Hypoglycemic Activity and Mechanisms of Extracts from Mulberry Leaves (Folium Mori) and Cortex Mori Radicis in Streptozotocin-Induced Diabetic Mice

Chen, Fujun; Nakashima, Noboru; Kimura, Ikuko; Kimura, Masayasu

doi:10.1248/yakushi1947.115.6_476

Cited by 117 publications

(66 citation statements)

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“…Fasting blood glucose concentrations at the initial and final stages confirm uncontrolled hyperglycemia in untreated diabetic rats, whereas white and black mulberry fruits treatment remarkably decreased blood glucose concentrations in diabetic rats. The effect of white and black mulberry fruits in controlling hyperglycemia could be explained as follow: (I) the N-containing pseudo-sugar isolated form mulberry fruits which inhibit the functions of α-glucosidase, α-mannosidase and β-galactosidase, (II) fagomine which strengthens the glucose induced insulin secretion similar to the action of glibenclamide, a sulfonylurea drug which stimulates the secretion of endogenous insulin, and (III) increase in tissue uptake of glucose by both white and black mulberry fruits [53].…”

Section: Changes In Body Weight and Food Consumptionmentioning

confidence: 99%

Hypoglycemic effect of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits in diabetic rat

et al. 2014

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KEYWORDSThe aim of the present study was to investigate the hypoglycemic effect of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits either used individually or in a combination on alloxan diabetic rats. These fruits are reported to be rich in antioxidants, flavonoids and phenolics that can potentially fight against diabetes mellitus. Male albino rats were divided into 5 groups: normal control, alloxan-diabetic control, diabetic rats treated with white mulberry fruit powder at 5% in the diet, diabetic rats treated with black mulberry fruit powder at 5% in the diet and diabetic rats treated with mixture of white and black mulberry fruits powder at 5% in the diet. After 4 weeks of treatment, blood glucose level, liver and kidney enzymes activity, lipid profile, lipid peroxidation and histopathological studies on liver, kidney and pancreas were evaluated. The mixture of white and black mulberry fruits showed the most significant (p < 0.05) improvement in feed efficiency ratio with increasing body weight gain, as well as decrease in blood glucose level and liver-kidney dysfunction when compared with diabetic control rats. Significant decrease in serum cholesterol, triglycerides and low density lipoprotein cholesterol (LDLc) as well as significant increase in high density lipoprotein cholesterol (HDLc) in diabetic rats was observed with all treatments. Moreover, mulberry fruits administration caused significant inhibition in lipid peroxidation and α-amylase activity. In addition, the beneficial effect of all treatments was further confirmed with histopathological examination of liver, kidney and pancreas. This study reveals hypoglycemic and hypolipidemic effects of white and black mulberry fruits either used individually or in combination as a dietary supplement in alloxan diabetic rats. Alloxan Antidiabetic Hypoglycemic Morus alba fruits Morus nigra fruits Lipid peroxidation

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Section: Changes In Body Weight and Food Consumptionmentioning

confidence: 99%

Hypoglycemic effect of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits in diabetic rat

et al. 2014

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“…It has been reported that mulberry leaf extract has a potent antihyperglycemic effect in mice with streptozotocin-induced diabetes. 29) Iizuka et al 30) reported that Folium mori increases insulin sensitivity and curbs insulin resistance. In the present study, treatment with Folium mori was shown to increase the number of BrdU-positive cells in the dentate gyrus in normal rats and those with STZ-induced diabetes.…”

Section: Discussionmentioning

confidence: 99%

“…[26][27][28] In addition, it has been demonstrated that Folium mori is clinically effective in the treatment and prevention of diabetes. 29) However, no study has been made to date on the effect of Folium mori treatment on the relationship between cell proliferation and NPY expression in the dentate gyrus of diabetes mellitus. The effects of Folium mori on cell proliferation and NPY expression in the dentate gyrus of STZ-induced diabetic rats were thus investigated via immunohistochemistry in the present study.…”

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confidence: 99%

Folium mori Increases Cell Proliferation and Neuropeptide Y Expression in Dentate Gyrus of Streptozotocin-Induced Diabetic Rats.

Kim

Jang

Shin

et al. 2003

Biological & Pharmaceutical Bulletin

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Diabetes mellitus is a common metabolic disorder associated with numerous secondary complications in humans. It has been demonstrated that diabetes results in subtle cerebral disorders 1) including alterations in neurotransmission, electrophysiological abnormalities, and structural changes. [2][3][4] Furthermore, increasing evidence shows that diabetes may be associated with deficits in learning and memory.5) Ryan reported that learning and memory capacities are reduced in Type 1 diabetes mellitus patients. 6) Impairments in cognition, particularly in tasks involving verbal memory or complex information processing was also reported in patients with type 2 diabetes mellitus. 7,8) In addition, it has been demonstrated that streptozotocin (STZ)-induced diabetes significantly reduces the number of proliferating cells in the dentate gyrus of rats. 9)New cell birth and neurogenesis have been demonstrated in the dentate gyrus of several adult mammals including humans.10) Neurogenesis in the dentate gyrus of the hippocampus has been associated with learning and memory formation. [11][12][13] Previous studies have shown that several factors such as enriched environments, learning, seizure, N-methyl-D-aspartate (NMDA) receptor antagonists, serotonin, and physical exercise, and ischemia enhance the proliferation of granular cell precursors and/or neurogenesis in the dentate gyrus 11,12,[14][15][16][17][18] while adrenal steroids, opioid peptides, and stress inhibit it. 15,19,20) In recent years, it has been reported that neuropeptide Y (NPY) is also a factor which is associated with enhanced cell proliferation in the dentate gyrus of rats.21) NPY is a 36-amino acid neuropeptide that is expressed in the central and peripheral nervous systems during development and adulthood. It is known to regulate feeding behavior, gastrointestinal activity, and central cardiovascular functions and to influence seizure threshold and alcohol intake. [22][23][24] Recently, it has been demonstrated that NPY promotes the proliferation of basal cells in the olfactory epithelium of rats, 21) and it has been shown that increases in cell proliferation are closely related to increases in NPY expression in the dentate gyrus in STZ-induced diabetic rats. 25)The various parts of the mulberry tree (Morus alba L.) have been applied in the clinical treatment of various diseases in Oriental medicine. Recent evidence shows that the leaves and shoots from the mulberry tree possess several medicinal properties including hypoglycemic, hypotensive, and diuretic effects. [26][27][28] In addition, it has been demonstrated that Folium mori is clinically effective in the treatment and prevention of diabetes.29) However, no study has been made to date on the effect of Folium mori treatment on the relationship between cell proliferation and NPY expression in the dentate gyrus of diabetes mellitus. The effects of Folium mori on cell proliferation and NPY expression in the dentate gyrus of STZ-induced diabetic rats were thus investigated via immunohistochemistry in th...

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“…In Northeastern Brazil, it is popularly known as "amora-miúra", "amora-preta" or "amoreira". The mulberry belongs to the genus Morus of the family Moraceae (ALMEIDA et al, 2011) and has been widely used in folk medicine as hepatoprotective, hypotensive, antipyretic, analgesic, diuretic, expectorant and antidiabetic (NOMURA, 1988;CHEN et al, 1995). Several compounds have been isolated from the roots, trunk bark and leaves of M. nigra, such as the piperidine alkaloid (moranoline), glycoproteins and phenolic compounds (flavonoids, stilbenes, coumarins) (NOMURA; HANO, 1994;KIM et al, 1999;TOSHIO et al, 2005;MAZIMBA et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effect of ovarian tissue storage in Morus nigra extract on the morphology and DNA fragmentation of ovine preantral follicles

Cavalcante

Barberino

Gouveia

et al. 2017

SCA

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This study demonstrated the effect of Morus nigra leaf extract during ovine ovarian tissue transportation on the survival and apoptosis of preantral follicles in vitro. High Performance Liquid Chromatography (HPLC) was used to determine the fingerprint chromatogram of the crude ethanolic extract. Four pairs of ovaries from four sheep were collected. The ovarian cortex was fragmented and one fragment was fixed in 10% buffered formaldehyde and processed for histological and TUNEL analyses (fresh control). The other fragments were placed in Minimal Essential Medium (MEM -control medium) or M. nigra extract (0.025; 0.05 or 0.1 mg/mL) and stored (simulating transport) at 4ºC for 6, 12 or 24 h. Preserved fragments (6 h) were also destined to histological and TUNEL analyses. HPLC analysis confirmed the presence of antioxidant compounds (rutin, isoquercetin e kaempferitrin) in the extract. There was a decrease (P < 0.05) in the percentage of morphologically normal preantral follicles after preservation in all treatments compared to the fresh control. The percentage of normal preantral follicles after preservation in M. nigra at 0.05 mg/mL for 6 h was higher (P < 0.05) than in MEM or 0.025 mg/ mL M. nigra and similar (P > 0.05) to 0.1 mg/mL of the extract. Apoptosis increased (P < 0.05) after conservation for 6 h in all treatments compared to the fresh control. Moreover, TUNEL positive cells decreased (P < 0.05) after preservation in 0.05 or 0.1 mg/mL M. nigra compared to MEM or 0.025 mg/ mL M. nigra. In conclusion, 0.05 mg/mL M. nigra extract can be used as a preservation medium for ovine ovarian tissue at 4°C for up to 6 h. ResumoEste estudo demonstrou o efeito do extrato das folhas de Morus nigra durante o transporte na sobrevivência e apoptose de folículos pré-antrais in vitro. A CLAE (Cromatografia Líquida de Alta Eficiência) foi usada para determinar a impressão digital cromatográfica do extrato etanólico. Foram coletados 4 pares de ovários de 4 ovelhas. O córtex ovariano foi fragmentado e um dos fragmentos foi fixado em formaldeído tamponado a 10% e destinado às análises histológica e de TUNEL (controle fresco). Os demais fragmentos foram colocados em Meio Essencial Mínimo (MEM -meio controle) ou extrato de M. nigra (0,025, 0,05 ou 0,1 mg/mL) e conservados (simulando transporte) à 4 ºC por 6, 12 ou 24 h. Os fragmentos conservados (6 h) também foram destinados às análises histológica e de TUNEL. A análise de CLAE confirmou a presença de compostos antioxidantes (rutina, isoquercetina e canferitrina) no extrato. Houve uma redução (P < 0,05) na percentagem de folículos pré-antrais morfologicamente normais após conservação em todos os tratamentos em comparação com o controle fresco. A percentagem de folículos pré-antrais normais após conservação em M. nigra a 0,05 mg/mL por 6 h foi maior (P < 0,05) do que em MEM ou M. nigra a 0,025 mg/mL e similar (P > 0,05) ao extrato a 0,1 mg/mL. A apoptose aumentou (P < 0,05) após conservação durante 6 h em todos os tratamentos em comparação com o controle fresco. Além diss...

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Hypoglycemic Activity and Mechanisms of Extracts from Mulberry Leaves (Folium Mori) and Cortex Mori Radicis in Streptozotocin-Induced Diabetic Mice

Cited by 117 publications

References 0 publications

Hypoglycemic effect of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits in diabetic rat

Hypoglycemic effect of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits in diabetic rat

Folium mori Increases Cell Proliferation and Neuropeptide Y Expression in Dentate Gyrus of Streptozotocin-Induced Diabetic Rats.

Effect of ovarian tissue storage in Morus nigra extract on the morphology and DNA fragmentation of ovine preantral follicles

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