Hydrophilic interaction chromatography (HILIC) in the analysis of antibiotics

Kahsay, Getu; Song, Heping; Schepdael, Ann Van; Cabooter, Deirdre; Adams, Erwin

doi:10.1016/j.jpba.2013.04.015

Cited by 90 publications

(30 citation statements)

References 113 publications

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“…The concept of HILIC is as follows: a polar stationary phase retains polar analytes that are eluted by a mixture of organic solvent (usually ACN) and water [25]. The main advantages of HILIC comprise: (a) satisfactory retention of polar compounds, (b) increased MS sensitivity due to mobile phase's high organic content which tends to improve desolvation and increase ionization efficiency, (c) better suited for larger volume injection of extracts with high organic content and (d) higher flow rates possible because of the high organic content (lower viscosity) of typical mobile phases [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

“…These groups make them difficult to separate using conventional RPLC without ion pairing. The use of HILIC MS provides the advantage of direct determination (no derivatization or ion-pairing reagents) and higher sensitivity comparing to ion pairing RPLC [25,43]. However, the simultaneous determination of AGs and other drugs in a single method is very difficult due to their rather different physicochemical properties.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of 76 veterinary pharmaceuticals from 13 classes including aminoglycosides in bovine muscle by hydrophilic interaction liquid chromatography–tandem mass spectrometry

Dasenaki

Michali

Thomaidis

2016

Journal of Chromatography A

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of 76 veterinary pharmaceuticals from 13 classes including aminoglycosides in bovine muscle by hydrophilic interaction liquid chromatography–tandem mass spectrometry

Dasenaki

Michali

Thomaidis

2016

Journal of Chromatography A

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“…This can be attributed to the ionization suppressing effect of the IPR which is used in IPLC. In applying HILIC, ZIC columns were generally preferred over anionic and cationic HILIC columns [125].…”

Section: Comparison Between Iplc-ms and Hilic-msmentioning

confidence: 99%

Challenges in the determination of aminoglycoside antibiotics, a review

Farouk

Azzazy

Niessen

2015

Analytica Chimica Acta

122

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“…Usually, a mixture of water and a polar organic solvent such as acetonitrile is used for the mobile phase in HILIC. The retention mechanism of HILIC involves liquid-liquid partitioning between the mobile phase and absorbed water on the surface of the stationary phase [26]. Thus, by increasing the content of water in the mobile phase, the retention in HILIC will decrease.…”

Section: Hydrophilic Interaction Chromatographymentioning

confidence: 99%

Effects of Alignment of Weak Interaction Sites in Molecular Shape Recognition High-Performance Liquid Chromatography

et al. 2016

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This paper introduces organic phases with aligned carbonyl groups derived from L-aspartic acid, L-glutamic acid, and L-α-aminoadipic acid; their stationary phases are denoted as Sil-Asp-2C n , Sil-Glu-2C n , and Sil-Adi-2C n , respectively. The stationary phases were used in high-performance liquid chromatography to investigate the alignment effect of carbonyl groups, which act as π-interaction sites, on molecular shape selectivity. The selectivities of the synthesized organic phases were evaluated using polyaromatic hydrocarbons (PAHs), including geometric isomers (e.g., cis-and trans-stilbenes). The PAH selectivities of the prepared stationary phases were higher than that of conventional octadecyl silica. Among the stationary phases prepared in this study, Sil-Glu-2C n (n = 18) showed the highest selectivity toward terphenyl isomers with different twist configurations and the lowest selectivity toward planar PAHs with different aspect ratios. The results show that the molecular shape selectivity of the phases was affected by the alignment of interaction sites. As a practical application of the octadecylated amino acid derivative-bonded stationary phases, we evaluated their selectivity for tocopherol isomers and achieved good separation. Furthermore, Sil-Asp-2C n (n = 1) showed hydrophilic interaction chromatography mode retention behavior for the separation of polar molecules like nucleosides.

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Hydrophilic interaction chromatography (HILIC) in the analysis of antibiotics

Cited by 90 publications

References 113 publications

Analysis of 76 veterinary pharmaceuticals from 13 classes including aminoglycosides in bovine muscle by hydrophilic interaction liquid chromatography–tandem mass spectrometry

Analysis of 76 veterinary pharmaceuticals from 13 classes including aminoglycosides in bovine muscle by hydrophilic interaction liquid chromatography–tandem mass spectrometry

Challenges in the determination of aminoglycoside antibiotics, a review

Effects of Alignment of Weak Interaction Sites in Molecular Shape Recognition High-Performance Liquid Chromatography

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