Hydrolysis of cotton fibers by cellulase enzyme

Abstract

SynopsisCotton cellulose has been subjected to continuous and repeated enzymatic hydrolysis for different periods. It has been observed that the length of hydrocellulose particles obtained on repeated enzyme action is reduced to about 300500 8, as compared to 900-3OOO 8, obtained on prolonged enzyme treatment. Corresponding changes in moisture regain, crystallinity of the hydrolysates, and weight loss brought out by the hydrolysis are also discussed.

Changes in the molecular-size distribution of insoluble celluloses by the action of recombinant Cellulomonas fimi cellulases

“…These results suggest that CenA preferentially cleaved completely through the cellulose microfibrils at the amorphous sites, leaving behind predominantly crystalline cellulose. (Although we did not determine crystallinity here, increases in crystallinity of cellulose on enzymic attack have been reported [33][34][35].) Initial peak broadening of both cotton and bacterial cellulose during enzymic attack suggests that CenA cleaved the most accessible amorphous regions of the cellulose first, producing a range of low-molecular-mass fragments.…”

Electron diffraction study of enzyme‐hydrolyzed cotton samples

“…Further, the residue obtained after repeated enzymatic hydrolysis followed by mechanical disintegiation consists of almost crystalline particles of cellulose having length about 350 A. 8 In the present note electron diffraction study on both continuously and repeatedly enzyme-hydrolyzed cotton sample has been reported.…”

Biopolishing of cotton fabrics with total cellulases of Trichoderma reesei and optimization using Taguchi methods

“…Besides the nature of the substrates, efficiency of hydrolysis is also influenced by process conditions and mechanical agitations used in the reaction systems 4–14. Various levels of agitations are used, in practice, using pad‐batch, winch machines, jet systems, and the level of agitations used in the process has profound effects on weight loss values of the fabrics4–10, 15–19 depending upon the structure of yarns, fabrics 18, 20–25…”

“…[1][2][3] Besides the nature of the substrates, efficiency of hydrolysis is also influenced by process conditions and mechanical agitations used in the reaction systems. [4][5][6][7][8][9][10][11][12][13][14] Various levels of agitations are used, in practice, using padbatch, winch machines, jet systems, and the level of agitations used in the process has profound effects on weight loss values of the fabrics [4][5][6][7][8][9][10][15][16][17][18][19] depending upon the structure of yarns, fabrics. 18,[20][21][22][23][24][25] Taguchi's robust design method replicates the experiments with an outer array that deliberately includes the sources of variation (noise factors) that a product or process would come across in service, while such uncontrollable variables are kept under observation during experimentation in many other experimental designs.…”