Hyperoxygenated Solution: Effects on Acute Hypobaric Hypoxia-Induced Oxidative Damage in Rabbits

Abstract

High altitude (HA) exposure disrupts the efficiency of the antioxidant system and can lead to oxidative damage in various organs and tissues. The present study investigated the effect of hyperoxygenated solution (HOS) intravenous infusion therapy on oxidative damage induced by acute hypobaric hypoxia. Experimental rabbits were exposed to a simulated high altitude (HA), equivalent to 8500 m, in an animal decompression chamber for 3 h. HOS infusion attenuated the rise in malondialdehyde (MDA) levels and the decrease of the reduced oxidized glutathione (GSH/GSSG) ratio. HOS also increased the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px); the arterial partial pressure of oxygen (Pao(2)); and arterial blood oxygen saturation (Sao(2)) levels. Animals treated with HOS had higher Pao(2) compared with those subjected to airway oxygen therapy (p < 0.01) during HA exposure. These observations suggest that HOS intravenous infusion exerts protective effects against acute hypobaric hypoxia-induced oxidative damage.

The influence of hyperbaric oxygen therapy on intestinal mucosal barrier function in rats with acute carbon monoxide poisoning

“…In the present study, the contents of both ALT and BUN significantly declined after HBO therapy, indicating that HBO may facilitate the recovery of the organs damaged by CO poisoning. This finding is in line with a previous study that indicated that oxygen therapy via intravenous injection alleviates the tissue damage caused by acute hypobaric hypoxia (Zhao et al 2009). …”

The effects of hyperoxia liquid regulate cardiopulmonary bypass‑induced myocardial damage through the Nrf2‑ARE signaling pathway

“…In the present study, resveratrol was suggested the effects of hyperoxia liquid reduced oxidative stress in CPB induced rat model. Zhao et al (18) suggested that hyperoxia liquid protects against acute hypobaric hypoxia-induced oxidative damage. Karu et al (19) reported that the effects of 60 min of hyperoxia followed by normoxia before coronary artery reduced myocardial injury and inflammatory response profile.…”

Hydroxysafflor yellow A exerts antioxidant effects in a rat model of traumatic brain injury

“…The cortical levels of MDA were estimated using the thiobarbituric acid (TBA) method, as described by Zhao et al , with minor modifications ( 21 ). According to the manufacturer's protocol, the supernatant and TBA were mixed together and incubated at 95°C for 40 min.…”

“…The levels of GSH and GSSG in the cortices were determined to assess the redox status. The protocol, according to the specification of the GSH/GSSG assay kit (Nanjing Jiancheng Bioengineering Institute), was previously reported by Zhao et al ( 21 ). The absorbance was recorded at 405 nm on a spectrophotometer and then used to calculate the GSH/GSSG ratio.…”