Hydrogen peroxide biosensor based on hemoglobin-modified gold nanoparticles–screen printed carbon electrode

Elewi, Ali Saad; Shammaree, Shatha Abdul Wadood AL-; Sammarraie, Abdul Kareem M.A. Al

doi:10.1016/j.sbsr.2020.100340

Cited by 34 publications

(26 citation statements)

References 69 publications

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“…Considering that H 2 O 2 is a product of some oxidases’ catalytic activity [ 26 ], biosensing of H 2 O 2 , based on its electrochemical oxidation/reduction, is frequently favoured by the presence of some specific biological molecules with high peroxidase activity [ 45 ]. Accordingly, H 2 O 2 biosensing is commonly performed using a protein/enzyme-based modified electrode, depending on the biomolecule supported.…”

Section: Hydrogen Peroxide Electrochemical Detectionmentioning

confidence: 99%

“…Accordingly, H 2 O 2 biosensing is commonly performed using a protein/enzyme-based modified electrode, depending on the biomolecule supported. Monitoring H 2 O 2 by its redox reaction becomes faster, more sensitive, and selective when performed in certain heme-proteins such as haemoglobin or other natural enzymes such as the horseradish peroxidase and heme-containing enzymes, such as cytochrome-c peroxidase [ 45 ].…”

Section: Hydrogen Peroxide Electrochemical Detectionmentioning

confidence: 99%

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Low Platinum-Content Electrocatalysts for Highly Sensitive Detection of Endogenously Released H2O2

et al. 2022

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The commercial viability of electrochemical sensors requires high catalytic efficiency electrode materials. A sluggish reaction of the sensor’s primary target species will require a high overpotential and, consequently, an excessive load of catalyst material to be used. Therefore, it is essential to understand nanocatalysts’ fundamental structures and typical catalytic properties to choose the most efficient material according to the biosensor target species. Catalytic activities of Pt-based catalysts have been significantly improved over the decades. Thus, electrodes using platinum nanocatalysts have demonstrated high power densities, with Pt loading considerably reduced on the electrodes. The high surface-to-volume ratio, higher electron transfer rate, and the simple functionalisation process are the main reasons that transition metal NPs have gained much attention in constructing high-sensitivity sensors. This study has designed to describe and highlight the performances of the different Pt-based bimetallic nanoparticles and alloys as an enzyme-free catalytic material for the sensitive electrochemical detection of H2O2. The current analysis may provide a promising platform for the prospective construction of Pt-based electrodes and their affinity matrix.

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Section: Hydrogen Peroxide Electrochemical Detectionmentioning

confidence: 99%

Section: Hydrogen Peroxide Electrochemical Detectionmentioning

confidence: 99%

Low Platinum-Content Electrocatalysts for Highly Sensitive Detection of Endogenously Released H2O2

et al. 2022

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“…14 Ali-saad Elewi et al also developed a biosensor based on hemoglobin immobilized on a screen-printed carbon electrode (SPCE). 15 Despite these advancements, there is still a pressing need to create simple, cost-effective, non-invasive, and quick H 2 O 2 quantification methods. 16…”

Section: Introductionmentioning

confidence: 99%

A smartphone-interfaced, low-cost colorimetry biosensor for selective detection of bronchiectasisviaan artificial neural network

et al. 2022

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“…Direct electrochemistry has been provided for hemoglobin (Hb) immobilized on a nanometer-sized zirconium dioxide (ZrO 2 )-modified pyrolytic graphite (PG) electrode [ 21 ]. Hb was immobilized on screen-printed carbon electrode pre-electrodeposited with gold nanoparticles (AuNPs) [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

A Surface Plasmon Resonance Biosensor Based on Directly Immobilized Hemoglobin and Myoglobin

Dyankov

Borisova

Belina

et al. 2020

Sensors

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Immobilization of proteins on a surface plasmon resonance (SPR) transducer is a delicate procedure since loss of protein bioactivity can occur upon contact with the untreated metal surface. Solution to the problem is the use of an immobilization matrix having a complex structure. However, this is at the expense of biosensor selectivity and sensitivity. It has been shown that the matrix-assisted pulsed laser evaporation (MAPLE) method has been successfully applied for direct immobilization (without a built-in matrix) of proteins, preserving their bioactivity. So far, MAPLE deposition has not been performed on a gold surface as required for SPR biosensors. In this paper we study the impact of direct immobilization of heme proteins (hemoglobin (Hb) and myoglobin (Mb)) on their bioactivity. For the purpose, Hb and Mb were directly immobilized by MAPLE technique on a SPR transducer. The bioactivity of the ligands immobilized in the above-mentioned way was assessed by SPR registration of the molecular reactions of various Hb/Mb functional groups. By SPR we studied the reaction between the beta chain of the Hb molecule and glucose, which shows the structural integrity of the immobilized Hb. A supplementary study of films deposited by FTIR and AFM was provided. The experimental facts showed that direct immobilization of an intact molecule was achieved.

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Hydrogen peroxide biosensor based on hemoglobin-modified gold nanoparticles–screen printed carbon electrode

Cited by 34 publications

References 69 publications

Low Platinum-Content Electrocatalysts for Highly Sensitive Detection of Endogenously Released H2O2

Low Platinum-Content Electrocatalysts for Highly Sensitive Detection of Endogenously Released H2O2

A smartphone-interfaced, low-cost colorimetry biosensor for selective detection of bronchiectasisviaan artificial neural network

A Surface Plasmon Resonance Biosensor Based on Directly Immobilized Hemoglobin and Myoglobin

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