Hypoglycemic Effect of Acidic Polysaccharide from <i>Schisandra chinensis</i> on T2D Rats Induced by High-Fat Diet Combined with STZ

Abstract

Polysaccharide is a key bioactive component of Schisandra chinensis and has significant pharmacological activities. The aim of this study was to evaluate the anti-diabetic effect of acidic polysaccharide from Schisandra chinensis (SCAP). Type 2 diabetic (T2D) rats were developed by giving a high-fat diet (HFD) combined with low-dose streptozotocin (STZ), and administered orally with SCAP (25, 50 mg/kg) for 8 weeks. Fasting blood glucose (FBG), fasting insulin (FINS), triglycerides (TG), total cholesterol (TC), lowdensity lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondialdehyde (MDA), and superoxide dismutase (SOD) in the rat's serum were measured. Oral glucose tolerance test (OGTT) and pathological changes of pancreas were observed. Furthermore, expressions of c-Jun N-terminal kinase (JNK), B-cell lymphoma 2-associated X protein (BAX), B-cell lymphoma 2 (Bcl-2), and Cleaved Caspase-3 in pancreatic islet were detected. The results showed that SCAP decreased FBG, TG, TC, LDL-C and MDA levels, increased insulin, HDL-C levels and SOD activity, improved the pathological changes in pancreatic islet. Furthermore, SCAP inhibited the up-regulation of phosphorylated JNK, BAX and Cleaved Caspase-3 proteins, and increased Bcl-2 protein expression. These data indicate that SCAP has a therapeutic effect in T2D rats, and the mechanism may be related to its protection against β-cells apoptosis by regulating apoptosis-related proteins expression to alleviate the injury caused by the oxidative stress.

Efficacy and Safety of Omija (Schisandra chinensis) Extract Mixture on the Improvement of Hyperglycemia: A Randomized, Double-Blind, and Placebo-Controlled Clinical Trial

“…The sample size was calculated using the published data from a previous study [ 19 ] in G-power 3.1, which was performed by including type 2 diabetic patients who were administered a fruit extract for 2 months. The changes in the levels of the main evaluation biomarker (FPG) after 12 weeks of intake in the OSM and placebo groups were assumed as −20.2 and 7.5 mg/dL, respectively.…”

“…SCAP treatment has also been reported to prevent drastic loss in body weight in T2D rats. The available data indicate that SCAP imparts a good therapeutic effect in diabetic rats [ 19 ]. Although Omija has been used as a traditional and medicinal plant, there exists only little information on its effect on glycemic control in humans, apart from its high α-amylase and α-glucosidase inhibitory activity, and postprandial plasma glucose-lowering effect both in vitro and in vivo [ 20 ].…”

Antidiabetic and antioxidant potential of Gardenia latifolia in type-2 diabetic rats fed with high-fat diet plus low-dose streptozotocin

“…Similar findings have been previously reported. 36 Study limitations. Short-term GLE therapy for 2 weeks is one of the study's limitations.…”

Review of the therapeutic effects of the traditional Chinese medicine yuye decoction on diabetes mellitus and its complications

“…Hong et al stated that Schisandra chinensis fruit-supplemented Korean rice cookie called dasik (RCD) had lipid-lowering and antidiabetic effects [20]. It was found that an acidic polysaccharide from Schisandra chinensis had a therapeutic effect on T2D rats by regulating apoptosis-related protein expression to alleviate the injury from oxidative stress [21]. A water-soluble polysaccharide (SSPW1) from Schisandra chinensis had antioxidant activities and anti-diabetic effect on T2D rats [22].…”