Hydrodynamics, mass transfer and rheological studies of gibberellic acid production in an airlift bioreactor

Abstract

Although a lot of research has been done into modelling microbial processes, the applicability of these concepts to problems specific for bioreactor design and optimization of process conditions is limited. This is partly due to the tendency to separate the two essential factors of bioreactor modelling, i.e. physical transport processes and microbial kinetics. The deficiencies of these models become especially evident in industrial production processes where O 2 supply is likely to become the limiting factor, e.g. production of gibberellic acid and other organic acids. Hydrodynamics, mass transfer and rheology of gibberellic acid production by Gibberella fujikuroi in an airlift bioreactor is presented in this work. Important hydrodynamic parameters such as gas holdup, liquid velocity in the riser and in the downcomer, and mixing time were determined and correlated with superficial gas velocity in the riser. Mass transfer was studied evaluating the volumetric mass transfer coefficient, which was determined as a function of superficial gas velocity in the riser and as a function of fermentation time. Culture medium rheology was studied through fermentation time and allowed to explain the volumetric mass transfer coefficient behaviour. Rheological behaviour was explained in terms of changes in the morphology of the fungus. Finally, rheological studies let us obtain correlations for gas holdup and volumetric mass transfer coefficient estimation using the superficial gas velocity in the riser and the culture medium apparent viscosity.

Inductive effect produced by a mixture of carbon source in the production of gibberellic acid by Gibberella fujikuroi

“…High levels of preferred nitrogenous compounds can drastically reduce the expression of GAbiosynthetic genes and GA amounts produced. Phosphate, although essential for growth, can at certain concentrations suppress secondary metabolism, inhibiting, for example, phosphatases and oxygenases (Spizek and Tichy 1995;Chávez-Parga et al 2007). In G. fujikuroi the GAs biosynthesis is not regulated by phosphate concentration or rate of growth (Giordano et al 1999).…”

CFD-PBM Coupled Simulation of an Airlift Reactor with Non-Newtonian Fluid

“…The coupled CFD-PBM simulations of ALR are relatively rare, especially with the viscous or non-Newtonian fluid. In industrial applications, many processes [16][17][18] are operated in the viscous mediums or with catalysts showing non-Newtonian behavior.…”

Enhanced production of gibberellin A4 (GA4) by a mutant of Gibberella fujikuroi in wheat gluten medium

“…Commercially, gibberellic acid (GA 3 ) is produced by selected strains of G. fujikuroi using aerobic submerged fermentation, although solid substrate fermentation has been investigated for its production [8,19]. Production of GA 3 by G. fujikuroi is strongly influenced by dissolved oxygen [10], the type of nitrogen source, the carbon source and the pH of the fermentation medium [6].…”