

Abstract

Baccharis dracunculifolia is a medicinal plant native to southeastern Brazil and is the main botanical source used by bees (Apis mellifera) in the manufacture of green propolis and display similar gastroprotective action and chemical profile. This article reports the healing gastric ulcer activity of the hydroethanolic extract of B. dracunculifolia (HEBD) in an acetic acid-induced ulcer model. In addition to the extract, the isolated compounds ferulic acid, p-coumaric acid, caffeic acid, baccharin, and aromadendrin-4'-O-methyl ether were also assayed. HEBD at a dose of 300 mg/kg reduced the ulcerated area by 49.4% after treatment for 7 days, twice a day. Histological analyses revealed that the margins and base of the ulcer obtained significant regeneration, and periodic acid Schiff base staining showed a 78.2% increase in the mucin levels. The action on the enzymatic antioxidant system demonstrated an increased activity of superoxide dismutase and glutathione-S-transferase, in addition to raising glutathione reduced levels and myeloperoxidase activity. HEBD did not show cytotoxicity in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylterazole bromine test. In vitro, HEBD inhibited the H + /K + -ATPase enzyme and showed antioxidant activity in the 2,2 diphenyl-1-picryllydrazyl test. Regarding the isolated compounds, oral administration of p-coumaric acid (15 mg/kg) reduced the ulcerated area by 66.2%. The results suggest that HEBD recovers the gastric ulcerated tissue, raising mucus and antioxidant enzyme levels, and reducing the H + /K + -ATPase activity. In addition, the findings confirm that p-coumaric acid is a pivotal bioactive compound on the gastric healing effects elicited by HEBD. © 2019 BioFactors, 45(3):479-489, 2019

Baccharis dracunculifolia DC Hydroalcoholic Extract Improves Intestinal and Hippocampal Inflammation and Decreases Behavioral Changes of Colitis Mice

“…Automatic gain control was set at 3E6 loads and 250 ms maximum injection time, 70,000 resolutions, and a scan time of 2.5 scans/s. The content of coumaric acid in HEBD was previously verified and was 5% in the dry extract, as Costa et al [ 14 ] described.…”

“…Luceri et al [ 37 ] described that the p-coumaric acid at 50 mg/kg attenuated the intestinal inflammation induced by DSS due to its ability to suppress COX-2 expression and activity. Moreover, our research group showed the gastric healing effects of p-coumaric acid at a 15 mg/kg dose in rats [ 14 ]. Therefore, it is possible to infer that the p-coumaric acid may participate in the intestinal anti-inflammatory effects of the extract along with other constituents and not as an isolated mediator.…”

“…In contrast, the HEBD (300 mg/kg) prevented the decrease in mucin levels even in the intake of DSS. This effect occurred in the stomach of rats submitted to acetic acid–induced ulcer and treated with HEBD (300 mg/kg) [ 14 ], attesting that it favors the mucin secretion in the gastrointestinal tract mucosa and can play a protective factor during injurious processes in these tissues.…”

“…Regarding its anti-inflammatory potential, a few studies have described that B. dracunculifolia mitigates the inflammatory process in macrophages [ 11 ] and in rodents [ 12 ]. Furthermore, Cestari et al [ 13 ] evaluated the intestinal anti-inflammatory of a B. dracunculifolia [ 14 , 15 ] ethyl acetate extract in rats exposed to trinitrobenzene sulfonic acid (TNBS), a model of intestinal inflammation that triggers an immune response like Crohn disease in humans [ 16 ].…”

Estudo Comparativo Da Atividade Antimicrobiana in Vitro De Extratos E Óleo Essencial De Baccharis Dracunculifolia D.C, Contra Escherichia Coli E Staphylococcus Aureus / Comparative Study of the Antimicrobial Activity in Vitamin of Extracts and Essential Oil of Baccharis Dracunculifolia D.C, Against Escherichia Coli and Staphylococcus Aureus

“…Sendo seu cultivo considerado viável e com interessante potencial comercial (GONÇALVES, et al, 2005;GOBBO-NETO;LOPES, 2007;DE SOUSA, et al, 2011;SIMIONI, et al, 2013). Diferentes estudos demostraram a atividade biológica desses metabólicos frente a experimentos de citotoxicidade contra células tumorais, ensaios antivirais, diabetes, ações imunomoduladoras, atividade antioxidante, analgesia, atividade anti-inflamatória, ensaios antiúlceras e antimicrobianos (PEREIRA, 2014;FIGUEIREDO-RINHEL, et al, 2017;CAZELLA et al, 2019;COSTA, 2019. Os antibióticos podem ser compostos de origem natural ou sintéticos, compostos estes capazes de inibir o crescimento ou causar a morte dos microrganismos caracterizando-os como bactericidas ou bacteriostáticos. Depois da descoberta da penicilina e sua industrialização, nos anos de 1940 até 1960, foram descobertos diversas substâncias naturais com atividade antimicrobiana (CALIXTO, CAVALHEIRO, 2012).…”