Hyperthyroidism in the developing rat testis is associated with oxidative stress and hyperphosphorylated vimentin accumulation

Zamoner, Ariane; Barreto, Kátia Padilha; Filho, Danilo Wilhelm; Sell, Fabíola; Woehl, Viviane Mara; Guma, Fátima Costa Rodrigues; Silva, Fátima Regina Mena Barreto; Pessoa-Pureur, Regina

doi:10.1016/j.mce.2007.01.005

Cited by 56 publications

(46 citation statements)

References 83 publications

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“…Indeed, nicotine exposure during lactation causes changes in the hypothalamic-pituitary-thyroid axis, leading to hypothyroidism in adult rats (de Oliveira et al 2011); also, the decrease in T 3 levels is related to hyperphosphorylation of vimentin and concentration of this protein around the Sertoli cell nuclei (Zamoner et al 2007). So, in the present research, the nicotine effects on vimentin expression possibly occurred due to a primary effect on the hypothalamic-pituitary axis.…”

Section: Discussionmentioning

confidence: 51%

Prenatal and lactation nicotine exposure affects Sertoli cell and gonadotropin levels in rats

Paccola¹,

Miraglia²

2016

Reproduction

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Nicotine is largely consumed in the world as a component of cigarettes. It can cross the placenta and reach the milk of smoking mothers. This drug induces apoptosis, affects sex hormone secretion, and leads to male infertility. To investigate the exposure to nicotine during the whole intrauterine and lactation phases in Sertoli cells, pregnant rats received nicotine (2 mg/kg per day) through osmotic minipumps. Male offsprings (30, 60, and 90 days old) had blood collected for hormonal analysis (FSH and LH) and their testes submitted for histophatological study, analysis of the frequency of the stages of seminiferous epithelium cycle, immunolabeling of apoptotic epithelial cells (TUNEL and Fas/FasL), analysis of the function and structure of Sertoli cells (respectively using transferrin and vimentin immunolabeling), and analysis of Sertoli-germ cell junctional molecule (b-catenin immunolabeling). The exposure to nicotine increased the FSH and LH plasmatic levels in adult rats. Although nicotine had not changed the number of apoptotic cells, neither in Fas nor FasL expression, it provoked an intense sloughing of epithelial cells and also altered the frequency of some stages of the seminiferous epithelium cycle. Transferrin and b-catenin expressions were not changed, but vimentin was significantly reduced in the early stages of the seminiferous cycle of the nicotine-exposed adult rats. Thus, we concluded that nicotine exposure during all gestational and lactation periods affects the structure of Sertoli cells by events causing intense germ cell sloughing observed in the tubular lumen and can compromise the fertility of the offspring.

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Section: Discussionmentioning

confidence: 51%

Prenatal and lactation nicotine exposure affects Sertoli cell and gonadotropin levels in rats

Paccola¹,

Miraglia²

2016

Reproduction

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“…Reduced glutathione (GSH) can contribute for the redox potential in the testis and GSH content can be replenished by GGT activity (Zamoner et al 2007;Aprioku 2013;de Liz Oliveira Cavalli et al 2013).…”

Section: Introductionmentioning

confidence: 99%

1,25(OH)2 vitamin D3 signalling on immature rat Sertoli cells: gamma-glutamyl transpeptidase and glucose metabolism

Gonçalves

Zamoner

Zanatta

et al. 2017

J. Cell Commun. Signal.

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1α,25-Dihydroxyvitamin D 3 (1,25-D 3 ) is critical for the maintenance of normal male reproduction since reduced fertility is observed in vitamin D-deficient rats. Gamma-glutamyl transpeptidase (GGT) is a membranebound enzyme that is localized on Sertoli cells and catalyses the transfer of the gamma-glutamyl residues to an amino acid or peptide acceptor. Sertoli cells are also responsible for providing nutrients, as lactate, to the development of germ cells. The aim of this study was to investigate the effect and the mechanism of action of 1,25-D 3 on GGT on Sertoli cell functions from 30-day-old immature rat testis. Results demonstrated that 1,25-D 3 stimulates GGT activity at Sertoli cells plasma membrane through a PKA-dependent mechanism of action, which was not dependent of active de novo protein synthesis. The hormone increases glucose uptake, as well as lactate production and release by Sertoli cells without altering the reactive oxygen species (ROS) generation. In addition, 1,25-D 3 did not change reduced glutathione (GSH) amount or oxygen consumption, and diminished Sertoli cell death. These findings demonstrate that 1,25-D 3 stimulatory effect on GGT activity, glucose uptake, LDH activity and lactate production seem to be an important contribution of Sertoli cells for germ cells nutrition and for a full and active ongoing spermatogenesis.

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“…The effects of these concurrent conditions on different systems of the body have been discussed only as retrospective studies on the basis of some naturally occurring clinical case records in humans, but the data were found lacking in the context of research trials into such a syndrome and its effects on reproductive health. Only few authors have highlighted the role of thyroid hormones in the development of testis and spermatogenesis during adulthood (Zamoner et al, 2007;Sahoo et al, 2008). Considering the situation, this study was planned to mimic such a polyglandular complication by inducing DM concomitantly with hyperthyroidism through injections of streptozotocin (STZ) and levothyroxine (LT4).…”

Section: Introductionmentioning

confidence: 99%

Effects of concomitant diabetes mellitus and hyperthyroidism on testicular and epididymal histoarchitecture and steroidogenesis in male animals

Korejo

Wei

Shah

2016

J. Zhejiang Univ. Sci. B

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This study evaluated the effects of comorbid disorders of diabetes and hyperthyroidism in the adult male mice. In total, 32 ICR strain mice were equally distributed into four groups: control (C), diabetic (D), diabetic-plushyperthyroid (DH), and hyperthyroid (H). Mice allocated for diabetes received a single intraperitoneal injection of streptozotocin (STZ) at 200 mg/kg body weight. At the onset of diabetes, one group of mice was concomitantly injected levothyroxine (LT4; 0.3 mg/kg body weight) and the other set of animals received the same treatment independently on a daily basis. The body weight, as well as the testicular and epididymal weights, was reduced markedly in D and DH mice. Higher trends of blood glucose levels were seen in the DH group, in comparison to euthyroid diabetic mice. Thyroid hormones could exert a transient effect on blood glucose homeostasis by altering the serum blood glucose level in diabetic patients. Histomorphometric analysis showed increased luminal sizes of seminiferous tubules, along with decreased epithelial height and atrophic changes in germinal stem cells in the testis of DH and H mice. Caput epididymis of DH mice showed extensive compaction of principal cells, loss of stereocilia, lipid vacuolization, and inflammatory infiltrations; however, damaged tubular integrity, packed clear cells, exfoliated cells, and round spermatids were profoundly noticed in the cauda epididymis. Hyperthyroidism elevated the serum testosterone levels in H and DH mice and produced critical damages to the histoarchitecture of the epididymis. Collectively, this experiment endeavored to mimic the polyglandular autoimmune syndrome, which will be helpful to better understand the reasons for male infertility in diabetic-cum-hyperthyroid patients.

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Hyperthyroidism in the developing rat testis is associated with oxidative stress and hyperphosphorylated vimentin accumulation

Cited by 56 publications

References 83 publications

Prenatal and lactation nicotine exposure affects Sertoli cell and gonadotropin levels in rats

Prenatal and lactation nicotine exposure affects Sertoli cell and gonadotropin levels in rats

1,25(OH)2 vitamin D3 signalling on immature rat Sertoli cells: gamma-glutamyl transpeptidase and glucose metabolism

Effects of concomitant diabetes mellitus and hyperthyroidism on testicular and epididymal histoarchitecture and steroidogenesis in male animals

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