Hyperbranched anion exchangers prepared from thiol-ene modified polymeric substrates for suppressed ion chromatography

Abstract

The covalent modification of polymeric particles has been a challenge due to their chemical inertness. Herein we describe a facile and neat method via thiol-ene reaction to immobilize cysteamine/cysteine onto allylmethacrylate-divinylbenzene (AMA-DVB) and ethylvinylbenzene-divinylbenzene (EVB-DVB). The introduced functionalities were verified by elementary analysis and scanning electron microscope coupled with energy dispersive spectroscopy. This modification enabled the further grafting of hyperbranched condensation polymers (HBCPs), which converted the particles into anion exchangers. The anion exchangers demonstrated good separation with carbonate/bicarbonate eluents and hydroxide eluents for suppressed anion exchange chromatography. In the case of a 3-layered anion exchanger, simultaneous separation of nine model anions was achieved by isocratic elution in 17 min with efficiencies up to 16,100 plates m. High stability and dependable durability was exhibited in long-term tests.

A strong anion exchanger of poly(glycidyl methacrylate‐divinylbenzene) substrate functionalized with cationic quaternary ammonium monomer

“…IonPac AS22‐Fast with a particle size of 6.5 μm, slightly larger than that of GH‐MAPTAC used here, the theoretical plate count for chloride is 34 150/m [19]. Similarly, the theoretical plate count of chloride is 25,600 /m in literature [20] and 34 000 /m in literature [21]. The intra‐day and inter‐day RSDs of the retention time of the model anions were 0.23% and 1.48%, respectively.…”

Unusual Nanofractal Microparticles for Rapid Protein Capture and Release

“…For example, noncovalent coating of ionic latex particles, [ 14,15 ] and covalent bonding of ionic small molecules [ 16,17 ] and polymers. [ 18–21 ] However, the interaction sites and multi‐point contacts between surface‐modified microparticles and proteins are dramatically decreased compared with totally porous microparticles, resulting in the reduction of separation efficiency. Therefore, it is still a challenge to simultaneously achieve fast and efficient protein separation.…”