Hydrogel of polysaccharide of sugarcane molasses as carrier of bone morphogenetic protein in the reconstruction of critical bone defects in rats

Abstract

PURPOSE:To evaluate the benefit of using carriers such as the biopolymer gel (hidrogel of polysaccharide of sugarcane molasses) associated with the bone morphogenetic proteins (BMP's) in the repair of critical bone defects in calvaria of Wistar rats. METHODS:Forty-two rats were submitted to a surgical calvaria bone defects. These animals were divided into two experimental groups, positive control group and negative control group. The Group I the calvaria defect was filled up with biopolymer gel, biological membrane, BMP and lyophilized graft. The Group II was treated with biopolymer gel, BMP and lyophilized graft. And the group III (positive control group) was treated with BMP, lyophilized graft and biological membrane. In the negative control group (Group IV) a defect was made in the rat calvaria and the animals were sacrificed immediately after the surgery. The animals of experimental groups and positive control group were slaughtered after subsequent periods of 90 and 180 days. In these periods, the histological analysis and image assessment by cone bean tomographic imaging were obtained. RESULTS:There was highest bone tissue formation with statistically significant results in the groups that associated biopolymer gel and membrane (Group I), followed by the group III (BMP, lyophilized graft and biological membrane). The lower bone formation occurred in the group not using the sugarcane biopolymer gel (Group II). The radiolucent areas of the analyzes of 180 days among the groups studied were respectively, 14.98 mm², 26.65 mm² and 35.81 mm². CONCLUSION:The biopolymer gel showed to be an excellent bone morphogenetic protein carrier, probably by facilitating the controlled release of these proteins in the process of bone repair.

Calcium-phosphate ceramics and polysaccharide-based hydrogel scaffolds combined with mesenchymal stem cell differently support bone repair in rats

“…Various mammalian defect models treated with polysaccharide-derived hydrogels exhibited enhanced tissue or bone repair, as reviewed in [1, 38, 42]. Recently, a novel polymer hydrogel of sugarcane molasses appeared to be a good candidate to treat calvarial bone defects in rats, in association with Bone Morphogenetic Proteins (BMPs) [43]. In patients, hyaluronan-based hydrogels associated with BMP-2 greatly enhanced the healing of critical-size cranial defects [44] or alveolar cleft defects [45], and alginate-agarose hydrogels combined with autologous chondrocytes significantly improved clinical outcome in patients suffering from chondral or osteochondral defects over a 2-year follow-up [46].…”

The biopolymer sugarcane as filling material of critical defects in rats

“…In critical bone defects on calvaria of Wistar rats, SCB gel preparations associated with bone morphogenetic protein (BMP) showed to be a suitable scaffold for the BMP, presumably facilitating the controlled release of BMP during bone repair 11 . Although some studies proposed SCB as a promising material for grafting and other medical procedures, none of those researches aimed bone grafting with granulated SCB to this date [12][13][14][15][16][17] .…”

A New Biomaterial for Urinary Catheters

“…[26] [27]. We chose the in vivo model because the Experimental Surgery Nucleus of our University already has know-how in studies like this [15]- [24]. Besides rats are easy to acquire, enable greater samples, great infections resistance, low cost, and genetic homogeneity.…”

“…Previous studies indicate that it is biocompatible, has low toxicity and induces tissue remodeling [13] [14]. In clinical applications this biomaterial proves to be versatile and has been applied in different experimental studies [15]- [24]. In this context, the aim of this study was to evaluate a SCB performance when in urine contact comparing with a stablished biomaterial.…”