Hydrogen peroxide: A potent activator of dioxygenase activity of soybean lipoxygenase

Kulkarni, Arun P.; Mitra, Ashoke; Chaudhuri, Jayanta; Byczkowski, Janusz Z.; Richards, Ira S.

doi:10.1016/0006-291x(90)91961-q

Cited by 41 publications

(21 citation statements)

References 14 publications

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“…Unsaturated fatty acids may become more and more oxidized and be removed from the lipid bilayer. H,O, has been proposed as being the trigger of the activation of lipoxygenases (Kulkarni et al, 1990). Thus, enhanced degradation of fatty acids that have been released by lipases may occur via the lipoxygenase pathway .…”

Section: Discussionmentioning

confidence: 99%

Correlation of Rapid Cell Death with Metabolic Changes in Fungus-Infected, Cultured Parsley Cells

Naton¹,

Hahlbrock²,

Schmelzer³

1996

Plant Physiology

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To study in detail the hypersensitive reaction, one of the major defense responses of plants against microbial infection, we used a model system of reduced complexity with cultured parsley (Petroselinum crispum) cells infected with the phytopathogenic fungus Phytophthora infestam. Experimental conditions were established to maintain maximal viability of the cultured cells during co-cultivation with fungal germlings, and a large proportion of the infected parsley cells responded to fungal infection with rapid cell death, thereby exhibiting major features of the hypersensitive reaction in whole-plant-pathogen interactions. Rapid cell death clearly correlated with termination of further growth and development of the fungal pathogen. Thus, the system fulfilled important prerequisites for investigating cell-death-related metabolic changes in individual infected cells. Using cytochemical methods, we monitored the increase of mitochondrial activity in single infected cells and the intracellular accumulation of reactive oxygen species prior to the occurrence of rapid cell death. We obtained strong correlative evidence for the involvement of these intracellularly accumulating reactive oxygen species in membrane damage and in the resulting abrupt collapse of the cell.

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Section: Discussionmentioning

confidence: 99%

Correlation of Rapid Cell Death with Metabolic Changes in Fungus-Infected, Cultured Parsley Cells

Naton¹,

Hahlbrock²,

Schmelzer³

1996

Plant Physiology

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show abstract

“…As a second model target we examined the inactivation and nitration of soybean lipoxygenase-1 (LOX), which is known to be inactivated by oxidation induced by various hydroperoxides (20,21). Unlike GAPDH, this enzyme does not contain an essential thiol group.…”

Section: Epicatechin Selectively Protects Against Nitrationmentioning

confidence: 99%

Epicatechin Selectively Prevents Nitration but Not Oxidation Reactions of Peroxynitrite

Schröeder

Klotz

Buchczyk

et al. 2001

Biochemical and Biophysical Research Communications

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“…Its activity is modulated by hy drogen peroxide and nucleotide triphosphates. The effect of H 2 0 2 has been already shown for animal [9] and soybean [37] LOX, and may be related to the ability of this peroxide to convert Fe2+, at the active site of the resting enzyme, to Fe H [8].…”

Section: Discussionmentioning

confidence: 73%

Lipoxygenase activity associated to isolated soybean plasma membranes

Macrı̀

Braidot

Petrussa

et al. 1994

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

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Hydrogen peroxide: A potent activator of dioxygenase activity of soybean lipoxygenase

Cited by 41 publications

References 14 publications

Correlation of Rapid Cell Death with Metabolic Changes in Fungus-Infected, Cultured Parsley Cells

Correlation of Rapid Cell Death with Metabolic Changes in Fungus-Infected, Cultured Parsley Cells

Epicatechin Selectively Prevents Nitration but Not Oxidation Reactions of Peroxynitrite

Lipoxygenase activity associated to isolated soybean plasma membranes

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