The biopolymer sugarcane as filling material of critical defects in rats

Abstract

PURPOSE:To evaluate the effects of particulate (granule-shaped) SCB on bone repair relating it to its biocompatibility and bone neoformation. METHODS:Thirty Wistar rats were submitted to a one 7-mm-diameter defect and divided equally into three experimental groups, with two different postoperative times of evaluation, 90 and 120 days. Each calvaria defect was filled up with clot (control group), particulated autogenous bone or granulated SCB. Five animals of each group were assessed at 90 and 120 days after surgery. In these two periods, histological and histometric analysis were obtained. RESULTS:The clot group showed a bone resorption trend while the autogenous bone group a bone repair trend. However in the SCB group, the critical defect filled up only with fibrous connective tissue and presented none bone neoformation. CONCLUSION:The sugarcane biopolymer when used in critical size defects was a biocompatible material and proved to be a good material to fill bone cavities, keeping them as uniform areas filled with soft tissue and avoiding the tissue shrinkage.

Effects of bacterial cellulose gel on the anorectal resting pressures in rats submitted to anal sphincter injury

“…The BCG implants were remodeled and remained at the point of implantation in the anorectal region demonstrating biocompatibility with potential to be an efficient volume agent for the treatment of fecal incontinence. These results are similar to those found in other studies that studied cytotoxicity, biocompatibility and remodeling activity of bacterial cellulose [18, 28, 29].…”

Use of bacterial cellulose film for repair of bile duct injury in pigs

“…BCF becomes incorporated into the damaged biliary tissue with an accompanying chronic inflammatory reaction of variable degree. 14,16,17,29,30 Complete absorption of BCF has also been observed 12 weeks after implantation in the tympanic bulla of Wistar rats, and when used to replace, dura mater is gradually absorbed over a period of three months, perhaps owing to use of a thinner film. 31 Similar findings have been reported for extrahepatic ductal grafts composed of a bioabsorbable tube of polylactic acid polymer and polycaprolactone which are completely absorbed by the fourth month.…”

“…Although relative ease of bio-integration and re-modelling has been documented, there are no reports in the literature on use of these biomaterials for repair of bile duct injuries. 14,[16][17][18] Against this background, use of bacterial cellulose membrane for repair of injuries to the extrahepatic biliary ducts represents a promising and potentially cost-effective treatment alternative (note use of these materials for urological procedures). Hence low levels of toxicity for bacterial cellulose coupled with its biocompatibility and proven benefit in experimental models have encouraged its use as a graft material to repair more complex and surgically challenging defects of the bile ducts.…”

Avaliação in Vitro Do Processo De Adesão De Macrófagos J774 Com Arcabouços 3d De Biopolímero De Cana-De-Açúcar

“…Desta forma, promover a adesão de células a um biomaterial pode ajudar na integraçaõ do tecido conectivo mole com o implante, repercutindo no desenvolvimento de novas ferramentas (CARVALHO et al, 2010;MAGER et al, 2011;PIRES et al, 2015). Os biopolímeros despontam como biomateriais inovadores, em função da sua biocompatibilidade e o biopolímero de cana-deaçúcar (BCA), um exopolissacarídeo produzido a partir da fermentação do melaço pela bactéria Zoogloea sp., vem sendo utilizado em diversas aplicações nas áreas das Ciências da Saúde e da Biologia (PATERSON-BEEDLE et al, 2000;CASTRO, et al, 2004;AGUIAR et al, 2007;CAVALCANTE, et al, 2007;SILVEIRA et al, 2007;MAYER et al, 2011;MARTINS et al, 2013;SILVEIRA et al, 2014;ABREU et al, 2016). Em função do exposto, objetivamos avaliar in vitro o processo de adesão de macrófagos J774 com arcabouços 3D a base de biopolímero de cana-de-açúcar.…”