Hypochlorous acid damages erythrocyte membrane proteins and alters lipid bilayer structure and fluidity

Заводник, И. Б.; Лапшина, Е. А.; Zavodnik, L.B.; Bartosz, Grzegorz; Soszyński, Mirosław; Bryszewska, Maria

doi:10.1016/s0891-5849(00)00479-2

Cited by 50 publications

(36 citation statements)

References 27 publications

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“…It is believed that HClO-induced erythrocyte hemolysis can result from the production of phospholipid chlorohydrins in the cell membrane as well as by formation of nonselective pores [26,38,39]. In our experiments, erythrocyte incubation with 1 mM HClO induced 45.76 ± 2.01% cell hemolysis which was inhibited to the same extent and dose-dependently by preincubation of erythrocytes with both the extracts.…”

Section: Discussionsupporting

confidence: 52%

“…The toxic effects of HClO are connected with the formation of chloramines in reaction with the amino group of lipids, proteins, and also chlorohydrins of lipid degeneration [11,26,38,39]. Erythrocyte exposure to HClO leads to cell swelling, structural rearrangement of the membrane, pore formation, and ultimately to hemolysis [11,26,38]. ONOO -is a reaction product of O 2 .-and NO .…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to ONOO -, HClO does not induce hemoglobin oxidation; however, it induces marked hemolysis [26,38,48]. It is believed that HClO-induced erythrocyte hemolysis can result from the production of phospholipid chlorohydrins in the cell membrane as well as by formation of nonselective pores [26,38,39].…”

Section: Discussionmentioning

confidence: 99%

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Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Olchowik

Lotkowski

Mavlyanov

et al. 2012

Cellular and Molecular Biology Letters

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Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5-50 µg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 µM ONOO -or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

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Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Olchowik

Lotkowski

Mavlyanov

et al. 2012

Cellular and Molecular Biology Letters

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“…The highly reactive biological oxidant hypochlorous acid (HOCl) is generated via the myeloperoxidase-catalysed oxidation of the physiological concentrations of chloride (Cl -) by H 2 O 2 (Zavodnik et al 2001;Winterbourn 2002). HOCl plays an important role in both microbial killing and inflammatory tissue injury by neutrophils (Soszyński et al 2002) and has been implicated also in the diseases or pathological states like ischemia/reperfusion, atherosclerosis, skeletal muscle dysfunction, rheumatoid arthritis and in infammatory conditions Soszyński et al 2002), and is believed to participate in the development of atherosclerotic lesions (Zabe et al 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Oxidation of SH groups, tryptophan (Trp) residues, chloramine formation, changes of membrane fluidity, etc. are events included in injury of ion transport ATPases in erythrocytes induced by HOCl, preceding cell lysis (Zavodnik et al 2001). …”

Section: Introductionmentioning

confidence: 99%

Oxidative injury induced by hypochlorous acid to Ca-ATPase from sarcoplasmic reticulum of skeletal muscle and protective effect of trolox

Štrosová

Karlovska²,

Spickett

et al. 2009

gpb

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Abstract. Hypochlorous acid (HOCl) concentration-dependently decreased ATPase activity and SH groups of pure Ca-ATPase from sarcoplasmic reticulum (SERCA) of rabbit skeletal muscle with IC 50 of 150 µmol/l and 6.6 µmol/l, respectively. This indicates that SH goups were not critical for impairment of Ca-ATPase activity. Pure Ca-ATPase activity was analysed individually with respect to both substrates, Ca 2+

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Molecular and Cellular Concepts in Toxicology

Lynes

2009

General, Applied and Systems Toxicology

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Toxins and toxicants that modify cellular or molecular processes can have repercussions throughout the organism. These interactions can be common to all cell types, or may reflect unique targets or unique bioactivations that are to be found in limited subpopulations of cells. The consequences of these modifications will produce a host of different changes. These changes can be characterized by measurements of transcription factor activity, gene expression at the RNA or protein level, by cellular molecular responses that attempt to re‐establish homoeostasis, and by the consequences to cellular organelle structure and function. This chapter provides an overview of the inter‐relationships between toxicant effect and cellular response, and also describes current technologies that can be used to assess the myriad of changes that may characterize toxin and toxicant exposure at the molecular and cellular levels.

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Hypochlorous acid damages erythrocyte membrane proteins and alters lipid bilayer structure and fluidity

Cited by 50 publications

References 27 publications

Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Oxidative injury induced by hypochlorous acid to Ca-ATPase from sarcoplasmic reticulum of skeletal muscle and protective effect of trolox

Molecular and Cellular Concepts in Toxicology

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